The microsporidian polar tube: evidence for a third polar tube protein (PTP3) in Encephalitozoon cuniculi

Peuvel, Isabelle; Peyret, Pierre; Méténier, Guy; Vivarès, Christian P.; Delbac, Frédéric

doi:10.1016/s0166-6851(02)00073-7

Cited by 72 publications

(92 citation statements)

References 34 publications

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“…By immunoscreening of a cDNA library of E. cuniculi, a third polar tube protein, PTP3, has been identified and cloned (Peuvel et al, 2002). This protein is predicted to be synthesized as a 1256-amino acid precursor (136 kDa) with a cleavable signal peptide and is encoded by a single transcription unit (3990 bp) located on the chromosome XI of E. cuniculi (Peuvel et al, 2002).…”

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

“…This protein is predicted to be synthesized as a 1256-amino acid precursor (136 kDa) with a cleavable signal peptide and is encoded by a single transcription unit (3990 bp) located on the chromosome XI of E. cuniculi (Peuvel et al, 2002). Unlike PTP1 and PTP2, PTP3 is solubilised in the presence of SDS alone without the need for a reducing agent such as DTT (Peuvel et al, 2002). Only one cysteine residue is present, which is located in the potential N-terminal signal peptide.…”

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

“…Only one cysteine residue is present, which is located in the potential N-terminal signal peptide. Charged residues (171 acidic and 144 basic residues) are highly dispersed along the protein (Peuvel et al, 2002). Highly basic N-and C-terminal domains can be distinguished in the sequence of the predicted mature protein (Peuvel et al, 2002).…”

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

“…Charged residues (171 acidic and 144 basic residues) are highly dispersed along the protein (Peuvel et al, 2002). Highly basic N-and C-terminal domains can be distinguished in the sequence of the predicted mature protein (Peuvel et al, 2002). Aspartate and glutamate (16%) are the major amino acids of the protein core (Peuvel et al, 2002).…”

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

“…Highly basic N-and C-terminal domains can be distinguished in the sequence of the predicted mature protein (Peuvel et al, 2002). Aspartate and glutamate (16%) are the major amino acids of the protein core (Peuvel et al, 2002). Immunolocalisation data indicated that PTP3 is involved in the sporoblast-to-spore polar tube biogenesis (Peuvel et al, 2002).…”

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

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The microsporidian polar tube: A highly specialised invasion organelle

Weiss

2005

International Journal for Parasitology

125

111

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All of the members of the Microsporidia possess a unique, highly specialised structure, the polar tube. This article reviews the available data on the organisation, structure and function of this invasion organelle. It was over 100 years ago that Thelohan accurately described the microsporidian polar tube and the triggering of its discharge. In the spore, the polar tube is connected at the anterior end, and then coils around the sporoplasm. Upon appropriate environmental stimulation the polar tube rapidly discharges out of the spore pierces a cell membrane and serves as a conduit for sporoplasm passage into the new host cell. The mechanism of germination of spores, however, remains to be definitively determined. In addition, further studies on the characterisation of the early events in the rupture of the anterior attachment complex, eversion of the polar tube as well as the mechanism of host cell attachment and penetration are needed in order to clarify the function and assembly of this structure. The application of immunological and molecular techniques has resulted in the identification of three polar tube proteins referred to as PTP1, PTP2 and PTP3. The interactions of these identified proteins in the formation and function of the polar tube remain to be determined. Data suggest that PTP1 is an O-mannosylated glycoprotein, a post-translational modification that may be important for its function. With the availability of the Encephalitozoon cuniculi genome it is now possible to apply proteomic techniques to the characterisation of the components of the microsporidian spore and invasion organelle.

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Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

Section: Composition Of the Microsporidian Polar Tubementioning

confidence: 99%

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The microsporidian polar tube: A highly specialised invasion organelle

Weiss

2005

International Journal for Parasitology

125

111

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The Microsporidian Polar Tube and Spore Wall

et al. 2014

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Proteomic analysis of the eukaryotic parasiteEncephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages

et al. 2006

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The microsporidian Encephalitozoon cuniculi is a unicellular obligate intracellular parasite considered as an emerging opportunistic human pathogen. The differentiation phase of its life cycle leads to the formation of stress-resistant spores. The E. cuniculi genome (2.9 Mbp) having been sequenced, we undertook a descriptive proteomic study of a spore-rich cell population isolated from culture supernatants. A combination of 2-DE and 2-DE-free techniques was applied to whole-cell protein extracts. Protein identification was performed using an automated MALDI-TOF-MS platform and a nanoLC-MS/MS instrument. A reference 2-DE map of about 350 major spots with multiple isoforms was obtained, and for the first time in microsporidia, a large set of unique proteins (177) including proteins with unknown function in a proportion of 25.6% was identified. The data are mainly discussed with reference to secretion and spore structural features, energy and carbohydrate metabolism, cell cycle control and parasite survival in the environment.

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The microsporidian polar tube: evidence for a third polar tube protein (PTP3) in Encephalitozoon cuniculi

Cited by 72 publications

References 34 publications

The microsporidian polar tube: A highly specialised invasion organelle

The microsporidian polar tube: A highly specialised invasion organelle

The Microsporidian Polar Tube and Spore Wall

Proteomic analysis of the eukaryotic parasiteEncephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages

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