The Metachromatic Staining Reaction

Abstract

THE METACHROMATIC STAINING REACTION Lison's (1935) classical studies on the metachromatic staining reaction led him to conclude that the capacity to stain metachromatically was the exclusive property of sulphuric acid esters of high molecular weight. He stipulated that the reaction was specific only if carried out under conditions unfavourable to the development of the metachromatic colour, and suggested that this is most easily AND G. M. WINDRUM

Biosynthesis of intestinal mucins. 4. Utilization of [1-14C]glucose by sheep colonic mucosa in vitro

“…Hydrolysed components were separated in the ethanol-ammonium acetate solvent of , after removal of the acid in vacuo. Acidic mucosubstances were metachromatically stained with Azur A (0-1 % in 70 % ethanol; Kramer & Windrum, 1955). Oxidizable products were detected by the periodate-Schiff reaction (Bj6rnesj6, 1955).…”

A Neoplastic Connective Tissue Mast Cell Capable of Continuous Growth In Tissue Culture

“…Fixatives appropriate for the staining procedures were used. The metachromasy of fixed cells was demonstrated by staining with 0.1 per cent azure A (National NAz 12) in 30 per cent ethanol (26). Dehydration and clearing of these preparations were accomplished by immersing the coverslips sequen tially in acetone, acetone-xylene 2:1, acetone-xylene 1:2, and xylene.…”

Image analysis assisted study of mitotic figures in oral epithelial dysplasia and squamous cell carcinoma using differential stains

“…36 The overall higher diagnostic efficacy of Crystal Violet could also be linked to the modification of staining methodology by incorporating the use of 1 N HCl at 60 8C thereby causing increase in contrast due to reduced staining of cytoplasm, which is likely to be the consequence of reduced RNA content following hydrolysis. 13,37,38 This delineated the normal and abnormal types of mitotic figures distinctly in the superficial epithelium as well as in tumor islands.…”