The Methanol--Sulfuric Acid Esterification Methods. II. An Improved Extraction Procedure

Rogozinski, M.

doi:10.1093/chromsci/2.10.328

Cited by 45 publications

(9 citation statements)

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“…A known amount of decanoic acid in methanol was added as internal standard, and the residue was made up with methanol to a final volume of 2 ml. The fatty acids were con-verted to their methyl esters and extracted with hexane as described by Rogozinski [37]. 5 p1 of the hexdne extract was injected into a Hewlett-Packard 5750 G gas chromatograph equipped with a flame ionisation detector.…”

Section: Analyses Of the Perfusion Mediummentioning

confidence: 99%

Effects of Octanoate and Oleate on Energy Metabolism in the Perfused Rat Liver

Debeer

Mannaerts

Schepper

1974

European Journal of Biochemistry

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The effects of octanoate and oleate were studied in the isolated fasted rat liver perfused without substrate and were compared with the effects of lactate. Measurements were made of hepatic adenine nucleotide content, formation of ketone bodies, urea and glucose and uptake of fatty acids, lactate and oxygen. Calculations of the Krebs cycle activity and the flux through the oxidation at C-3 were carried out. Comparing the extra oxygen consumption with extra ATP needs after addition of the fatty acids or lactate, ADP : 0 ratios were estimated for fatty acid oxidation and lactate gluconeogenesis.1. Octanoate as well as oleate induced a net decrease in 4 T P and an increase in AMP content of the liver. Total adenine nucleotides were unaltered. ATP : ADP ratios were lowered while the adenylate kinase mass-action ratio increased.2. The Krebs cycle activity was suppressed by both fatty acids and enhanced by lactate. Oxidation at C-3 was strongly stimulated by the fatty acids and was unaffected by lactate.3. An apparent ADP : 0 ratio of 2.7 was obtained after lactate addition, indicating a tightly coupled oxidative phosphorylation for the liver preparations used. Octanoate and oleate gave extremely low ratios of near one and near zero respectively.4. Perfusion with oligomycin caused a severe drop in oxygen uptake by the liver, which was unaltered after addition of fatty acids. Oligomycin added after the fatty acids caused an immediate fall in oxygen uptake to the level observed with oligomycin alone. 2,CDinitrophenol was able to stimulate the oligomycin-depressed respiration, both in the absence and in the presence of fatty acids. These results indicate that under our experimental conditions the fatty acids had no uncoupling effect and that microsomal fatty acid oxidation had to be minimal.5. Incorporation of [3H]leucine into proteins in liver and medium remained unchanged after addition of fatty acids, indicating that the low apparent ADP : 0 ratios are not the result of an enhanced synthesis of proteins.6. The possible relationship between fatty acid transport, energy-wasting futile cycles and the low apparent ADP : 0 ratios observed during fatty acid oxidation is discussed.Enzynies. 3-Hydroxy-3-methylglutaryl-CoA reductase or mevalonate : NADPf oxidoreductase (CoA-acylating) (EC

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Section: Analyses Of the Perfusion Mediummentioning

confidence: 99%

Effects of Octanoate and Oleate on Energy Metabolism in the Perfused Rat Liver

Debeer

Mannaerts

Schepper

1974

European Journal of Biochemistry

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“…Subsequent methylation of the free fatty acids was accomplished according to the method of Rogozenski (1964).…”

Section: Fatty Acid Compositionmentioning

confidence: 99%

“…The lipid extracts of whole yolk were saponified and free fatty acids were prepared according to Ast (1963). Subsequent methylation of the free fatty acids was accomplished according to the method of Rogozenski (1964).…”

