The microbial cycling of phosphorus on long-term fertilized soil: Insights from phosphate oxygen isotope ratios

Bi, Qincheng; Zheng, Bang-Xiao; Lin, Xianyong; Li, Kejie; Liu, Xipeng; Hao, Xiu-Li; Zhang, Han; Zhang, Jiabao; Jaisi, Deb P.; Zhu, Yan

doi:10.1016/j.chemgeo.2018.02.013

Cited by 38 publications

(26 citation statements)

References 45 publications

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“…Phosphate oxygen isotope values (δ 18 O P ) have been applied to advance mechanistic understanding of P cycling as well as tracing sources in a variety of environments such as agricultural soils, freshwaters, and marine systems (Angert et al, ; Bauke et al, ; Bi et al, ; Davies et al, ; Gooddy et al, ; Joshi et al, , ; Hacker et al, ; Tamburini et al, ; Siebers et al, ; Tonderski et al, ). In a biological system, oxygen isotope exchange between water and phosphate is rapid and results in isotope equilibrium, which is determined by the ambient water oxygen isotope values (δ 18 O w ) and temperature (Blake et al, ; Longinelli & Nuti, ).…”

Section: Introductionmentioning

confidence: 99%

Loading and Bioavailability of Colloidal Phosphorus in the Estuarine Gradient of the Deer Creek‐Susquehanna River Transect in the Chesapeake Bay

Yuan

Hui

et al. 2019

JGR Biogeosciences

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Phosphorus (P) overloading is a major cause of surface water eutrophication and bottom water hypoxia. The incomplete understanding of different P pools and their corresponding bioavailability in the continuum from sources and sinks has limited the development of appropriate nutrient management strategies. Here we apply multistable isotope proxies to track colloids and identify whether specific P pools in colloids are biologically cycled at the Deer Creek‐Susquehanna River mouth stretch. Results showed that NaOH‐Pi is the most dominant P pool in the summer and winter seasons. Oxygen isotope values (δ18OP) of NaOH‐Pi and HNO3‐Pi pools of different size fractions of colloids are much heavier than the ranges of equilibrium values in the ambient water, which suggest that these two pools are recalcitrant against biological uptake. It further means isotopic signatures of these P pools could be used to identify the sources of colloids. Carbon (C) and nitrogen (N) isotope compositions of colloids showed that the contribution of terrestrial sources gradually decreases downstream of the river toward the bay and Deer Creek contributes disproportionately high amounts of colloids to the Susquehanna River. These findings provide valuable information on the loading of colloids and relative bioavailability of colloidal P pools in estuarine ecosystems.

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Section: Introductionmentioning

confidence: 99%

Loading and Bioavailability of Colloidal Phosphorus in the Estuarine Gradient of the Deer Creek‐Susquehanna River Transect in the Chesapeake Bay

Yuan

Hui

et al. 2019

JGR Biogeosciences

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“…In long‐term field experiments, P fertilization without N supply resulted in higher total and resin P stocks compared to full fertilization ( e.g ., Bauke et al., 2018; Pfahler et al., 2020), as plants were unable to efficiently utilize P resources in the soil when N was lacking. However, N‐limitation did not result in conclusively higher or lower δ 18 O P values of stable P when compared across different sites ( Bauke et al., 2018; Bi et al., 2018; Pfahler et al., 2020). Thus, a greater number of studies than currently available may be required to disentangle the interactive effects of water and N (or other nutrients) supply on P recycling activities as indicated by δ 18 O P values.…”

Section: δ18opin Soilsmentioning

confidence: 93%

“…(2010b) and NaHCO 3 ‐extractable P of Bi et al. (2018)]. Assuming that low P availability induces high levels of P recycling, values close to equilibrium such as those observed in the HCl‐extract would be expected.…”

Section: δ18opin Soilsmentioning

confidence: 94%

Perspectives from the Fritz‐Scheffer Awardee 2019. Oxygen isotopes in phosphate—The key to phosphorus tracing?

