The microbial flora of Aphis gossypii: Patterns across host plants and geographical space

Najar-Rodríguez, Adriana J.; McGraw, Elizabeth A.; Mensah, Robert; Pittman, Geoffrey W.; Walter, Gimme H.

doi:10.1016/j.jip.2008.10.005

Cited by 36 publications

(39 citation statements)

References 33 publications

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“…Alternatively, differences between islands may be due to stochastic forces, such as ecological drift, acting on communities of aphid-associated bacteria (14). Bacterial communities did not differ based on the host plant (Table 2), and our results corroborate the finding that geography has a greater effect on bacterial community assemblages than plant use in A. gossypii (24). This result is somewhat unexpected in light of previous work that found facultative symbionts to increase aphid fitness on certain plants (17,32).…”

Section: Resultssupporting

confidence: 81%

“…However, only scattered information is available on the bacteria associated with other aphid species (13,24), and little is known about the entire bacterial communities of aphids. Highthroughput DNA sequencing approaches have opened up new venues to characterize bacterial communities, and this approach permits the investigation of ecological questions that are not possible to address with more traditional microbial genotyping procedures (2,3,8,18).…”

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confidence: 99%

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Bacterial Communities of Two Parthenogenetic Aphid Species Cocolonizing Two Host Plants across the Hawaiian Islands

Jones

Bressan

Greenwell

et al. 2011

Appl Environ Microbiol

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Aphids (Hemiptera: Aphididae) have been the focus of several studies with respect to their interactions with inherited symbionts, but bacterial communities of most aphid species are still poorly characterized. In this research, we used bar-coded pyrosequencing to characterize bacterial communities in aphids. Specifically, we examined the diversity of bacteria in two obligately parthenogenetic aphid species (the melon aphid, Aphis gossypii, and the cardamom aphid, Pentalonia caladii) cocolonizing two plant species (taro, Colocasia esculenta, and ginger, Alpinia purpurata) across four Hawaiian Islands (Hawaii, Kauai, Maui, and Oahu). Results from this study revealed that heritable symbionts dominated the bacterial communities for both aphid species. The bacterial communities differed significantly between the two species, and A. gossypii harbored a more diverse bacterial community than P. caladii. The bacterial communities also differed across aphid populations sampled from the different islands; however, communities did not differ between aphids collected from the two host plants.

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Section: Resultssupporting

confidence: 81%

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confidence: 99%

Bacterial Communities of Two Parthenogenetic Aphid Species Cocolonizing Two Host Plants across the Hawaiian Islands

Jones

Bressan

Greenwell

et al. 2011

Appl Environ Microbiol

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“…Eight bacterial groups, including Arsenophonus , Serratia , Rickettsia , and Wolbachia were cocolonized on Colocasia esculenta and Alpinia purpurata across the four Hawaiian Islands (Jones, Bressan, Greenwell, & Fierer, ). In this study, the relative abundances of 22 identified genera were more than 0.1%, including the three most abundant bacterial genera Arsenophonus , Hamiltonella and Acinetobacter and the three genera with low abundance ( Pseudomonas , Burkholderia , Stenotrophomonas ) that have been reported in A. gossypii (Jones et al., ; Najar‐Rodriguez et al., ; Zhao et al., ). None of the aphids surveyed were infected with the main endosymbiotic types ( Serratia symbiotica , Regiella insecticola , Rickettsia , Spiroplasma ) that were previously reported for other aphid species (Russell, Latorre, Sabater‐Munoz, Moya, & Moran, ).…”

Section: Discussionmentioning

confidence: 48%

“…(Fukatsu et al., ; Guay, Boudreault, Michaud, & Cloutier, ; Sakurai, Koga, Tsuchida, Meng, & Fukatsu, ; Tsuchida, Koga, Fujiwara, & Fukatsu, ). In DGGE, 5 bands closely related to Arsenophonus , Pseudomonas , Acinetobacter , Pelomonas, and Burkholderia were separated from malvaceous and cucurbitaceous populations within Japan and Australia (Najar‐Rodriguez, McGraw, Mensah, Pittman, & Walter, ). Eight bacterial groups, including Arsenophonus , Serratia , Rickettsia , and Wolbachia were cocolonized on Colocasia esculenta and Alpinia purpurata across the four Hawaiian Islands (Jones, Bressan, Greenwell, & Fierer, ).…”

