The Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stage

Oresta, Bianca; Braga, Daniele; Lazzeri, Massimo; Frego, Nicola; Saita, Alberto; Faccani, Cristina; Fasulo, Vittorio; Colombo, Piergiuseppe; Guazzoni, Giorgio; Hurle, Rodolfo; Rescigno, María

doi:10.1097/ju.0000000000001336

Cited by 45 publications

(48 citation statements)

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“…Various microbial communities and urotypes are reported to be associated with non-infectious disorders and conditions [25], such as bladder cancer [27,51], prostatic neoplasia [24], or urinary incontinence [24,27]. We report significantly different β-diversity in A. schaalii-positive and -negative samples (defined by NGS, see Supplementary Table S8) and patients (defined by PCR-DGGE-S and culture, see Chapter 3.1.3).…”

Section: Discussionmentioning

confidence: 71%

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

et al. 2021

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Actinotignum schaalii is an emerging, opportunistic pathogen and its connection to non-infectious diseases and conditions, such as prostate or bladder cancer, or chronic inflammation has been proposed. Here, we analyzed 297 urine, ureteral and urinary catheter samples from 128 patients by Polymerase Chain Reaction followed by Denaturing Gradient Gel Electrophoresis and Sequencing (PCR-DGGE-S), and culture, and 29 of these samples also by 16S rRNA Illumina sequencing, to establish A. schaalii’s prevalence in urinary tract-related samples, its relation to other bacteria, and its potential association with patients’ conditions and samples’ characteristics. A. schaalii-positive samples were significantly more diverse than A. schaalii negative and between-group diversity was higher than intra-group. Propionimicrobium lymphophilum, Fusobacterium nucleatum, Veillonella sp., Morganella sp., and Aerococcus sp. were significantly more often present in A. schaalii-positive samples; thus, we suggest these species are A. schaalii’s concomitants, while Enterobacter and Staphylococcaceae were more often identified in A. schaalii-negative samples; therefore, we propose A. schaalii and these species are mutually exclusive. Additionally, a significantly higher A. schaalii prevalence in patients with ureter stricture associated hydronephrosis (p = 0.020) was noted. We suggest that A. schaalii could be an early polybacterial biofilm colonizer, together with concomitant species, known for pro-inflammatory features.

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Section: Discussionmentioning

confidence: 71%

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

et al. 2021

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“…Eine andere Arbeit konnte die besagten Unterschiede nicht bestätigen [25]. Unter den Studien mit positiven Ergebnissen spielte unter anderem das Geschlecht [26] sowie die Art der Probengewinnung eine wesentliche Rolle [27,28]. Hier wurde bei der Mikrobiomuntersuchung zwischen Mittelstrahlurin, Katheterurin und Biopsie unterschieden.…”

Section: Nierenzellkarzinom Und Harnblasenkarzinomunclassified

Die Rolle des Mikrobioms bei urologischen Erkrankungen

et al. 2021

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ZusammenfassungDer Einsatz moderner molekularer Technologien gab uns im letzten Jahrzehnt neue Einblicke in die komplexen Interaktionen des menschlichen Mikrobioms in Bezug auf Gesundheit und Krankheitsentstehung. Hierbei wurde unter anderem das Sterilitätskonzept des Harntrakts verworfen, sodass man sich nun als Ziel gesetzt hat, die verschiedenen mikrobiellen Signaturen zu identifizieren, die mit diversen Erkrankungen assoziiert sind. So stehen Dysbalancen des Mikrobioms zunehmend im Verdacht, negative Auswirkungen auf diverse maligne und benigne Krankheitsbilder zu verursachen. Kürzlich konnten solche Zusammenhänge auch für das Prostatakarzinom, Nierenzellkarzinom und Harnblasenkarzinom gezeigt werden. Das Ergebnis hiervon ist die Entdeckung potenziell neuer Biomarker zur Diagnostik und als Therapieziel der genannten Erkrankungen. Für die Diagnostik einiger benigner Krankheitsbilder wie der interstitiellen Zystitis, der Dranginkontinenz und der chronischen Prostatitis bzw. dem chronischen Beckenschmerzsyndrom galt eine mikrobielle Beteiligung bislang als Ausschlusskriterium. Jedoch zeigen auch hier die aktuellen Studien, dass das patientenindividuelle Mikrobiom durchaus Einfluss auf Entstehung und Ausprägung der jeweiligen Erkrankung haben kann.

