The microRNA-200 family targets multiple non-small cell lung cancer prognostic markers in H1299 cells and BEAS-2B cells

Pacurari, Maricica; Addison, Joseph; Bondalapati, Naveen; Wan, Ying; Luo, Dajie; Qian, Yong; Castranova, Vincent; Ivanov, Alexey V.; Guo, Nancy Lan

doi:10.3892/ijo.2013.1963

Cited by 77 publications

(83 citation statements)

References 60 publications

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“…In support of this theory, a previous study by Valastyan et al had shown that alteration of miR-31 modulated multiple target genes and affected multiple phenotypes, such as motility, invasion, and resistance to anoikis (Valastyan et al, 2009). Pacurari M et al reported the miR-200 family and these potential targets were functionally involved in canonical pathways of immune response, molecular mechanisms of cancer, metastasis signaling, cell-cell communication, proliferation and DNA repair in Ingenuity pathway analysis (Pacurari et al, 2013). Multiple-to-multiple relationships between miRNAs and targets have been reported by transfection experiments involving human gastric cancer cells (Hashimoto et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the MicroRNA Expression Profile of Cervical Squamous Cell Carcinoma Metastases

Ding¹,

Wu²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Objectives: MicroRNAs (miRNAs) are important regulators of many physiological and pathological processes, including tumorigenesis and metastasis. In this study, we sought to determine the underlying molecular mechanisms of metastatic cervical carcinoma by performing miRNA profiling. Methods: Tissue samples were collected from ten cervical squamous cancer patients who underwent hysterectomy and pelvic lymph node (PLN) dissection in our hospital, including four PLN-positive (metastatic) cases and six PLN-negative (non-metastatic) cases. A miRNA microarray platform with 1223 probes was used to determine the miRNA expression profiles of these two tissue types and case groups. MiRNAs having at least 4-fold differential expression between PLN-positive and PLN-negative cervical cancer tissues were bioinformatically analyzed for target gene prediction. MiRNAs with tumor-associated target genes were validated by quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: Thirty-nine miRNAs were differentially expressed (>4-fold) between the PLN-positive and PLN-negative groups, of which, 22 were up-regulated and 17 were down-regulated. Sixty-nine percent of the miRNAs (27/39) had tumor-associated target genes, and the expression levels of six of those (miR-126, miR-96, miR-144, miR-657, miR-490-5p, and miR-323-3p) were confirmed by quantitative (q)RT-PCR. Conclusions: Six MiRNAs with predicted tumor-associated target genes encoding proteins that are known to be involved in cell adhesion, cytoskeletal remodeling, cell proliferation, cell migration, and apoptosis were identified. These findings suggest that a panel of miRNAs may regulate multiple and various steps of the metastasis cascade by targeting metastasis-associated genes. Since these six miRNAs are predicted to target tumor-associated genes, it is likely that they contribute to the metastatic potential of cervical cancer and may aid in prognosis or molecular therapy.

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Section: Discussionmentioning

confidence: 99%

Characterization of the MicroRNA Expression Profile of Cervical Squamous Cell Carcinoma Metastases

Ding¹,

Wu²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…A variety of markers has been studied or is under investigation in NSCLC in order to thoroughly understand disease progression (26,(41)(42)(43). EMT is one of the pathways for NSCLC progression.…”

Section: Discussionmentioning

confidence: 99%

Epithelial–Mesenchymal Transition in Non Small-cell Lung Cancer

2017

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Abstract. Background/Aim: Lung cancer is the first cause of cancer related deaths in both males and females. Epithelialmesenchymal transition (EMT) is a reversible process by which epithelial cells transform to mesenchymal stem cells by losing their cell polarity and cell-to-cell adhesionLung cancer is the first cause of cancer-related deaths in both males and females. It is a fatal disease and most patients with lung cancer will die of their disease (1). Two main types of lung cancer exist: non-small-cell lung cancer (NSCLC) accounting for 85-90% of lung cancer cases, and small-cell lung cancer (SCLC), whose incidence seems to have decreased over the past decades (2). The current therapeutic approach for lung cancer includes surgical tumor removal, platinum-based chemotherapy, targeted therapy, immunotherapy and radiation therapy. Improvements in clinical and molecular understanding of the disease have resulted in novel therapeutic approaches. Multiple genetic and epigenetic abnormalities results in both oncogene activation [epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK)] and inactivation of tumor-suppressor genes [transcription factor (p53) and retinoblastoma protein (RB)] (3). Moreover, angiogenesis seems to play an important role in the process of invasion and metastasis in lung cancer (4). Other molecular and cellular processes of lung cancer include genomic instability, escape from apoptosis, cell immortalization, abnormalities in immune response and epithelial-mesenchymal transition (EMT) (5-9).

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“…This can occur via a perfect complimentary binding of the miRNA to the target mRNA (endonucleolytic cleavage of the mRNA) or by an imperfect complimentary binding to the target mRNA (translation repression). 32 Due to their cell cycle interference, miRNAs are involved either as oncogenes or as oncosuppressors in the pathogenesis of a huge variety of human cancers such as lung cancer, 33,34 prostate cancer, 35 colorectal cancer, 36,37 leukemia, [38][39][40] gliomas 41 and medulloblastoma, 42 diffuse large B-cell lymphoma, 43 hepatocellular carcinoma (HCC), 44 gastric cancer, 45,46 osteosarcoma, 47 renal cell carcinoma, 48 BC, 49 and OC. 50 Particularly, their presence in the blood has been shown to be associated with histology, clinical stage, survival, and oncogenic expression in OC and BC.…”

Section: Introductionmentioning

confidence: 99%

Beyond circulating microRNA biomarkers: Urinary microRNAs in ovarian and breast cancer

et al. 2017

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Breast cancer is the most common malignancy in women worldwide, and ovarian cancer is the most lethal gynecological malignancy. Women carrying a BRCA1/2 mutation have a very high lifetime risk of developing breast and ovarian cancer. The only effective risk-reducing strategy in BRCA-mutated women is a prophylactic surgery with bilateral mastectomy and bilateral salpingo-oophorectomy. However, many women are reluctant to undergo these prophylactic surgeries due to a consequent mutilated body perception, unfulfilled family planning, and precocious menopause. In these patients, an effective screening strategy is available only for breast cancer, but it only consists in close radiological exams with a significant burden for the health system and a significant distress to the patients. No biomarkers have been shown to effectively detect breast and ovarian cancer at an early stage. MicroRNAs (miRNAs) are key regulatory molecules operating in a post-transcriptional regulation of gene expression. Aberrant expression of miRNAs has been documented in several pathological conditions, including solid tumors, suggesting their involvement in tumorigenesis. miRNAs can be detected in blood and urine and could be used as biomarkers in solid tumors. Encouraging results are emerging in gynecological malignancy as well, and suggest a different pattern of expression of miRNAs in biological fluids of breast and ovarian cancer patients as compared to healthy control. Aim of this study is to highlight the role of the urinary miRNAs which are specifically associated with cancer and to investigate their role in early diagnosis and in determining the prognosis in breast and ovarian cancer.

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The microRNA-200 family targets multiple non-small cell lung cancer prognostic markers in H1299 cells and BEAS-2B cells

Cited by 77 publications

References 60 publications

Characterization of the MicroRNA Expression Profile of Cervical Squamous Cell Carcinoma Metastases

Characterization of the MicroRNA Expression Profile of Cervical Squamous Cell Carcinoma Metastases

Epithelial–Mesenchymal Transition in Non Small-cell Lung Cancer

Beyond circulating microRNA biomarkers: Urinary microRNAs in ovarian and breast cancer

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