The Microneme Proteins EtMIC4 and EtMIC5 of Eimeria tenella Form a Novel, Ultra-high Molecular Mass Protein Complex That Binds Target Host Cells

Periz, Javier; Gill, Andrew C.; Hunt, Lawrence; Brown, Philip J.; Tomley, Fiona M.

doi:10.1074/jbc.m702407200

Cited by 29 publications

(28 citation statements)

References 45 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The EST database and genomic data indicate that more MCPs and apple domain-containing proteins might be present. EtMIC5 is known to form a multimeric complex with Et-MIC4, a transmembrane protein composed of multiple thrombospondin (TSP)-1 and epidermal growth factor (EGF)-like domains (Periz et al, 2007). In this study, coimmunoprecipitation experiments failed to detect an interaction between EtMIC5 and EtMIC3 or any additional protein other than EtMIC4.…”

Section: Importance Of Sialic Acids In Host Invasion By Coccidiansmentioning

confidence: 47%

Sialic acids: Key determinants for invasion by the Apicomplexa

Friedrich

Matthews

Soldati‐Favre

2010

International Journal for Parasitology

View full text Add to dashboard Cite

a b s t r a c tSialic acids are ubiquitously found on the surface of all vertebrate cells at the extremities of glycan chains and widely exploited by viruses and bacteria to enter host cells. Carbohydrate-bearing receptors are equally important for host cell invasion by the obligate intracellular protozoan parasites of the phylum Apicomplexa. Host cell entry is an active process relying crucially on proteins that engage with receptors on the host cell surface and promote adhesion and internalisation. Assembly into complexes, proteolytic processing and oligomerization are important requirements for the functionality of these adhesins. The combination of adhesive proteins with varying stringency in specificity confers some flexibility to the parasite in face of receptor heterogeneity and immune pressure. Sialic acids are now recognised to critically contribute to selective host cell recognition by various species of the phylum. Ó

show abstract

Section: Importance Of Sialic Acids In Host Invasion By Coccidiansmentioning

confidence: 47%

Sialic acids: Key determinants for invasion by the Apicomplexa

Friedrich

Matthews

Soldati‐Favre

2010

International Journal for Parasitology

View full text Add to dashboard Cite

show abstract

“…Noteworthy, orthologs for most glideosome components were recently also identified in most other apicomplexan parasites (reviewed in [76]), including their recent discovery in Eimeria tenella [77]. The MIC orthologs EtMIC4 and EtMIC5 also aggregate to a multimeric protein complex [78].…”

Section: Multi-protein Complexes Of Malaria Parasitesmentioning

confidence: 98%

Family members stick together: multi-protein complexes of malaria parasites

Kuehn

Simon

Pradel

2010

Med Microbiol Immunol

View full text Add to dashboard Cite

Malaria parasites express a broad repertoire of proteins whose expression is tightly regulated depending on the life-cycle stage of the parasite and the environment of target organs in the respective host. Transmission of malaria parasites from the human to the anopheline mosquito is mediated by intraerythrocytic sexual stages, termed gametocytes, which circulate in the peripheral blood and are essential for the spread of the tropical disease. In Plasmodium falciparum, gametocytes express numerous extracellular proteins with adhesive motifs, which might mediate important interactions during transmission. Among these is a family of six secreted proteins with adhesive modules, termed PfCCp proteins, which are highly conserved throughout the apicomplexan clade. In P. falciparum, the proteins are expressed in the parasitophorous vacuole of gametocytes and are subsequently exposed on the surface of macrogametes during parasite reproduction in the mosquito midgut. One characteristic of the family is a co-dependent expression, such that loss of all six proteins occurs if expression of one member is disrupted via gene knockout. The six PfCCp proteins interact by adhesion domain-mediated binding and thus form complexes on the sexual stage surface having adhesive properties. To date, the PfCCp proteins represent the only protein family of the malaria parasite sexual stages that assembles to multimeric complexes, and only a small number of such protein complexes have so far been identified in other life-cycle stages of the parasite.

show abstract

“…S2A). The microneme proteins EtMIC4 and EtMIC5 of Eimeria tenella form a high molecular weight complex that is also pulled down by lactose chromatography and binds host cells (47). EtMIC5 contains 11 apple domains and by comparison with TgMIC4 we deduce that the seventh apple domain may be a lectin similar to TgMIC4-A5 (supplemental Fig.…”

Section: Overall Arrangement Of Tgmic4 Apple Domains-inmentioning

confidence: 99%

Galactose Recognition by the Apicomplexan Parasite Toxoplasma gondii

Marchant

Cowper

Liu

et al. 2012

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: TgMIC4 is an important microneme effector protein from Toxoplasma gondii. Results: The structure of TgMIC4 together with carbohydrate microarray analyses reveal a broad specificity for galactoseterminating sequences. Conclusion: Lectin activity within the fifth apple domain of TgMIC4 is reminiscent of the mammalian galectin family. Significance: TgMIC4 may contribute to parasite dissemination within the host or down-regulation of the immune response.

show abstract

The Microneme Proteins EtMIC4 and EtMIC5 of Eimeria tenella Form a Novel, Ultra-high Molecular Mass Protein Complex That Binds Target Host Cells

Cited by 29 publications

References 45 publications

Sialic acids: Key determinants for invasion by the Apicomplexa

Sialic acids: Key determinants for invasion by the Apicomplexa

Family members stick together: multi-protein complexes of malaria parasites

Galactose Recognition by the Apicomplexan Parasite Toxoplasma gondii

Contact Info

Product

Resources

About