The Michel Benoist and Robert Mulholland yearly European Spine Journal Review: a survey of the “surgical and research” articles in the European Spine Journal, 2009

The results of this study reveal that an incidental durotomy was associated with a significant increase in the patient's length of stay, and risk for re-intervention for the treatment of persisting CSF leakage. In contrast to previous reports which have investigated the effects of incidental durotomies on the clinical outcome after lumbar decompression surgery, our data further suggest a possible inferior outcome in terms of low back pain improvement in the Dura+ cohort, which became clinically apparent at the 12-month follow-up period. Future studies should investigate whether a more pronounced decompression required for adequate exposure and repair of a dural laceration may, ultimately, result in increased segmental instability and in clinically undesirable low back pain.

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“…In case of the occurrence of a dural tear, however, varying philosophies and treatment strategies have been reported [34,35].…”

Section: Discussionmentioning

confidence: 99%

The effect of incidental dural lesions on outcome after decompression surgery for lumbar spinal stenosis: results of a multi-center study with 800 patients

et al. 2016

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“…A larger QM region than that used here (e.g., including Lys73) would be needed to model all the steps of acylation and deacylation, 17 and could be used to study alternative mechanisms, but will increase the computational demands of the calculations. If the aim is to calculate activation energies with high (e.g., approaching "chemical") accuracy, 103 which correlated ab initio methods can potentially provide, then it would also be necessary to consider zero-point energy, 46 quantum tunneling, 67,74…”

Section: Discussionmentioning

confidence: 99%

High Level QM/MM Modeling of the Formation of the Tetrahedral Intermediate in the Acylation of Wild Type and K73A Mutant TEM-1 Class A β-Lactamase

et al. 2009

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The breakdown of beta-lactam antibiotics by beta-lactamases is the most important resistance mechanism of gram negative bacteria against these drugs. The reaction mechanism of class A beta-lactamases, the most widespread family of these enzymes, consists of two main steps: acylation of an active site serine by the antibiotic, followed by deacylation and release of the cleaved compound. We have investigated the first step in acylation (the formation of the tetrahedral intermediate) for the reaction of benzylpenicillin in the TEM-1 enzyme using high level combined quantum mechanics/molecular mechanics (QM/MM) methods. Structures were optimized at the B3LYP/6-31+G(d)/CHARMM27 level, with energies for key points calculated up to the ab initio SCS-MP2/aug-cc-pVTZ/CHARMM27 level. The results support a mechanism in which Glu166 removes a proton (via an intervening water molecule) from Ser70, which in turn attacks the beta-lactam of the antibiotic. Depending on the method used, the calculated barriers range from 3 to 12 kcal mol(-1) for this step, consistent with experimental data. We have also modeled this reaction step in a model of the K73A mutant enzyme. The barrier to reaction in this mutant model is found to be slightly higher: the results indicate that Lys73 stabilizes the transition state, in particular deprotonated Ser70, lowering the barrier by about 1.7 kcal mol(-1). This finding may help to explain the conservation of Lys73, in addition to the role we have previously found for it in the later stages of the reaction (Hermann et al. Org. Biomol. Chem. 2006, 4, 206-210).

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“…Although this technique is widely used, there is a lack of consensus on either its long-term efficacy or comparative advantage over a conservative treatment (Aebi, 2009;Baerlocher et al, 2009;Bolster, 2010;Bono et al, 2010;Boszczyk, 2010;Buchbinder et al, 2009;Clark et al, 2009;Kallmes et al, 2009;Kamath and Ouellette, 2010;Kinkade and Stevermer, 2009;Mandell, 2010;Mulholland, 2010;Munk et al, 2009;Noonan, 2009;Orr, 2010;Rousing et al, 2009;Rousing et al, 2010;Weinstein, 2009).…”

Section: Introductionmentioning

confidence: 97%

Mechanism of fractures of adjacent and augmented vertebrae following simulated vertebroplasty

Wang

Chiang

Kuo

et al. 2012

Journal of Biomechanics

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The Michel Benoist and Robert Mulholland yearly European Spine Journal Review: a survey of the “surgical and research” articles in the European Spine Journal, 2009

Cited by 3 publications

References 56 publications

The effect of incidental dural lesions on outcome after decompression surgery for lumbar spinal stenosis: results of a multi-center study with 800 patients

The effect of incidental dural lesions on outcome after decompression surgery for lumbar spinal stenosis: results of a multi-center study with 800 patients

High Level QM/MM Modeling of the Formation of the Tetrahedral Intermediate in the Acylation of Wild Type and K73A Mutant TEM-1 Class A β-Lactamase

Mechanism of fractures of adjacent and augmented vertebrae following simulated vertebroplasty

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