Section: Discussionmentioning

confidence: 99%

Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China

et al. 2018

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The cotton-melon aphid, Aphis gossypii Glover, is a worldwide-spreading species, and pesticide-resistant populations are increasing rapidly. In this study, investigations were performed based on Illumina HiSeq sequencing of the 16S rDNA V4 region for the bacterial communities embodied as intracellular symbionts under natural and in pesticide-treated populations of A. gossypii. The results revealed that more than 82% of bacterial communities belonged to the phylum Proteobacteria in which the maximum proportion (53.24%) was of the genus Arsenophonus; Hamiltonella composed 22.31; and 1.37% was of the genus Acinetobacter. The relative abundance of Hamiltonella was obvious, vertically transmitted, divided into two groups, and its infection influenced the bacterial communities in A. gossypii. Symbiont density and composition were changed in samples tested on different days. Azadirachtin and phoxim influenced on the composition of bacterial communities. Different biomarkers were used for pesticide-treated samples with LEfSe results. These findings will increase awareness regarding bacterial communities in naturally occurring populations of A. gossypii and pave the way to study the relationship between symbionts and pesticide resistance.

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“…This aphid harbors the obligate endosymbiont Buchnera, while the facultative bacteria found commonly in the pea aphid and related species, such as Regiella insecticola, H. defensa, and S. symbiotica, are absent or rare (32)(33)(34). The taxon Aphis gossypii (like the pea aphid) is composed of a series of host plant races or biotypes that are to various degrees specialized on different host plant groups.…”

mentioning

confidence: 99%

Host Plant Determines the Population Size of an Obligate Symbiont (Buchnera aphidicola) in Aphids

Zhang

Cao

Zhong

et al. 2016

Appl Environ Microbiol

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bBuchnera aphidicola is an obligate endosymbiont that provides aphids with several essential nutrients. Though much is known about aphid-Buchnera interactions, the effect of the host plant on Buchnera population size remains unclear. Here we used quantitative PCR (qPCR) techniques to explore the effects of the host plant on Buchnera densities in the cotton-melon aphid, Aphis gossypii. Buchnera titers were significantly higher in populations that had been reared on cucumber for over 10 years than in populations maintained on cotton for a similar length of time. Aphids collected in the wild from hibiscus and zucchini harbored more Buchnera symbionts than those collected from cucumber and cotton. The effect of aphid genotype on the population size of Buchnera depended on the host plant upon which they fed. When aphids from populations maintained on cucumber or cotton were transferred to novel host plants, host survival and Buchnera population size fluctuated markedly for the first two generations before becoming relatively stable in the third and later generations. Host plant extracts from cucumber, pumpkin, zucchini, and cowpea added to artificial diets led to a significant increase in Buchnera titers in the aphids from the population reared on cotton, while plant extracts from cotton and zucchini led to a decrease in Buchnera titers in the aphids reared on cucumber. Gossypol, a secondary metabolite from cotton, suppressed Buchnera populations in populations from both cotton and cucumber, while cucurbitacin from cucurbit plants led to higher densities. Together, the results suggest that host plants influence Buchnera population processes and that this may provide phenotypic plasticity in host plant use for clonal aphids.A phids harbor two types of bacterial endosymbionts: the obligate symbiont Buchnera aphidicola, which occurs in almost all aphids, and facultative bacteria belonging to a variety of taxa that are not essential for aphid survival and typically are found in some but not all individuals of a population (1-3). The association between Buchnera and aphids was established 80 to 150 million years ago (4), and because transmission is strictly maternal, bacteria and host phylogenies are perfectly congruent. Buchnera is essential for the aphid, and if the symbiont is experimentally removed, the host grows very slowly and cannot reproduce (5-7). Buchnera synthesizes essential amino acids and other substances that are absent in the aphid's phloem diet (1,(8)(9)(10)(11)(12)(13) and is active in purine metabolism (14, 15). The symbiont's activities can be disrupted by heat shock, and it has been shown that variation in the promoter of a Buchnera heat shock gene impacts aphid thermal tolerance (16,17). The genome of Buchnera is much smaller than those of its free-living relatives (18, 19) and in many ways it now resembles an organelle, highly integrated within the physiology of its host.Densities of Buchnera (as measured by quantitative PCR [qPCR] relative to the host) are affected by host age and genotype and by ...

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The microbial flora of Aphis gossypii: Patterns across host plants and geographical space

Cited by 36 publications

References 33 publications

Bacterial Communities of Two Parthenogenetic Aphid Species Cocolonizing Two Host Plants across the Hawaiian Islands

Bacterial Communities of Two Parthenogenetic Aphid Species Cocolonizing Two Host Plants across the Hawaiian Islands

Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China

Host Plant Determines the Population Size of an Obligate Symbiont (Buchnera aphidicola) in Aphids

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