The microbial electrochemical Peltier heat: an energetic burden and engineering chance for primary microbial electrochemical technologies

Korth, Benjamin; Maskow, Thomas; Picioreanu, Cristian

doi:10.1039/c6ee01428c

Cited by 18 publications

(10 citation statements)

References 30 publications

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“…Nevertheless, the microbial window of opportunities is firstly defined by thermodynamics and its utilization by the other factors or in metaphoric terms: “ Thermodynamics sets the frame, evolution draws the picture ” (Schoepp-Cothenet et al, 2013). So far, energetic assessments of EAM were each restricted to single aspects of thermodynamics: either quantification of biomass production, Gibbs free energy calculations (only valid with corrections for non-standard conditions), or measuring of heat production (Mahadevan et al, 2006; Schröder, 2007; Marsili et al, 2010; Korth et al, 2015, 2016). For an overall thermodynamic analysis, these isolated aspects need to be linked and measured in the same condition (in best case scenario in one set of experiments).…”

Section: Introductionmentioning

confidence: 99%

Spotlight on the Energy Harvest of Electroactive Microorganisms: The Impact of the Applied Anode Potential

Korth

2019

Front. Microbiol.

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Electroactive microorganisms (EAM) harvest energy by reducing insoluble terminal electron acceptors (TEA) including electrodes via extracellular electron transfer (EET). Therefore, compared to microorganisms respiring soluble TEA, an adapted approach is required for thermodynamic analyses. In EAM, the thermodynamic frame (i.e., maximum available energy) is restricted as only a share of the energy difference between electron donor and TEA is exploited via the electron-transport chain to generate proton-motive force being subsequently utilized for ATP synthesis. However, according to a common misconception, the anode potential is suggested to co-determine the thermodynamic frame of EAM. By comparing the model organism Geobacter spp. and microorganisms respiring soluble TEA, we reason that a considerable part of the electron-transport chain of EAM performing direct EET does not contribute to the build-up of proton-motive force and thus, the anode potential does not co-determine the thermodynamic frame. Furthermore, using a modeling platform demonstrates that the influence of anode potential on energy harvest is solely a kinetic effect. When facing low anode potentials, NADH is accumulating due to a slow direct EET rate leading to a restricted exploitation of the thermodynamic frame. For anode potentials ≥ 0.2 V (vs. SHE), EET kinetics, NAD + /NADH ratio as well as exploitation of the thermodynamic frame are maximized, and a further potential increase does not result in higher energy harvest. Considering the limited influence of the anode potential on energy harvest of EAM is a prerequisite to improve thermodynamic analyses, microbial resource mining, and to transfer microbial electrochemical technologies (MET) into practice.

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Section: Introductionmentioning

confidence: 99%

Spotlight on the Energy Harvest of Electroactive Microorganisms: The Impact of the Applied Anode Potential

Korth

2019

Front. Microbiol.

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“…However, this method suffers from two main limitations: (i) this method implicitly assumes that all the redox gradient available to each species is coupled to energy conservation, which may not be the case in reality [27][28][29] and (ii) Heijnen's correlation is only valid for non-inhibitory conditions and does not account for cell maintenance or biological regulations that would reduce growth yields [20]. Thus, this method will provide maximum growth yield and is more likely to provide fair electron mediator redox potential for syntrophic rather than non-mutualistic IET [30].…”

Section: Processmentioning

confidence: 99%

Modeling of interspecies electron transfer in anaerobic microbial communities

Quéméner

Moscoviz

Bernet

et al. 2021

Current Opinion in Biotechnology

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Interspecies electron transfer (IET) is a key phenomenon in anaerobic ecosystems, which is traditionally modelled as hydrogen transfer. Recently discovered alternative mediated IET (MIET) or direct IET (DIET) offer exciting alternative mechanisms of microbial partnerships that could lead to new strategies for the improvement of biotechnologies. Here, we analyze mathematical modelling of DIET and MIET in anaerobic ecosystems. Bioenergetics approaches already enable the evaluation of different energy sharing scenarios between microorganisms and give interesting clues on redox mediators and on possible ways of driving microbial communities relying on IET.The modeling of DIET kinetics however is currently only in its infancy. Recent concepts introduced for the modeling of electroactive biofilms should be further exploited. Recent modeling examples confirms the potential of DIET to increase the IET rates compared to H2-MIET, but also point out the need for additional characterizations of biological components supporting IET to improve predictions. 42.de Lichtervelde ACL, ter Heijne A, Hamelers HVM, Biesheuvel PM, Dykstra JE: Theory of Ion and Electron Transport Coupled with Biochemical Conversions in an Electroactive Biofilm.

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“…As the difference in redox potentials between electron donor and acceptor determines the maximum microbial energy gain attainable through respiration, different anode potentials will result in different energy conservation opportunities for electroactive microorganisms. The net share of energy that can be utilized depends on the formal potential of the donor and that of the final electron transfer site accepting electrons (36). Here we test the hypothesis that particular proteins used within the electron transport pathway of S. oneidensis will alter to maximise energy conservation when the organism is exposed to different anode potentials.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Effect of the anode potential on the physiology and proteome of Shewanella oneidensis MR-1

Grobbler

Virdis

Nouwens

et al. 2018

Bioelectrochemistry

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Shewanella species respire using iron and manganese oxides as well as electrodes as solid terminal electron acceptors. Shewanella oneidenis MR-1 exploits mediated as well as direct extracellular electron transfer (EET) modes to transfer electrons at different formal potentials. These different EET modes at different potentials may utilise alternate electron transfer pathways. Therefore, we investigated how different anode potentials, providing different maximum microbial energy gains impacted S. oneidensis microbial physiology. Using quantitative proteomics, comparative analysis of the cellular variations to different anode potentials was performed. A label-free proteomic mass spectrometric analysis method, SWATH-MS, was used to gather quantitative information to determine physiological changes of Shewanella oneidensis MR-1 grown at different anodic potentials. S. oneidensis was cultured and grown in electrochemical cells at the set anode potentials of +0.71V, +0.21V & -0.19V versus SHE reference electrode, while the current production was monitored. At maximum current, electrodes were removed and whole-cell proteins extracted. Subsequent SWATH-MS analysis revealed information on 740 identified proteins across the three electrode potentials. For the first time, we show the abundance of S. oneidensis electron transfer proteins differs with electrode potential.

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The microbial electrochemical Peltier heat: an energetic burden and engineering chance for primary microbial electrochemical technologies

Abstract: Microbes shoveling electrons heat up: combining calorimetry with microbial electrochemistry allows deciphering heat fluxes of electroactive microorganisms.

Cited by 18 publications

References 30 publications

Spotlight on the Energy Harvest of Electroactive Microorganisms: The Impact of the Applied Anode Potential

Spotlight on the Energy Harvest of Electroactive Microorganisms: The Impact of the Applied Anode Potential

Modeling of interspecies electron transfer in anaerobic microbial communities

Effect of the anode potential on the physiology and proteome of Shewanella oneidensis MR-1

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