The Microbiota and It’s Correlation With Metabolites in the Gut of Mice With Nonalcoholic Fatty Liver Disease

Abstract

In recent years, nonalcoholic fatty liver disease (NAFLD) has become the most common liver disease in the world. As an important model animal, the characteristics of gut microbiota alteration in mice with NAFLD have been studied but the changes in metabolite abundance in NAFLD mice and how the gut microbiota affects these intestinal metabolites remain unclear. In this experiment, a mouse model for NAFLD was established by a high-fat diet. The use of 16S rDNA technology showed that while there were no significant changes in the alpha diversity in the cecum of NAFLD mice, the beta diversity changed significantly. The abundance of Blautia, Unidentified-Lachnospiraceae, Romboutsia, Faecalibaculum, and Ileibacterium increased significantly in NAFLD mice, while Allobaculum and Enterorhabdus decreased significantly. Amino acids, lipids, bile acids and nucleotide metabolites were among the 167 significantly different metabolites selected. The metabolic pathways of amino acids, SFAs, and bile acids were significantly enhanced, while the metabolic pathways of PUFAs, vitamins, and nucleotides were significantly inhibited. Through correlation and MIMOSA2 analysis, it is suggested that gut microbiota does not affect the changes of lipids and bile acids but can reduce thiamine, pyridoxine, and promote L-phenylalanine and tyramine production. The findings of this study will help us to better understand the relationship between gut microbiota and metabolites in NAFLD.

Qingrequzhuo capsule alleviated methionine and choline deficient diet-induced nonalcoholic steatohepatitis in mice through regulating gut microbiota, enhancing gut tight junction and inhibiting the activation of TLR4/NF-κB signaling pathway

“…Similar results were obtained in this study that Muribaculaceae had wide and negative correlations with therapeutic indicators. The abundance of Faecalibaculum is known to be significantly elevated in NAFLD mice, which was consistent with our results ( 56 , 57 ). Positive correlations were also found between Faecalibaculum and most therapeutic indicators.…”

Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet

“…Compared with the results of human related studies, was found that in the colon, human and mice have similar bacterial species at the phylum and family level, but have differences at the genus level, which is likely caused by host species. Two studies on cecal microbiota in mice showed a significant increase in the abundance of Faecalibaculum in cecal microbiota in NAFLD mice compared with normal mice (Mu et al, 2020;Gu et al, 2022), which was similar to our results.…”

“…In NAFLD mice, at the genus level, five biomarkers in the cecum of NAFLD mice, Blautia , Desulfovibrio , Faecalibaculum , Helicobacter , and unidentified Lachnospiraceae , were also the biomarkers in the colon. Interestingly, in a previous study, Blautia , Faecalibaculum , Helicobacter and unidentified Lachnospiraceae are significantly increased in NAFLD mice ( Gu et al, 2022 ). However, the ileum and large intestine (cecum and colon) shared only a few biomarkers, with only one common biomarker at the genus level.…”

16S rRNA and Metagenomics Combined with UPLC-Q/TOF-MS Metabolomics Analysis Reveals the Potential Mechanism of Radix Astragali Against Hyperuricemia in Mice

“… 26 , 27 Ileibacterium and Peptococcaceae have been reported to be positively correlated with metabolic diseases. 28 , 29 Next, at the species level, in line with our heatmap of correlation observation, the relative abundances of Lactobacillus murine, Lachnospiraceae_bacterium_MD335 and Lachnospiraceae_bacterium_A4 were increased after RA intervention compared with untreated mice. In addition, the correlation analysis suggested that murine Lactobacillus was involved in inhibiting XOD activity in serum and improving liver and kidney functions.…”