The Methodology Applied in DPPH, ABTS and Folin-Ciocalteau Assays Has a Large Influence on the Determined Antioxidant Potential

Abramovič, Helena; Grobin, Blaž; Ulrih, Nataša Poklar; Cigić, Blaž

doi:10.17344/acsi.2017.3408

Cited by 38 publications

(25 citation statements)

References 38 publications

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“…Caffeic acid and its ester (CGA) have similar yet kinetically different profiles. On the contrary to GA, contribution of secondary reactions in DPPH assay at pH 7.4 is We have observed the sample speci c kinetic pro les also for di erent food matrices [22]. e dΔA/dt on the time scale of few tens of minutes when samples as tea, co ee, cranberry, and apple juice were analyzed was even larger than observed for antioxidant compounds in this study.…”

Section: E Influence Of Solvent On the Kinetics Of Reaction Ofcontrasting

confidence: 53%

“…AOP of food samples is usually evaluated by more than one method, and correlation analysis of AOPs obtained by DPPH, ABTS, and FC assays is often performed. e correlations can be high and significant or weak [20][21][22], reflecting the lack of consistency of the results of such analyses. e large influence of the experimental parameters on the reactivity of antioxidants in the samples undoubtedly contributes to the observed discrepancies.…”

Section: Introductionmentioning

confidence: 99%

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Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu

Abramovič

Grobin

Ulrih

et al. 2018

Journal of Chemistry

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Trolox, gallic acid, chlorogenic acid, caffeic acid, catechin, epigallocatechin gallate, and ascorbic acid are antioxidants used as standards for reaction with chromogenic radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH⋅) and 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS⋅+), and Folin–Ciocalteu (FC) reagent. The number of exchanged electrons has been analyzed as function of method and solvent. A majority of compounds exchange more electrons in FC assay than in ABTS and DPPH assays. In reaction with chromogenic radicals, the largest number of electrons was exchanged in buffer (pH 7.4) and the lowest reactivity was in methanol (DPPH) and water (ABTS). At physiological pH, the number of exchanged electrons of polyphenols exceeded the number of OH groups, pointing to the important contribution of partially oxidized antioxidants, formed in the course of reaction, to the antioxidant potential. For Trolox, small impact on the number of exchanged electrons was observed, confirming that it is more suitable as a standard compound than the other antioxidants.

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Section: E Influence Of Solvent On the Kinetics Of Reaction Ofcontrasting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu

Abramovič

Grobin

Ulrih

et al. 2018

Journal of Chemistry

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“…Therefore, it is impossible to determine exactly the type and the amount of the contained antioxidants. The use of various tests can instead lead to results that are able to differentiate the whole matrice in their heterogeneity and complexity and better describe their nutritional values (Shahidi & Ambigaipalan, 2015;Shahidi & Zhong, 2015;Abramovi c et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Geographical characterisation of multifloral honeys from the Marche region (Italy) according to their antioxidant activity and colour using a chemometric approach

Castiglioni

Stefano²,

Pisani

et al. 2017

Int J of Food Sci Tech

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Multifloral honeys produced in the Marche region (Italy) were analysed leading to the antioxidant and colour characterisation of this type of regional honeys: the antioxidant activity was determined measuring total phenolic content (TPC), and using ABTS and DPPH assays. The elaboration of data with multivariate analyses enabled differentiation of the geographical origin of honey samples, namely as coming from the Mid/Coastal Hilly or from the High Hilly/Submountain belt of the Marche region. The cluster analysis applied to a selection of samples divided honeys belonging to the Mid/Coastal Hilly belt into three groups showing low, medium and high antioxidant activity and in part located in different areas of the region. Finally, using data of unifloral honeys from the Marche region previously determined, it was possible to have an indication of the prevalent nectar contained in most of the multifloral honey samples harvested in 2012 in the Marche region.

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“…The first step to quantify the antioxidant activity of a plant extract is to select the right method [9]. There is a large variety of methods to determine this parameter [10], and the variability of experimental conditions found in the literature for each of the methods hinders such selection and the possibility of easily comparing the obtained results with those of other authors.…”

Section: Introductionmentioning

confidence: 99%

Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used

2020

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Plants have a large number of bioactive compounds with high antioxidant activity. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to determine which species have the highest antioxidant activity. The aim of this work was to verify whether different methods show the same sensitivity and/or capacity to discriminate the antioxidant activity of the extract of different plant species. To that end, we selected 12 species with different content of phenolic compounds. Their extracts were analyzed using the following methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, ferric reducing (FRAP) assay, Trolox equivalent antioxidant capacity (ABTS) assay, and reducing power (RP) assay. The four methods selected could quantify the antioxidant capacity of the 12 study species, although there were differences between them. The antioxidant activity values quantified through DPPH and RP were higher than the ones obtained by ABTS and FRAP, and these values varied among species. Thus, the hierarchization or categorization of these species was different depending on the method used. Another difference established between these methods was the sensitivity obtained with each of them. A cluster revealed that RP established the largest number of groups at the shortest distance from the root. Therefore, as it showed the best discrimination of differences and/or similarities between species, RP is considered in this study as the one with the highest sensitivity among the four studied methods. On the other hand, ABTS showed the lowest sensitivity. These results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter.

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The Methodology Applied in DPPH, ABTS and Folin-Ciocalteau Assays Has a Large Influence on the Determined Antioxidant Potential

Cited by 38 publications

References 38 publications

Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu

Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu

Geographical characterisation of multifloral honeys from the Marche region (Italy) according to their antioxidant activity and colour using a chemometric approach

Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used

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