The micronucleus test: temporal pattern of baseline frequency in Mytilus galloprovincialis

Brunetti, Riccardo; Gabriele, M.; Valerio, P.; Fumagalli, O.

doi:10.3354/meps083075

Cited by 36 publications

(11 citation statements)

References 2 publications

(5 reference statements)

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“…Though baseline frequency of MN depends on variety of biotic and abiotic factors (Brunetti et al, 1988), it can be assumed that higher water temperature in the offshore areas of the Baltic and the North Sea affects mitotic activity and MN formation in fish. Micronuclei are formed during cell division, and the mitotic activity of poikilotherm organisms very largely depends on water temperature (Brunetti et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Rybakovas

Baršienė

Lang

2009

Marine Environmental Research

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Please cite this article as: Rybakovas, A., Baršienė, J., Lang, T., Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and North Seas, Marine Environmental Research (2009Research ( ), doi: 10.1016Research ( /j.marenvres. 2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractMicronuclei (MN), nuclear buds (NB) and fragmented-apoptotic cells (FA) were analyzed in mature peripheral blood and immature cephalic kidney erythrocytes of flounder (Platichthys flesus), dab (Limanda limanda) and cod (Gadus morhua) from 12 offshore sites in the Baltic Sea (479 specimens) and 11 sites in the North Sea (291 specimens), which were collected during three research vessel cruises in December 2002 and in September 2004. The highest levels of environmental genotoxicity (frequencies of MN up to 0.5 ‰, NB -up to 0.75 ‰) and cytotoxicity (FA -up to 0.53 ‰) were observed in flatfishes from areas close to oil and gas platforms in the North Sea and in zones related to the extensive shipping and potentially influenced by contamination from large European Rivers (Elbe, Vistula, Oder). In dab from the offshore zones of the North Sea, the levels of nuclear abnormalities were higher as compared to those in dab from the Baltic Sea. Responses in immature kidney erythrocytes were higher than in mature erythrocytes from peripheral blood. MN frequency lower than 0.05 ‰ (the Baltic Sea) and lower than 0.1 ‰ (the North Sea) could be suspected as a reference level in the peripheral blood erythrocytes of flatfish.Keywords: Genotoxicity; Cytotoxicity; Micronuclei; Nuclear abnormalities; Erythrocytes; Baltic Sea; North Sea. ACCEPTED MANUSCRIPT 2 IntroductionSurrounded by European countries, the Baltic and the North Seas are constantly exposed to anthropogenic pressure. With pollution from land-based discharges, coastal industries, municipal outflows, agricultural activities, oil and gas industry (drilling and transportation) and transport activities, thousands of chemicals are entering the marine environment. A large proportion of these compounds are potentially toxic, genotoxic and carcinogenic to aquatic organisms (Jha, 2004(Jha, , 2008. The interaction of genotoxic contaminants with DNA causes various genetic disturbances, which often are irreversible and can be transmitted to the next generations (Depledge and Fossi, 1994;Dixon et al., 1999;Jha, 2004). The analysis of environmental genotoxicity provides early warning signs of adverse long-term effects of contamination. A number of biomarkers have been applied for assessment of environmental genotoxicity and the micronuclei (MN) test,...

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Section: Discussionmentioning

confidence: 99%

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Rybakovas

Baršienė

Lang

2009

Marine Environmental Research

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“…Although pollutants can cause genetic damage in M. edulis (e.g. Brunetti et al 1992), Sirover & Loeb (1976 and Nacci et al (1992) indicate that copper has limited genotoxicity. Pre-exposure to small amounts of copper can increase the resistance of adult mussels to higher concentrations (Davenport & Manley 1978, Roesijadi & Fellingham 1987, probably by stimulating the synthesis of metallothionein (Viarengo et al 1980, Viarengo 1989.…”

Section: Introductionmentioning

confidence: 99%

Variation among populations in the resistance of Mytilus edulis embryos to copper:adaptation to pollution?

Hoare¹,

Beaumont²,

Davenport³

1995

Mar. Ecol. Prog. Ser.

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The effects of copper on embryo development were found to differ between populations of the mussel Mytilus edulis L. (Mollusca: Bivalvia). Concentrations of copper which caused significantly increased rates of abnormality, and thus decreased yields of normal larvae, in populations from an unpolluted site (Menai Straits, Wales, UK) and to a lesser extent in an intermediately polluted site (Oosterschelde, The Netherlands) did not affect development of embryos from a polluted-site (Westerschelde, The Netherlands) population Crosses indicated that tolerance was mostly maternally determined, but there is also evidence of a paternal, i.e, genetic, effect. An appreciable quantity of background, non-treatment, embryo abnormality appeared to be sperm-mediated.

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“…In this context, Brunetti et al (1992) suggested a considerable influence of temperature on MN frequency. Analysing the current temporal pattern of ENA frequency is not discernible a clear influence of temperature on its expression.…”

Section: Ena Frequencymentioning

confidence: 97%

Biotransformation and Genotoxic Biomarkers in Mullet Species (LIZA SP.) From a Contaminated Coastal Lagoon (Ria De Aveiro, Portugal)

Pacheco

Santos

Teles

et al. 2005

Environ Monit Assess

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The aim of the present study was to assess liver ethoxyresorufin-O-deethylase (EROD) activity and erythrocytic nuclear abnormalities (ENA) frequency in three mullet species, as biotransformation and genotoxicity biomarkers, respectively, for monitoring aquatic ecosystems. Specimens of Liza aurata, Liza ramada and Liza saliens were caught bimonthly, between May 2000 and March 2001, at nine stations in the Ria de Aveiro (Portugal), a polluted coastal lagoon. The survey revealed two critical areas (Vagos and Carregal) where it was observed elevated EROD activity and ENA frequency, comparing to the reference sites. The results often reflect a contamination by a mixture of pollutants, from point and non-point sources. Nevertheless, the responses observed at Vagos are probably attributable to high polycyclic aromatic hydrocarbon (PAH) water levels measured at that station. All species were able to detect inter-site differences; however L. aurata seems to be the most appropriate bio-indicator due to its larger geographical distribution and abundance during the whole year. The adopted biomarkers, applied to mullet species, can be successfully employed for biomonitoring anthropogenic contamination in both Atlantic and Mediterranean European coastal waters.

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The micronucleus test: temporal pattern of baseline frequency in Mytilus galloprovincialis

Abstract: ABSTRACT. The base-line frequency of micronucleated cells was measured in g111 tissue of Mytilus galloprov~nc~ahs Lmk. from 2 ~Mediterranean areas (northern Tyrrhenian and northern Adriatic Seas), and turned out to be a function of water temperature and age of the animals.

Cited by 36 publications

References 2 publications

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Variation among populations in the resistance of Mytilus edulis embryos to copper:adaptation to pollution?

Biotransformation and Genotoxic Biomarkers in Mullet Species (LIZA SP.) From a Contaminated Coastal Lagoon (Ria De Aveiro, Portugal)

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