The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis

Hommel, M.; Peters, W.; Ranque, J; Quilici, M; Lanotte, G.

doi:10.1080/00034983.1978.11719308

Cited by 126 publications

(59 citation statements)

References 6 publications

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“…These methods tend to be created according to the preferences of each laboratory, and may cause discordant results between different laboratories. In addition, crude antigen preparations made from whole promastigotes or their soluble extracts limit the standardization of testing and the reproducibility of results [26][27][28] . When using ELISA as a serological test, we found significantly conflicting results when different antigen preparations were used.…”

Section: Discussionmentioning

confidence: 99%

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Lima

Gennari

Soares

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Section: Discussionmentioning

confidence: 99%

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Lima

Gennari

Soares

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…The most frequently reported cross-reactions occur in areas where diseases such as Chagas' disease and African trypanosomiasis co-exist with kala-azar. 17,18 As these two diseases do not occur in Yemen, this was not a problem in our setting, but crossreactions from cutaneous leishmaniasis 19,20 can affect specificity, In spite of some reports of cross-reaction of anti-leishmania antibodies with malaria, TB, and Hansen's disease, [18][19][20][21][22] Choudhry at al. did not observe any such cross-reactions.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Kala-Azar Among Humans and Dogs in Yemen

Al-Shamahy

1998

Ann Saudi Med

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HA Al-Shamahy, Seroprevalence of Kala-Azar Among Humans and Dogs in Yemen. 1998; 18(1): 66-68 Kala-azar (visceral leishmaniasis) is a major cause of fever in the world, and is estimated to affect 100 million people worldwide.1 Kala-azar was first reported from the northern part of Yemen over 90 years ago. 2 Sporadic cases of the disease, however, are now widely reported from all over the country, including the governorates of Sana'a, Taiz, Ibb, Al-Hodeidah, Hajjah, Damar, Sa'adah, Al-Mahweet, Mareb, and Al-Jawf. Nearly 4047 cases, mostly in young children, have been reported during the last decade.3 The scarce information of the disease epidemiology in Yemen showed that the causative organisms were Leishmania donovani complex and L. infantum complex, and vectors were Phlebotomus orientalis and P. arbicus for L. infantum and L. donovani. [4][5][6] In addition, a few other infantile cases were reported from the northern part of Yemen.7,8 Michie 9 described four adult cases from the southern part of Yemen.Canine leishmaniasis was discovered by Nicole and Comte in 1908, 10 and since then reports of infected dogs have come from almost all human visceral leishmaniasis foci in the Mediterranean area.11-13 Dogs are an efficient reservoir host because parasitized fixed macrophages are so abundant in the dermal layer that parasites are readily taken up by feeding sandflies.14,15 Canine leishmaniasis was reported by Rioux et al. from human visceral leishmaniasis focus in the Taiz area of Yemen.The objectives of this study were to determine the seroprevalence of kala-azar among children in selected localities in Yemen, and to identify the natural reservoir of human kala-azar in these areas, focusing particularly on dogs, with a view to determining infection rates among them and their role in the transmission of the disease to humans. Materials and MethodsThe study was conducted in December 1993 in three selected villages where cases of kala-azar had been reported during the year, and also in the city of Sana'a. The study population consisted of volunteer schoolchildren in Beni-Mansur (Al-Haymah, Sana'a province), Huth (Sana'a province) and in Sana'a city. Based on an expected kala-azar prevalence of 10% and the worst acceptable value of 4%, not less than 270 randomly selected subjects were needed out of a total estimated 100,000 children, at a confidence level of 99.9%.A systematic random sampling of every fifth child was done. A sample of 285 subjects were selected, comprising 68 subjects from Beni-Mansur, 85 from Huth, 75 from Sharis and 57 from Sana'a city.All study participants completed an epidemiological questionnaire and gave informed consent to participate in the study. Basic demographic data, including age, sex, address, and altitude of the house, were obtained, as well as potential risks factors for kala-azar. Serological AssayA 5 mL aliquot of venous blood was allowed to clot and the serum was separated and stored at -20°C until serological analyses were performed. All the sera were tested for kala-azar using ...

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“…In order to develop specific assays for the serodiagnosis of leishmaniasis, several promastigote and amastigote antigens, purified antigens such as FML, defined, synthetic peptides or recombinant antigens have been characterized and evaluated. Among the many antigens used in ELISA for the diagnosis of leishmaniasis are total soluble antigen (Hommel et al 1978), dp72 Zalis 1988), gp 63 (Okong'o-Odera et al 1993), 78 kDa (Ravindran et al 2004) and rK39, a recombinant and a kinesin-related protein (Burns et al 1993). Some other antigens from L. infantum such as the acidic ribosomal proteins P2a and P2b, the ribosomal protein P0, the histones H2A (Soto et al 1995) and H3 were isolated and characterized for diagnosing canine VL.…”

Section: Discussionmentioning

confidence: 99%

ELISA and western blotting for the detection of Hsp70 and Hsp83 antigens of Leishmania donovani

Kaur

2012

J Parasit Dis

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The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis

Cited by 126 publications

References 6 publications

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Seroprevalence of Kala-Azar Among Humans and Dogs in Yemen

ELISA and western blotting for the detection of Hsp70 and Hsp83 antigens of Leishmania donovani

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