Section: Fatty Acid Compositionmentioning

confidence: 99%

Effect of Dietary Fats on Some Chemical and Functional Properties of Eggs

Pankey¹,

Stadelman²

1969

Journal of Food Science

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SUMMARY —Laying pullets were fed a low fat semipurified diet or the low fat diet supplement with 10% vegetable oil (corn, soybean, olive, safflower or hydrogenated coconut oil). The eggs were analyzed for change in the fatty acid composition of the total yolk lipids with time on the diet and for fatty acid composition of the triglyceride, cephalin and lecithin fractions of lipovitellin and lipovitellenin. Determinations were made for volume of sponge cakes, emulsification, and lipid content of stored eggs. A taste panel was used to assess any difference in flavor and mouth feel of yolk from stored eggs. The fatty acid composition of the total yolk lipids was influenced by all dietary fats. The major change was in the linoleic acid at the expense of oleic acid with corn, soybean and safflower oil. Olive oil increased the oleic acid and hydogenated coconut oil increased lauric, myristic and myristoleic acids. The fatty acid composition of the fractions of the lipoproteins was influenced by the dietary fats and varied between fractions. Differences were noted between sponge cake volume with eggs of low fat, corn oil, soybean oil and hydrogenated coconut oil diets. The dietary fats did not appear to affect emulsification capacity or migration of the yolk. A taste panel was unable to differentiate on the basis of flavor or mouth feel the egg yolk from the several treatments.

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“…The resulting plasma was extracted ac cording to Bligh and Dyer (1), and neutral lipids separated from phospholipids by column chromatog raphy on silicic acid according to Wood and Snyder (27). Portions of the neutral lipid and phospholipid extracts were dried under N2 in screw cap test tubes and the methyl esters of their fatty acids prepared according to Rogozinski (18). Placental homogenates or microsomal fractions (prepared by differential centrifugation) were extracted according to Bligh and Dyer (1) and portions of the chloroform extracts chromatographed, along with authentic standards, on Adsorbasil-5 (Applied Science Laboratories, State College, Pa.) in CHC13:H3C0H:H20 (65:25:4, v/v) or silica gel 60 HR (EM Laboratories, Elmsford, N.Y.) in CHC13:H3C0H:acetic acid:H20 (50:25:6:1, v/v).…”

Section: Methodsmentioning

confidence: 99%

“…The phospholipids were visualized under UV light after spraying the chromatographs lightly with either 2,7-dichlorofluorescein (0.05% in 95% ethanol, w/v) or Rhodamine B (0.025% in 95% ethanol, w/v). The silica gel containing phosphatidylcholine or 1,2-diacyl-snglycero-3-phosphoethanolamine (diacyl-GPE) was scraped into screw cap test tubes followed by acid methanolysis according to Rogozinski (18). Methyl esters were separated on either a glass 6-ft. U tube, packed with 10% Silar 10C on gas chrom Q (Applied Science Laboratories), in a Barber-Coleman 5000 series oven equipped with an H2 flame detector and programmed to have an initial temperature of 165 °C, rising 3°/min to a final temperature of 235 °C; or on a 6-ft., coiled, stainless steel tube packed with 10% EGSS-X on gas chrom P (Applied Science Labora tories) at 195 °C in a Perkin-Elmer 3920 oven equipped with an H2 flame detector.…”

Section: Methodsmentioning

confidence: 99%

Deacylation-Reacylation of Phospholipids by Rat Embryos and Their Associated Placentas

East¹,

Chepenik²,

Waite³

1979

Neonatology

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Homogenates and subcellular fractions of rat chorioallantoic placentas obtained on the 14th day of gestation were assayed for acyl-CoA: lysophospholipid acyltransferase activities using (¹⁴C)-linoleic acid, ATP, and CoA, or (¹⁴C)-oleoyl-CoA as acyl donor and 1-acylglycerophosphorylcholine or 1-acylglycerophosphorylethanolamine as acyl acceptor. We found that: (1) the Lands’ deacylation-reacylation cycle is present in the rat placenta, (2) the enzymes were concentrated in the microsomal fraction, and (3) the optimal conditions for in vitro assay of those enzyme activities were similar to those reported for adult tissues. Comparison of the acyl composition of phospholipids in maternal blood with those in placental homogenates revealed similarities which are consistent with a role for this cycle in vivo in placental uptake of phospholipids. That this cycle may function also in placental transformation of phospholipids, in vivo, is suggested by differences between the acyl composition of phospholipids in placental homogenates and microsomal fractions.

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The Methanol--Sulfuric Acid Esterification Methods. II. An Improved Extraction Procedure

Cited by 45 publications

References 0 publications

Effects of Octanoate and Oleate on Energy Metabolism in the Perfused Rat Liver

Effects of Octanoate and Oleate on Energy Metabolism in the Perfused Rat Liver

Effect of Dietary Fats on Some Chemical and Functional Properties of Eggs

Deacylation-Reacylation of Phospholipids by Rat Embryos and Their Associated Placentas

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