The Microtubule Severing Protein UNC-45A Counteracts the Microtubule Straightening Effects of Taxol
Asumi Hoshino,
Valentino Clemente,
Mihir Shetty
et al.
Abstract:UNC-45A is the only known ATP-independent microtubule (MT) severing protein. Thus, it severs MTs via a novel mechanism.In vitroand in cells UNC-45A-mediated MT severing is preceded by the appearance of MT bends. While MTs are stiff biological polymers, in cells, they often curve, and the result of this curving can be breaking off. The contribution of MT severing proteins on MT lattice curvature is largely undefined. Here we show that UNC-45A curves MTs. Usingin vitrobiophysical reconstitution and TIRF microsco… Show more
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