The metabolome of human placental tissue: investigation of first trimester tissue and changes related to preeclampsia in late pregnancy

Dunn, Warwick B.; Brown, Marie; Worton, Stephanie; Davies, Kyle; Jones, Rebecca L.; Kell, Douglas B.; Heazell, Alexander E P

doi:10.1007/s11306-011-0348-6

Cited by 55 publications

(64 citation statements)

References 64 publications

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“…Blood plasma was the most commonly used biologic media (n = 10) (Bahado-Singh, Akolekar, Mandal, Dong, Xia, Kruger, Wishart et al 2012; De Oliveira, Câmara, Bonetti, Turco, Bertolla, Moron, Sass et al 2012; Kelly, Croteau-Chonka, Dahlin, Mirzakhani, Wu, Wan, McGeachie et al 2016; Kenny, Broadhurst, Dunn, Brown, North, McCowan, Roberts et al 2010; Kenny, Dunn, Ellis, Myers, Baker, Kell 2005; Odibo, Goetzinger, Odibo, Cahill, Macones, Nelson, Dietzen 2011; Pinto, Almeida, Martins, Duarte, Barros, Galhano, Pita et al 2015; Schott, Hahn, Kurbacher, Moka 2012; Turner, Brewster, Simpson, Walker, Fisher 2007; Turner, Brewster, Simpson, Walker, Fisher 2008), followed by serum (n = 7) (Bahado-Singh, Akolekar, Mandal, Dong, Xia, Kruger, Wishart et al 2013; Bahado-Singh, Syngelaki, Akolekar, Mandal, Bjondahl, Han, Dong et al 2015; Chen, He, Tan, Xu 2017; Kenny, Broadhurst, Brown, Dunn, Redman, Kell, Baker 2008; Koster, Vreeken, Harms, Dane, Kuc, Schielen, Hankemeier et al 2015; Kuc, Koster, Pennings, Hankemeier, Berger, Harms, Dane et al 2014), and placental or intrauterine tissue samples (n = 7) (Austdal, Thomsen, Tangerås, Skei, Mathew, Bjørge, Austgulen et al 2015; Baig, Lim, Fernandis, Wenk, Kale, Su, Biswas et al 2013; Dunn, Brown, Worton, Davies, Jones, Kell, Heazell 2012; Jain, Jayasimhulu, Clark 2004; Korkes, Sass, Moron, Câmara, Bonetti, Cerdeira, Da Silva et al 2014; Pearson, Zhang, Arya, Warren, Ortori, Fakis, Khan et al 2010; Sohlberg, Wikström, Olovsson, Lindgren, Axelsson, Mulic-Lutvica, Weis et al). The remaining studies used urine (n = 1) (Diaz, Barros, Goodfellow, Duarte, Galhano, Pita, Almeida et al 2013), urine and serum (n = 2) (Austdal, Skråstad, Gundersen, Austgulen, Iversen, Bathen 2014; Austdal, Tangerås, Skråstad, Salvesen, Austgulen, Iversen, Bathen 2015), or breast milk (n=1) (Dangat, Upadhyay, Kilari, Sharma, Kemse, Mehendale, Lalwani et al 2016).…”

Section: Resultsmentioning

confidence: 99%

Applications of metabolomics in the study and management of preeclampsia: a review of the literature

et al. 2017

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Introduction: Preeclampsia represents a major public health burden worldwide, but predictive and diagnostic biomarkers are lacking. Metabolomics is emerging as a valuable approach to generating novel biomarkers whilst increasing the mechanistic understanding of this complex condition. Objectives: To summarize the published literature on the use of metabolomics as a tool to study preeclampsia. Methods: PubMed and Web of Science were searched for articles that performed metabolomic profiling of human biosamples using either Mass-spectrometry or Nuclear Magnetic Resonance based approaches and which included preeclampsia as a primary endpoint. Results: Twenty-eight studies investigating the metabolome of preeclampsia in a variety of biospecimens were identified. Individual metabolite and metabolite profiles were reported to have discriminatory ability to distinguish preeclamptic from normal pregnancies, both prior to and post diagnosis. Lipids and carnitines were among the most commonly reported metabolites. Further work and validation studies are required to demonstrate the utility of such metabolites as preeclampsia biomarkers. Conclusion: Metabolomic-based biomarkers of preeclampsia have yet to be integrated into routine clinical practice. However, metabolomic profiling is becoming increasingly popular in the study of preeclampsia and is likely to be a valuable tool to better understand the pathophysiology of this disorder and to better classify its subtypes, particularly when integrated with other omic data.