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“…Table 2). Based on the compositional differences we observed by collection method and on other studies that have reported differences in urine microbiota due to collection method [41,[61][62][63][64][65], we opted to limit the remainder of our analyses to samples collected via free catch only. This allowed us to compare microbiota in urine from healthy dogs and dogs with UC without introducing collection method as a potential confounder.…”

Section: Sample Collectionmentioning

confidence: 99%

“…In dogs with UC, a variety of urine collection methods were employed as deemed clinically appropriate including: midstream free catch, catheter, or cystoscopy. Free catch urine can include bacteria from the bladder, urethra, periurethral skin, prepuce, or vagina, while urine collected via catheterization or cystoscopy primarily includes microbes from the bladder and limits the presence of genital and skin microbes [41,[61][62][63]. To determine if collection method could potentially influence our results, we compared samples from dogs with UC collected via free catch (n = 8) to samples collected via non-free catch methods (catheterization, cystoscopy) (n = 11) (Supp.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Alterations in urine microbiota have been reported in association with multiple genitourinary diseases including chronic kidney disease [25], chronic prostatitis, chronic pelvic pain syndrome [26], interstitial cystitis [27], sexually transmitted infections [28], urgency urinary incontinence [29], urinary tract infections [30], urinary stone disease [31], urogenital schistosomiasis [32], urogynecologic surgery [33], and vaginosis [34]. A few recent studies on the urine / bladder microbiome have also revealed subtle but intriguing differences in urine or bladder tissue microbial diversity and composition of individuals with and without UC (Table 1) [17,[35][36][37][38][39][40][41][42][43][44][45], but approaches and results in these studies vary widely. Studies in relevant animal models could advance this research by offering a more controlled environment.…”

Section: Introductionmentioning

confidence: 99%

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Urine and Fecal Microbiota in a Canine Model of Bladder Cancer

Mrofchak

Madden

Evans

et al. 2021

Preprint

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Introduction: Urothelial carcinoma (UC) is the tenth most diagnosed cancer in humans worldwide. Dogs are a robust model for invasive UC as tumor development and progression is similar in humans and dogs. Recent studies on urine microbiota in humans revealed alterations in microbial diversity and composition in individuals with UC; however, the potential role of microbiota in UC has yet to be elucidated. Dogs could be valuable models for this research, but microbial alterations in dogs with UC have not been evaluated. Objective: The objective of this this pilot study was to compare the urine and fecal microbiota of dogs with UC (n = 7) and age-, sex-, and breed-matched healthy controls (n = 7). Methods: DNA was extracted from mid-stream free-catch urine and fecal samples using Qiagen Bacteremia and PowerFecal kits, respectively. 16S rRNA gene sequencing was performed followed by sequence processing and analyses (QIIME 2 and R). Results: Canine urine and fecal samples were dominated by taxa similar to those found in humans. Significantly decreased microbial diversity (Kruskal-Wallis: Shannon, p = 0.048) and altered bacterial composition were observed in the urine but not feces of dogs with UC (PERMANOVA: Unweighted UniFrac, p = 0.011). The relative abundances of Fusobacterium was also increased, although not significantly, in the urine and feces of dogs with UC. Conclusion: This study characterizes urine and fecal microbiota in dogs with UC, and it provides a foundation for future work exploring host-microbe dynamics in UC carcinogenesis, prognosis, and treatment.

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The Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stage

Cited by 45 publications

References 2 publications

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

Actinotignum schaalii: Relation to Concomitants and Connection to Patients’ Conditions in Polymicrobial Biofilms of Urinary Tract Catheters and Urines

Die Rolle des Mikrobioms bei urologischen Erkrankungen

Urine and Fecal Microbiota in a Canine Model of Bladder Cancer

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