The microbiota of healthy dogs demonstrates individualized responses to synbiotic supplementation in a randomized controlled trial

Tanprasertsuk, Jirayu; Jha, Aashish R.; Shmalberg, Justin; Jones, Roshonda B.; Perry, Lee M.; Maughan, Heather; Honaker, Ryan W.

doi:10.1186/s42523-021-00098-0

Cited by 8 publications

(21 citation statements)

References 71 publications

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“…All dog owners willingly underwent diet transition regardless of study participation. As previously explained ( Tanprasertsuk et al, 2021 ), since fecal samples were collected non-invasively by the dog owners, no institutional animal ethical review was required.…”

Section: Methodsmentioning

confidence: 99%

“…Dogs were not eligible to participate if they had consumed any prebiotics, probiotics, cultured foods, or antibiotics within the last three months, had a significant change in their diet within the last month, or had surgery within the previous three months. The criteria were similar to those previously described ( Tanprasertsuk et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Stool samples from each dog were collected by owners with Nom Nom Plus Microbiome Testing Kits (NomNomNow, Inc., Nashville, TN, USA) at baseline (day 0) and four weeks after the diet transition (day 28). The kits were previously used in research studies ( Jha et al, 2020 ; Tanprasertsuk et al, 2021 ). All stool samples were processed and sequenced in a single batch at Diversigen, Inc. (New Brighton, MN, USA).…”

Section: Methodsmentioning

confidence: 99%

“…All stool samples were processed and sequenced in a single batch at Diversigen, Inc. (New Brighton, MN, USA). DNA extraction and library construction protocols were performed as previously described ( Johnson et al, 2019 ; Tanprasertsuk et al, 2021 ), with the exception that DNA was extracted using the Zymogen Quick-DNA Fecal/Soil Microbe 96 Mag Bead kit (Zymo Research, Irvine, CA, USA) using Powerbead Pro (Qiagen, Redwood City, CA, USA) plates with 0.5 and 0.1 mm ceramic beads. Extraction controls included a no template control (water) and a characterized homogenized stool.…”

Section: Methodsmentioning

confidence: 99%

“…The non-rarefied count data were log-transformed and principal coordinate analysis (PCoA) was performed using Bray–Curtis dissimilarity calculated with the vegan package at the species level ( Oksanen et al, 2014 ; Tanprasertsuk et al, 2021 ). Using the vegan package, permutational multivariate analysis of variance (PERMANOVA) was performed using Bray–Curtis dissimilarity with 10,000 randomizations by including timepoints to assess the differences in community composition ( Oksanen et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

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Heterogeneity of gut microbial responses in healthy household dogs transitioning from an extruded to a mildly cooked diet

Tanprasertsuk¹,

Shmalberg

Maughan

et al. 2021

PeerJ

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Background The gut microbiota (GM) is associated with canine health and can be impacted by diet. Dog owners in the U.S. have increasingly shown an interest in feeding their dogs a mildly cooked (MC) diet. However, its impact on canine GM and health remains largely unknown. Methods Healthy household dogs were tracked upon switching from various brands of extruded to MC diets for four weeks. A health assessment was completed and stool samples were collected by each owner before (day 0) and after the diet transition (day 28). Shotgun metagenomic sequencing was performed at both time points to characterize the GM. Results Dogs completed the study by either completing the health assessments (n = 31) or providing stool samples at both time points (n = 28). All owners reported either better or no change in overall health at the end of the study (61% and 39%, respectively), and none reported worse overall health. Defecation frequency was also reported to be lower (58%) or about the same (35%). Principal coordinate (PCo) analysis showed a significant shift (p = 0.004) in the β-diversity of the GM upon diet transition (34.2% and 10.3% explained by the first two axes). The abundances of 70 species increased after the diet change (adjusted p < 0.05), 67% and 24% of which belonged to the Lactobacillales and the Enterobacterales orders respectively. The abundances of 28 species decreased (adjusted p < 0.05), 46%, 18%, and 11% of which belonged to the Clostridiales, Bacillales, and Bacteroidales orders, respectively. Lower Lactobacillales and Enterobacterales, and higher Bacteroidales at baseline were associated with a greater shift along the PCo1 axis. Protein content of the baseline diet was correlated with the shift along the PCo1 axis (ρ = 0.67, p = 0.006). Conclusion Owners reported either improvement or no change in health in dogs transitioning from extruded kibble to MC diets for 4 weeks, but this report of health perception requires further exploration in a controlled trial. Diet change also led to a significant shift in the GM profile of healthy dogs. The magnitude of shift was associated with baseline GM and dietary protein, and warrants further examination of individualized responses and personalized nutrition in companion dogs. These results also support future investigation of the impact of a MC diet on health maintenance given its increasing popularity.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Heterogeneity of gut microbial responses in healthy household dogs transitioning from an extruded to a mildly cooked diet