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Section: Resultsmentioning

confidence: 99%

Applications of metabolomics in the study and management of preeclampsia: a review of the literature

et al. 2017

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“…However, the study included only relatively polar metabolites, so balanced extraction of both polar and non-polar metabolites may not be achievable with the proposed conditions. Methods that can simultaneously extract both hydrophilic and lipophilic species, for example chloroform-methanol or chloroformmethanol-water extraction are also popular, but use of chloroform is problematic in LC-MS and necessitates further sample manipulation, for example lyophilization, before injection [11,35,85]. Sun et al proposed a methanol-chloroform-water extraction procedure for analysis of mouse heart tissue to extract both lipophilic and hydrophilic species and eliminate ionization suppression effects observed with perchloric acid extraction [37].…”

Section: Tissue Metabolomicsmentioning

confidence: 99%

Current trends and challenges in sample preparation for global metabolomics using liquid chromatography–mass spectrometry

2012

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The choice of sample-preparation method is extremely important in metabolomic studies because it affects both the observed metabolite content and biological interpretation of the data. An ideal sample-preparation method for global metabolomics should (i) be as non-selective as possible to ensure adequate depth of metabolite coverage; (ii) be simple and fast to prevent metabolite loss and/or degradation during the preparation procedure and enable high-throughput; (iii) be reproducible; and (iv) incorporate a metabolism-quenching step to represent true metabolome composition at the time of sampling. Despite its importance, sample preparation is often an overlooked aspect of metabolomics, so the focus of this review is to explore the role, challenges, and trends in sample preparation specifically within the context of global metabolomics by liquid chromatography-mass spectrometry (LC-MS). This review will cover the most common methods including solvent precipitation and extraction, solid-phase extraction and ultrafiltration, and discuss how to improve analytical quality and metabolite coverage in metabolomic studies of biofluids, tissues, and mammalian cells. Recent developments in this field will also be critically examined, including in vivo methods, turbulent-flow chromatography, and dried blood spot sampling.

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“…Hence, it can clearly lter out the variation in metabolite signals between sample groups. 44) Furthermore, XCMS Online has been used on a wide variety of applications, including researches of cancer, [54][55][56][57][58] pregnancy syndromes, 59) and variability analysis of body uids. 60) e work ow of XCMS Online was shown in Fig.…”

Section: Methods Of Mass Spectrometry In Dis-covery Of Exposure Markermentioning

confidence: 99%

Exposure Marker Discovery of Phthalates Using Mass Spectrometry

2017

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Phthalates are chemicals widely used in industry and the consequences on human health caused by exposure to these agents are of signi cant interest currently. e urinary metabolites of phthalates can be measured and used as exposure markers for the assessment of the actual internal contamination of phthalates coming from di erent sources and absorbed by various ways. e purpose of this paper is to review the markers for exposure and risk assessment of phthalates such as di-methyl phthalate (DMP), di-ethyl phthalate (DEP), di-butyl phthalate (DBP), benzylbutyl phthalate (BBP), di-(2-ethylhexyl)phthalate (DEHP), di-(2-propylheptyl)phthalate (DPHP), di-iso-nonyl phthalate (DINP), di-n-octyl phthalate (DnOP) and di-isodecyl phthalate (DIDP), and introduction of the analytical approach of three metabolomics data processing approaches that can be used for chemical exposure marker discovery in urine with high-resolution mass spectrometry (HRMS) data.

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The metabolome of human placental tissue: investigation of first trimester tissue and changes related to preeclampsia in late pregnancy

Cited by 55 publications

References 64 publications

Applications of metabolomics in the study and management of preeclampsia: a review of the literature

Applications of metabolomics in the study and management of preeclampsia: a review of the literature

Current trends and challenges in sample preparation for global metabolomics using liquid chromatography–mass spectrometry

Exposure Marker Discovery of Phthalates Using Mass Spectrometry

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