The microplate reader: an efficient tool for the separate enzymatic analysis of sugars in plant tissues—validation of a micro‐method

Gomez, Laurent; Bancel, Doriane; Rubio, Emilie; Vercambre, Gilles

doi:10.1002/jsfa.2924

Cited by 83 publications

(62 citation statements)

References 12 publications

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“…The concentrations of Fru, Glc, and Suc were determined within the 163 accessions for the 2 years of sampling using the micromethod developed by Gomez et al (2007). This method is precise, linear, and accurate when compared with HPLC methods.…”

Section: Ssc and Sugarsmentioning

confidence: 99%

Genome-Wide Association in Tomato Reveals 44 Candidate Loci for Fruit Metabolic Traits

Sauvage

Segura

Bauchet³

et al. 2014

Plant Physiology

198

217

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Genome-wide association studies have been successful in identifying genes involved in polygenic traits and are valuable for crop improvement. Tomato (Solanum lycopersicum) is a major crop and is highly appreciated worldwide for its health value. We used a core collection of 163 tomato accessions composed of S. lycopersicum, S. lycopersicum var cerasiforme, and Solanum pimpinellifolium to map loci controlling variation in fruit metabolites. Fruits were phenotyped for a broad range of metabolites, including amino acids, sugars, and ascorbate. In parallel, the accessions were genotyped with 5,995 single-nucleotide polymorphism markers spread over the whole genome. Genome-wide association analysis was conducted on a large set of metabolic traits that were stable over 2 years using a multilocus mixed model as a general method for mapping complex traits in structured populations and applied to tomato. We detected a total of 44 loci that were significantly associated with a total of 19 traits, including sucrose, ascorbate, malate, and citrate levels. These results not only provide a list of candidate loci to be functionally validated but also a powerful analytical approach for finding genetic variants that can be directly used for crop improvement and deciphering the genetic architecture of complex traits.

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Section: Ssc and Sugarsmentioning

confidence: 99%

Genome-Wide Association in Tomato Reveals 44 Candidate Loci for Fruit Metabolic Traits

Sauvage

Segura

Bauchet³

et al. 2014

Plant Physiology

198

217

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“…Total N (Dumas method), starch and total soluble sugar content were estimated by enzymatic analysis (Gomez et al 2007) after drying at 60°C for 3 days.…”

Section: Carbon and Nitrogen Reserves In The Pruned Woodmentioning

confidence: 99%

Changing the soil surface management in vineyards: immediate and delayed effects on the growth and yield of grapevine

et al. 2010

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In a changing context, farmers adapt their cropping systems and, in this respect, the response time of the soil-crop system (changes in the soil resources and in the grapevine vegetative or reproductive development) has to be considered to maintain its agronomic performances. This is particularly true for perennial crops such as grapevine. This paper aims to analyse the short- and long-term effects of changes in soil surface management on grapevine performances. Avineyard in which bare soil inter-rows had been compared with a tall fescue intercrop since 2002 had each of these treatments split into two in 2007, with half of the intercrop being ploughed in and half of the bare soil being sown with fescue, resulting in four treatments whose effects were studied in 2008–2009. Grapevine growth, yield formation and grape quality were assessed. After intercrop destruction, grapevine vegetative growth was enhanced by the mineralization of organic matter from the first year, whereas after intercrop introduction, the competition for soil resources affected growth only in the second year. The grape yield depended on the number of bunches, elaborated the year before. One year after the change in soil surface management, the ranking of yield was as follows: permanent intercrop < intercrop destroyed < intercrop introduced < bare soil. The second year, this ranking altered (still in relation to the number of bunches) to: intercrop introduced < permanent intercrop < bare soil=intercrop destroyed. This study shows that the response times of the processes of grapevine vegetative growth and yield formation to a change in the cropping system vary from one to two years, and therefore, have to be considered carefully to manage the cropping system well

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“…The total N concentration was measured on an elemental analyzer (Flash EA 1112, Thermo Finnigan Milan, Italy). Soluble sugars and starch were determined as described by Gomez et al (2007) and then added together to compute the total non-structural carbon (TNC) pool. Tree N and TNC contents (g organ -1 ) were calculated from organ DW, N and TNC concentrations (% DW).…”

Section: Plant Analysismentioning

confidence: 99%

Autumnal nitrogen nutrition affects the C and N storage and architecture of young peach trees

Jordan

Vercambre

Bot

et al. 2010

Trees

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Nitrogen fertilisation is a regular practice in orchards. Its effect on tree development, N and C acquisition and allocation were evaluated simultaneously, while coupling on the same trees in situ measurements of N uptake and shoot development and destructive determinations of organ composition in N and Total Non structural Carbohydrates (TNC). An hydroponic set-up was designed that could grow young peach trees at constant NO 3 concentration while measuring N uptake. Forty-eight trees were raised outdoors under excessive N supply. Between October 2 and December 7, half of them were then N-limited to reduce N uptake by 75%. Organ N concentrations remained stable in the controls but were halved in N-limited trees. Growth (390 vs. 353 g DW tree -1 ) was less affected by the treatment than N uptake (10.6 vs. 2.7 g N tree -1 ). Growth was affected only in terms of axillary bud development, which was restricted to the median and upper crown parts. The number of buds which transformed into elongating axes (44 vs. 84 tree -1 ) was halved, thus reducing leaf area by one-third (10,464 vs. 15,568 cm 2 ). Tree TNC content was not impacted. The difference in C acquisition likely balanced the C costs of N uptake. In N-limited trees, more TNC was stored as starch (73 vs. 56%), and the allocation patterns of TNC and N were altered in favour of the roots. Our results provide deeper insights into the tree integrated response to autumnal N fertilisation, focusing on an alteration of the balance between storage and growth.

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The microplate reader: an efficient tool for the separate enzymatic analysis of sugars in plant tissues—validation of a micro‐method

Cited by 83 publications

References 12 publications

Genome-Wide Association in Tomato Reveals 44 Candidate Loci for Fruit Metabolic Traits

Genome-Wide Association in Tomato Reveals 44 Candidate Loci for Fruit Metabolic Traits

Changing the soil surface management in vineyards: immediate and delayed effects on the growth and yield of grapevine

Autumnal nitrogen nutrition affects the C and N storage and architecture of young peach trees

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