Tanprasertsuk¹,

Shmalberg

Maughan

et al. 2021

PeerJ

Self Cite

View full text Add to dashboard Cite

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Lactobacillus johnsonii CPN23 vis-à-vis Lactobacillus acidophilus NCDC15 Improves Gut Health, Intestinal Morphometry, and Histology in Weaned Wistar Rats

Kumar

Pattanaik

Jadhav

et al. 2023

Probiotics & Antimicro. Prot.

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Fecal microbiome associations with age, body condition score, and stool consistency in domestic cats (Felis catus) living in an animal shelter

Kittson,

Tanprasertsuk,

Burnham

et al. 2023

Preprint

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Background: Few studies delve into the gut microbiome of domestic cats, and many feline-specific studies are hampered by small sample population sizes. We investigated the fecal microbiome as a proxy for gut microbiome within a large shelter cat population (n=288) in Raleigh, NC, USA from February to July 2021. Single stool samples were collected from cats of various age groups that lived in one of four rooms (adoption, n=117; feral, n=113; quarantine, n=37; isolation, n=20; no room, n=1) at the shelter during the sampling period. The fecal microbiome was characterized by shotgun sequencing. Statistical differences in α- and β-diversity were assessed based on assigned shelter room as well as cat age group, body condition score (BCS; scale 1-9), and stool consistency (Bristol fecal scale). Enrichment of composition (as characterized by microbial taxa) and functional potential (as characterized by Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology (KO) terms) based on subject characteristics were evaluated. Results: Analyses consistently indicated significant (p<0.05) differences in β-diversity based on assigned shelter room, age, body condition score, and stool consistency. The β-diversity within samples from cats housed within the adoption room was different from that of the other rooms. In KO terms, samples from the isolation room were also different from the adoption and feral room but not the quarantine room. Regarding age, kittens differed significantly in β-diversity from mature adults in post-hoc comparisons, and mature adults were abundant in much fewer microbiome species and KO terms when compared to kittens and young adults. Underweight and normal weight cats had different microbial profiles, and underweight cats were deficient in a KO term related to microbial lysine biosynthesis. Microbial taxa enriched in felines with overly dense stool or diarrhea were reported, with felines producing dense stools abundant in members of the genera Desulfovibrio and Alistipes and deficient in probiotic Lactobacillus spp. and diarrhetic cats abundant in members of the families Lachnospiraceae and Streptococcaceae. There were no significant differences among α-diversity metrics. Conclusions: To our knowledge, this research utilizes the largest feline microbiome dataset to date and validates previously reported differences in gut microbiome diversity due to age, BCS, and stool consistency while illuminating novel differences associated with the shelter setting. Gaining insight into the diverse microbial populations within the feline gut microbiome is essential for future research into enhancing host health and quality of life.

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The microbiota of healthy dogs demonstrates individualized responses to synbiotic supplementation in a randomized controlled trial

Cited by 8 publications

References 71 publications

Heterogeneity of gut microbial responses in healthy household dogs transitioning from an extruded to a mildly cooked diet

Heterogeneity of gut microbial responses in healthy household dogs transitioning from an extruded to a mildly cooked diet

Lactobacillus johnsonii CPN23 vis-à-vis Lactobacillus acidophilus NCDC15 Improves Gut Health, Intestinal Morphometry, and Histology in Weaned Wistar Rats

Fecal microbiome associations with age, body condition score, and stool consistency in domestic cats (Felis catus) living in an animal shelter

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