The methylotrophic yeastHansenula polymorpha: its use in fundamental research and as a cell factory

Gellissen, Gerd; Veenhuis, Marten

doi:10.1002/1097-0061(200102)18:3<::aid-yea695>3.0.co;2-9

Cited by 18 publications

(12 citation statements)

References 13 publications

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“…The methylotrophic yeast H. polymorpha ( previously Pichia angusta or Ogataea polymorpha) was first studied as model organism for peroxisome function as well as nitrate assimilation [14] , [15] , [76] , [99] . The availability of a strong inducible expression system coupled with effective protein secretion and glycosylation has also made H. polymorpha a successful host for protein production.…”

Section: Bioprocessing and Metabolic Engineering With Non-conventionamentioning

confidence: 99%

Genome and metabolic engineering in non-conventional yeasts: Current advances and applications

Löbs

Schwartz

Wheeldon

2017

Synthetic and Systems Biotechnology

116

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Microbial production of chemicals and proteins from biomass-derived and waste sugar streams is a rapidly growing area of research and development. While the model yeast Saccharomyces cerevisiae is an excellent host for the conversion of glucose to ethanol, production of other chemicals from alternative substrates often requires extensive strain engineering. To avoid complex and intensive engineering of S. cerevisiae, other yeasts are often selected as hosts for bioprocessing based on their natural capacity to produce a desired product: for example, the efficient production and secretion of proteins, lipids, and primary metabolites that have value as commodity chemicals. Even when using yeasts with beneficial native phenotypes, metabolic engineering to increase yield, titer, and production rate is essential. The non-conventional yeasts Kluyveromyces lactis, K. marxianus, Scheffersomyces stipitis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris have been developed as eukaryotic hosts because of their desirable phenotypes, including thermotolerance, assimilation of diverse carbon sources, and high protein secretion. However, advanced metabolic engineering in these yeasts has been limited. This review outlines the challenges of using non-conventional yeasts for strain and pathway engineering, and discusses the developed solutions to these problems and the resulting applications in industrial biotechnology.

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Section: Bioprocessing and Metabolic Engineering With Non-conventionamentioning

confidence: 99%

Genome and metabolic engineering in non-conventional yeasts: Current advances and applications

Löbs

Schwartz

Wheeldon

2017

Synthetic and Systems Biotechnology

116

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“…During the last decade methylotrophic yeasts have gained increasing interest, both for fundamental (van der Klei and Veenhuis, 1996; Subramani, 1998) and applied purposes (Cereghino and Cregg, 2000; Gellisen, 2000). Currently, the H. polymorpha system provides a remarkably versatile technology platform for heterologous gene expression (Gellisen and Veenhuis, 2001). The Hansenula toolbox includes a variety of host strains, various sites available for integration and several strong promoters (reviewed by van Dijk et al ., 2000).…”

Section: Introductionmentioning

confidence: 99%

Transfer Hydrogenation Processes toμ3-Alkylidyne Groups on the Organotitanium Oxide [Ti3CpO3]

et al. 2002

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“…The methylotrophic yeast H. polymorpha has been successfully developed as a host for the production of foreign proteins . High-level protein production is facilitated by the availability of strong, inducible promoters (P AOX , P FMD ) (van Dijk et al, 2000;Gellissen & Veenhuis, 2001). We aimed to utilize the favorable properties of H. polymorpha, via metabolic reprogramming, for synthesis of b-lactam antibiotics.…”

Section: Discussionmentioning

confidence: 99%

ReprogrammingHansenula polymorphafor penicillin production: expression of thePenicillium chrysogenum pclgene

Gidijala

Klei

Veenhuis

et al. 2007

FEMS Yeast Research

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We aim to introduce the penicillin biosynthetic pathway into the methylotrophic yeast Hansenula polymorpha. To allow simultaneous expression of the multiple genes of the penicillin biosynthetic pathway, additional markers were required. To this end, we constructed a novel host-vector system based on methionine auxotrophy and the H. polymorpha MET6 gene, which encodes a putative cystathionine beta-lyase. With this new host-vector system, the Penicillium chrysogenum pcl gene, encoding peroxisomal phenylacetyl-CoA ligase (PCL), was expressed in H. polymorpha. PCL has a potential C-terminal peroxisomal targeting signal type 1 (PTS1). Our data demonstrate that a green fluorescent protein-PCL fusion protein has a dual location in the heterologous host in the cytosol and in peroxisomes. Mutation of the PTS1 of PCL (SKI-COOH) to SKL-COOH restored sorting of the fusion protein to peroxisomes only. Additionally, we demonstrate that peroxisomal PCL-SKL produced in H. polymorpha displays normal enzymatic activities.

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The methylotrophic yeastHansenula polymorpha: its use in fundamental research and as a cell factory

Cited by 18 publications

References 13 publications

Genome and metabolic engineering in non-conventional yeasts: Current advances and applications

Genome and metabolic engineering in non-conventional yeasts: Current advances and applications

Transfer Hydrogenation Processes toμ3-Alkylidyne Groups on the Organotitanium Oxide [Ti3CpO3]

ReprogrammingHansenula polymorphafor penicillin production: expression of thePenicillium chrysogenum pclgene

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