The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides

Hayashi, Makoto; Morita, Takeshi; Kodama, Yukio; Sofuni, Toshio; Ishidate, Motoi

doi:10.1016/0165-7992(90)90153-b

Cited by 444 publications

(203 citation statements)

References 4 publications

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“…The MA used in this experiment was originally described by Hayashi et al (1990) with modifications by Oliveira et al (2009). The slides were warmed to 70°C and covered with 20 μL acridine orange in an aqueous solution (1.0 mg/mL).…”

Section: Mamentioning

confidence: 99%

Antimutagenic and anticarcinogenic effects of wheat bran in vivo

Pesarini¹,

Zaninetti²,

Mauro³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Previous studies in rodents treated with the pro-carcinogen 1,2-dimethylhydrazine suggested that the consumption of wheat bran protected against DNA damage in the colon and rectum. Based on this information, we evaluated wheat bran as a functional food in the prevention and treatment of colon cancer. We used the aberrant crypt focus assay to evaluate the anticarcinogenic potential of wheat bran (Triticum aestivum Dietary wheat bran chemoprevention in vivo variety CD-104), the comet assay to evaluate its antigenotoxicity potential, and the micronucleus assay to evaluate its antimutagenic potential. The wheat bran gave good antimutagenic and anticarcinogenic responses; the DNA damage decreased from 90.30 to 26.37% and from 63.35 to 28.73%, respectively. However, the wheat bran did not significantly reduce genotoxicity. Further tests will be necessary, including tests in human beings, before this functional food can be recommended as an adjunct in the prevention and treatment of colon cancer.

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Section: Mamentioning

confidence: 99%

Antimutagenic and anticarcinogenic effects of wheat bran in vivo

Pesarini¹,

Zaninetti²,

Mauro³

et al. 2013

Genet. Mol. Res.

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“…The MA was performed using the original protocol described by Hayashi et al (1990), with the modifications described by Oliveira et al (2009). The slides were warmed to 70°C and covered with a layer of 20 μL 1.0 mg/mL acridine orange in an aqueous solution.…”

Section: Mamentioning

confidence: 99%

Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo

Mauro¹,

Monreal²,

Silva³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The incidence of colorectal cancer is growing worldwide. The characterization of compounds present in the human diet that can prevent the occurrence of colorectal tumors is vital. The oligosaccharide inulin is such a compound. The aim of this study was to evaluate the antigenotoxic, antimutagenic and anticarcinogenic effects of inulin in vivo. Our study is based on 3 assays that are widely used to evaluate chemoprevention (comet assay, micronucleus assay, and aberrant crypt focus assay) and tests 4 protocols of treatment with inulin (pre-treatment, simultaneous, posttreatment, and pre + continuous). Experiments were carried out in Swiss male mice of reproductive age. In order to induce DNA damage, we used the pro-carcinogenic agent 1,2-dimethylhydrazine. Inulin was administered orally at a concentration of 50 mg/kg body weight following the protocols mentioned above. Inulin was not administered to the control groups. Our data from the micronucleus assay reveal antimutagenic effects of inulin in all protocols. The percentage of inulin-induced damage reduction ranged from 47.25 to 141.75% across protocols. These data suggest that inulin could act through desmutagenic and bio-antimutagenic mechanisms. The anticarcinogenic activity (aberrant crypt focus assay) of inulin was observed in all protocols and the percentages of damage reduction ranged from 55.78 to 87.56% across protocols. Further tests, including human trials, will be necessary before this functional food can be proven to be effective in the prevention and treatment of colon cancer.

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“…The micronucleus test on peripheral blood cells was carried out as described by Hayashi et al (1990), who used slides prestained with acridine orange. Glass slides were heated to about 70 ºC on a hot-plate and a 10 µL drop of an aqueous solution of the dye (1 mg/mL) was placed on each slide and spread evenly over the surface with the end of a second well-cleaned slide.…”

Section: Micronucleus Testmentioning

confidence: 99%

Mutagenicity of two species of the genus Alchornea measured by Salmonella microsome assay and micronucleus test

Santos¹,

Calvo²,

Cólus³

et al. 2010

Rev. bras. farmacogn.

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Some species of the plant genus Alchornea (family Euphorbiaceae) are widely used in popular medicine, mainly in South America and in Africa. Several kinds of biological activity have been seen in the species: antioxidant, antifungal, anti-inflammatory, antibacterial, cytotoxic against tumor cell lines and inhibitory to the replication of HIV-1 and HIV-2. In Brazil, the species Alchornea castaneaefolia Willd. A. Juss. and Alchornea glandulosa Poepp. & Endl. are used by the local population to treat rheumatism, arthritis and muscular pains. In view of the popular use of these plants as medicines and the potential risks from their consumption, we assessed the mutagenic potential of chloroform and methanol extracts of the leaves of these plant species, employing the in vivo micronucleus test and the Ames assay. The data obtained showed that the chloroform extracts were not mutagenic. The methanol extract of A. castaneaefolia was mutagenic to strain TA98 of Salmonella typhimurium and the methanol extract of A. glandulosa to strains TA98 and TA97a. The methanol extracts of both species of Alchornea were mutagenic in vivo at the largest dose employed. The probable mutagenic agents involved were the aglycone quercetin and amentoflavone, present in both species.

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The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides

Cited by 444 publications

References 4 publications

Antimutagenic and anticarcinogenic effects of wheat bran in vivo

Antimutagenic and anticarcinogenic effects of wheat bran in vivo

Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo

Mutagenicity of two species of the genus Alchornea measured by Salmonella microsome assay and micronucleus test

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