The methanol-soluble fraction of <i>Millettia macrophylla</i> (Fabaceae) stem bark endowed with estrogenic properties has adverse effects on the male reproductive system of Wistar rats

Zingue, Stéphane; Nde, Chantal Beatrice Magne; Njamen, Dieudonné

doi:10.1515/jbcpp-2013-0136

Cited by 5 publications

(2 citation statements)

References 46 publications

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“…M. macrophylla has demonstrated pharmacological effects on estrogen target organs of female [ 18 ], osteoporosis [ 21 ], and male reproductive system of Wistar rats [ 22 ]. We hypothesize that M. macrophylla might mediate some of its actions by binding to the estrogen receptor, as it shares many estrogen-like actions in various physiological systems.…”

Section: Discussionmentioning

confidence: 99%

Elucidation of Underlying Mechanisms by WhichMillettia macrophyllaBenth Induces Its Estrogenic Activity

Zingue

Nde

Clyne

et al. 2014

International Scholarly Research Notices

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Millettia macrophylla is used traditionally to treat menopause related symptoms. This plant was shown to exhibit estrogenic effects in vitro on human embryonic kidney cells and in vivo on ovariectomized rats. The present study aimed at elucidating underlying mechanisms by which M. macrophylla induced its estrogenic effects. To accomplish our goal, kidney Hek293T cells transiently transfected with estrogen alpha or beta receptor expression plasmids were cotreated with a pure antiestrogen ICI 182,780 and the dichloromethane or methanol soluble fractions of M. macrophylla. To follow up, we cotreated ovariectomized rats with both extracts and ICI 182,780 for 3 days in the classical uterotrophic assay. Animals were then sacrificed and the uterine wet weight, total protein levels in uteri, uterine, and vaginal epithelial heights, and mammary gland were assessed. In vitro, the results suggested that the induction of the estrogenic activity by M. macrophylla is due to the binding of its secondary metabolites to ERα and ERβ. In vivo, the cotreatment of extracts and ICI 182,780 significantly abrogated the biological responses induced by the extracts alone. Taken together, these results indicate that the active principles of M. macrophylla induce their beneficial effects on menopausal symptoms by activating the ERs.

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Section: Discussionmentioning

confidence: 99%

Elucidation of Underlying Mechanisms by WhichMillettia macrophyllaBenth Induces Its Estrogenic Activity

Zingue

Nde

Clyne

et al. 2014

International Scholarly Research Notices

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“…Treatments were given by gavage for 8 consecutive days. Male sexual behavior parameters such as copulation and male-oriented activity (latencies of first mount, intromission and ejaculation, numbers of mount, intromission, ejaculation and penile licking) towards an estrus female were recorded for 30 min by blinded observers on days 1 and 8 in a quiet room under dim light [52] . Animals were sacrificed by decapitation on day 9 and organs including right testis, epididymis, seminal vesicles, ventral prostate and penis were collected and weighed.…”

Section: Aphrodisiac Activity In Normal Ratsmentioning

confidence: 99%

Baillonella toxisperma improves sexual performance and protects against stress-induced reproductive dysfunction in male Wistar rats

Simo¹,

Ateba²,

Zingue³

et al. 2019

J Phytopharmacol

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Baillonella toxisperma Pierre (Sapotaceae) is a medicinal plant widely used in Central Africa against several diseases including erectile dysfunction and male infertility. However, no study dealing with these male reproductive dysfunctions has been published until now. Accordingly, the present study was undertaken. It evaluated the capacity of an aqueous extract of stem bark of B. toxisperma (BT) at 75, 150 and 300 mg/kg/d to induce aphrodisiac effects and prevent the stress-induced reproductive dysfunction in males using normal non-experienced (an 8-day oral treatment) and stressed (a 6-h/day immobilization stress for 35 consecutive days)male Wistar rats. Both in normal and stressed animals, BT at the doses of 150 and 300 mg/kg decreased (p < 0.05) the mount and intromission latencies, and increased (p < 0.05) the number of total penile licking, mount and intromission as well as ejaculation frequencies. In stressed animals, a significant (p < 0.05) increase in sperm levels, sperm mobility as well as in the relative weight of androgen-dependent organs (testis, epididymis and seminal vesicles) was observed at the same doses compared with the stressed control. These results suggest that this aqueous extract of B. toxisperma might endow with aphrodisiac and androgenic properties in normal and stressed male Wistar rats.

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Soybean isoflavone affects in rabbits: Effects on metabolism, antioxidant capacity, hormonal balance and reproductive performance

Abo-Elsoud

Hashem

El-Din

et al. 2019

Animal Reproduction Science

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The methanol-soluble fraction of Millettia macrophylla (Fabaceae) stem bark endowed with estrogenic properties has adverse effects on the male reproductive system of Wistar rats

Abstract: These results suggest that long-term exposure to the estrogenic MeOH fraction of M. macrophylla stem bark negatively alters sexual behavior and spermatogenesis.

Cited by 5 publications

References 46 publications

Elucidation of Underlying Mechanisms by WhichMillettia macrophyllaBenth Induces Its Estrogenic Activity

Elucidation of Underlying Mechanisms by WhichMillettia macrophyllaBenth Induces Its Estrogenic Activity

Baillonella toxisperma improves sexual performance and protects against stress-induced reproductive dysfunction in male Wistar rats

Soybean isoflavone affects in rabbits: Effects on metabolism, antioxidant capacity, hormonal balance and reproductive performance

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