The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases

Chen, Chen; Song, Xiaolei; Wei, Weixia; Huang, Zhong; Dai, Jing; Lan, Zhou; Li, Fēi; Yu, Xinlei; Feng, Qiang; Wang, Zirong; Xie, Hailiang; Chen, Xiaomin; Zeng, Chunwei; Wen, Bo; Zeng, Liping; Du, Hui; Tang, Huiru; Xu, Changlu; Xia, Yan; Xia, Huihua; Yang, Huanming; Wang, Jian; Wang, Jun; Madsen, Lise; Brix, Susanne; Kristiansen, Karsten; Xu, Xun; Li, Junhua; Wu, Ruifang; Jia, Huijue

doi:10.1038/s41467-017-00901-0

Cited by 615 publications

(746 citation statements)

References 46 publications

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“…We showed that blood DNAemia in healthy individuals is also associated with the presence of culturable microbiota in the blood. Our results confirm that the blood of healthy humans is not as sterile as previously supposed by other authors [4]- [15] [20]. This work brings evidences, that the resident blood microbiota in healthy individuals should be considered non-pathogenic and a normal feature of the healthy blood.…”

Section: Resultssupporting

confidence: 80%

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Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Panaiotov¹,

Filevski²,

Equestre³

et al. 2018

AiM

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Background: On the analogy of the non-pathogenic microbiota found in oral cavity, skin and gastrointestinal tract, existence of blood microbiota was confirmed by DNA sequencing, but never deeply characterized. Hypothesis for the existence of dormant blood microbiota in healthy humans have been arisen and single species have been isolated. The aim of our study was to resuscitate and investigate the biodiversity of bacterial and fungal dormant blood microbiota in healthy individuals by blood culturing and NGS DNA sequencing. Results: Twenty eight blood samples of healthy individuals, seven for each blood type, were studied. Several culture media were tested. Blood microbiota resuscitation was performed in BHI broth supplemented with vitamin K 1 mg/ml, 2% sucrose, 0.25% sodium citrate and 0.2% yeastolate at 43˚C for 72 h. All tested blood samples were culture positive, as confirmed by Gram staining and TEM. TEM images demonstrated well defined cell structures. Analysis for bacterial and eukaryotic species was performed by 16S rRNA and ITS2 targeted sequencing. The obtained sequences were clustered (≥97% identity) in Operational Taxonomic Units (OTUs). Among cultured and uncultured samples we identified OTUs similarity with 47 bacterial orders belonging to 15 phyla and 39 fungi orders blonging to 2 phyla. For the first time we demonstrated isolation and sequencing identification of fungal blood microbiota in healthy individuals. Blood-group differences were identified among the bacterial microbiome compositions. Conclusion: The dormant blood microbiome is innate of the healthy individuals. Interventional strategies to bind the host blood microbiome with the states of health and disease remain an unmet research goal.

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Section: Resultssupporting

confidence: 80%

“…In a recent study Chen et al (2017) showed that the uterus and the fallopian tubes, which are generally believed to be sterile, are home of rich microbiota indicative of a non-sterile environment [20]. Table 2.…”

Section: Resultsmentioning

confidence: 99%

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Panaiotov¹,

Filevski²,

Equestre³

et al. 2018

AiM

View full text Add to dashboard Cite

show abstract

“…This sterility was thought to be maintained by the cervical mucosal system, which provides an impermeable barrier against bacterial ascension from the vagina . However, this hypothesis was refuted, and recent research has shown microorganisms detected even in the endometrial cavity of healthy asymptomatic women . Furthermore, the uterine mucus plug has been shown to incompletely block bacterial ascension by vaginal bacteria .…”

Section: Etiologymentioning

confidence: 99%

“…Recent research has also shown microorganisms in the peritoneum . It may be possible that peritoneal microorganisms from the gastrointestinal tract reach the uterus via the fallopian tube.…”

Section: Etiologymentioning

confidence: 99%

Review: Chronic endometritis and its effect on reproduction

Kimura

Takebayashi

Ishida

et al. 2019

J of Obstet and Gynaecol

174

134

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Aim Chronic endometritis (CE) is a disease of continuous and subtle inflammation characterized by the infiltration of plasma cells in the endometrial stromal area. Although the clinical significance of CE has been thought in clinical practice for a long time because it is either asymptomatic or presents with subtle symptoms, recent studies have shown the potential adverse effects of CE on fertility. In the present review, we focus on the concept, diagnosis, etiology, pathophysiology, diagnosis, impact on reproduction and treatment for it to understand CE. Methods The published articles were reviewed. Results The prevalence of CE has been found to be 2.8–56.8% in infertile women, 14–67.5% in women with recurrent implantation failure (RIF), and 9.3–67.6% in women with recurrent pregnancy loss. Microorganisms are thought to be a main cause of CE, since antibiotic treatment has been reported to be an effective therapy for CE. Common bacteria are frequently detected in the uterine cavity of CE patients by microbial culture. In CE endometrium, the prevalence of immune cells and decidualization has been reported to be modified, and these modifications are thought to adversely affect fertility. The gold standard for the diagnosis of CE is the histological detection of plasma cells in the stromal area of the endometrium in endometrial specimens, although universally accepted criteria for the diagnosis of CE have not been determined. The treatment currently thought to be most effective for the recovery of fertility in CE is administration of oral antibiotics. Patients whose CE has been cured have been reported to have a higher ongoing pregnancy rate, clinical pregnancy rate, and implantation rate compared with patients with persistent CE. Conclusion CE greatly affects implantation and impairs fertility. Antibiotic administration is an effective therapeutic option. Pregnancy rate in in vitro fertilization is improved when CE is cured by antibiotic.

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“…The immune system within each region is independently regulated by cyclical changes in the local endocrine environment. [5][6][7] Although a degree of skepticism toward these results exists, 8 the application of similar experimental methodologies to the uteri of other species has also identified similar low biomass bacterial colonization. While the lower reproductive tract contains a microbiome dominated by lactobacilli, which is thought to play a key role in normal function, 4 the presence of bacteria within the upper reproductive tract is typically associated with disease.…”

Section: Introductionmentioning

confidence: 99%

Intrauterine vaccination induces a dose‐sensitive primary humoral response with limited evidence of recall potential

Pasternak

Hamonic

Kessel

et al. 2018

American J Rep Immunol

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The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases

Cited by 615 publications

References 46 publications

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Review: Chronic endometritis and its effect on reproduction

Intrauterine vaccination induces a dose‐sensitive primary humoral response with limited evidence of recall potential

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