Bauke

2020

J. Plant Nutr. Soil Sci.

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Phosphorus (P) tracing in natural environments is challenging, lacking stable P isotopes Oxygen isotope ratios in phosphate (δ18OP) represent a novel tool for tracing the biological cycling of P from the global scale down to hotspots at the micro‐scale and within particular soil compartments such as aggregates or pores. Despite the small number of studies available so far, existing data indicate that δ18OP values point to where, at what extent and how efficiently P is recycled in soils.

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“…In contrast, P concentrations in sodium bicarbonate extracts are usually greater than in water extracts, but the high solution pH, carbonate and salt concentration could lead to problems during the purification of P for δ 18 O P determination. Degassing prior to the purification and precipitation of brucite is recommended to further clean the extracts . In addition, sodium bicarbonate extracts are slightly alkaline, and can therefore extract a considerable amount of organic P. The purification protocol for the δ 18 O P determination only targets inorganic P, but extracted organic P could be hydrolysed under the acidic conditions of the colorimetric assay of orthophosphate .…”

Section: Introductionmentioning

confidence: 99%

“…Degassing prior to the purification and precipitation of brucite is recommended to further clean the extracts. 19,20 In addition, sodium bicarbonate extracts are slightly alkaline, and can therefore extract a considerable amount of organic P. The purification protocol for the δ 18 O P determination only targets inorganic P, but extracted organic P could be hydrolysed under the acidic conditions of the colorimetric assay of orthophosphate. 21 As the orthophosphate concentrations are used to calculate the δ 18 O P values of microbial P, hydrolysis of organic P might lead to erroneous results.…”

Section: Introductionmentioning

confidence: 99%

A rapid ammonium fluoride method to determine the oxygen isotope ratio of available phosphorus in tropical soils

Pfahler

Bielnicka

Smith

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale The isotopic composition of oxygen bound to phosphorus (δ18OP value) offers an opportunity to gain insight into P cycling mechanisms. However, there is little information for tropical forest soils, which presents a challenge for δ18OP measurements due to low available P concentrations. Here we report the use of a rapid ammonium fluoride extraction method (Bray‐1) as an alternative to the widely used anion‐exchange membrane (AEM) method for quantification of δ18OP values of available P in tropical forest soils. Methods We compared P concentrations and δ18OP values of available and microbial P determined by AEM and Bray‐1 extraction for a series of tropical forest soils from Panama spanning a steep P gradient. This involved an assessment of the influence of extraction conditions, including temperature, extraction time, fumigation time and solution‐to‐soil ratio, on P concentrations and isotope ratios. Results Depending on the extraction conditions, Bray‐1 P concentrations ranged from 0.2 to 66.3 mg P kg−1 across the soils. Extraction time and temperature had only minor effects on Bray‐1 P, but concentrations increased markedly as the solution‐to‐soil ratio increased. In contrast, extraction conditions did not affect Bray‐1 δ18OP values, indicating that Bray‐1 provides a robust measure of the isotopic composition of available soil P. For a relatively high P soil, available and fumigation‐released (microbial) δ18OP values determined by Bray‐1 extraction (20‰ and 16‰, respectively) were higher than those determined by the AEM method (18‰ and 12‰, respectively), which we attribute to slightly different P pools extracted by the two methods and/or differences resulting from the longer extraction time needed for the AEM method. Conclusions The short extraction time, insensitivity to extraction conditions and smaller mass of soil required to extract sufficient P for isotopic analysis make Bray‐1extraction a suitable alternative to the AEM method for the determination of δ18OP values of available P in tropical soils.

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The microbial cycling of phosphorus on long-term fertilized soil: Insights from phosphate oxygen isotope ratios

Cited by 38 publications

References 45 publications

Loading and Bioavailability of Colloidal Phosphorus in the Estuarine Gradient of the Deer Creek‐Susquehanna River Transect in the Chesapeake Bay

Loading and Bioavailability of Colloidal Phosphorus in the Estuarine Gradient of the Deer Creek‐Susquehanna River Transect in the Chesapeake Bay

Perspectives from the Fritz‐Scheffer Awardee 2019. Oxygen isotopes in phosphate—The key to phosphorus tracing?

A rapid ammonium fluoride method to determine the oxygen isotope ratio of available phosphorus in tropical soils

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