The Metabolites of the Herbicide L-Phosphinothricin (Glufosinate) (Identification, Stability, and Mobility in Transgenic, Herbicide-Resistant, and Untransformed Plants)

Dröge‐Laser, Wolfgang; Siemeling, Ulrich; Pühler, Alfred; Broer, Inge

doi:10.1104/pp.105.1.159

Cited by 75 publications

(49 citation statements)

References 17 publications

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“…A rapid decarboxylation reaction then results in the stable main metabolite 3-methylphosphinico-propionic acid (MPP) which is non-phytotoxic. Within non-transgenic plants, PPO can also be reduced to form 4-methyl-phosphinico-2-hydroxy-butanoic acid, another final and stable product (Droege-Laser et al, 1994). In contrast to transgenic PAT-expressing plants, there is no direct proof that in non-tolerant plants only the L-isomer is metabolized.…”

Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

“…In corn forage, in soybean seed, in sugar beet roots and in tomato fruit, the main residue was N-acetyl-L-glufosinate. Experiments of Droege et al (1992) and Droege-Laser et al (1994) using transgenic tobacco, carrot, and alfalfa plants also found N-acetyl-Lglufosinate as the major metabolite in glufosinate-tolerant plants. Besides the principal residues, trace levels of other metabolites were also identified in soybean including 2-methylphosphinico-acetic acid (MPA) and 4-methylphosphinico-butanoic acid (MPB).…”

Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

“…There is limited translocation of glufosinate within the plant. After application of L-glufosinate, N-acetyl-L-glufosinate and further metabolites on distinct leaves, a preferential transport into the upper leaves and a low level of translocation into the lower plant parts was observed in both genetically modified and unmodified tobacco plants (Droege, 1991;Droege-Laser et al, 1994).…”

Section: Section I -Biochemistry and Physiology Of The Herbicide In Nmentioning

confidence: 99%

“…In genetically modified glufosinate-tolerant plants expressing the PAT enzyme, it appears that two metabolic routes compete: (1) the deamination of glufosinate and subsequent conversion of 4-methylphosphinico-2-oxo-butanoic acid (PPO) to 3-methylphosphinico-propionic acid (MPP) or to 4-methylphosphinico-2-hydroxy-butanoic acid, and (2) the N-acetylation of L-glufosinate by PAT (Droege-Laser et al, 1994). The second of these two routes predominates when PAT specific activity is relatively high.…”

Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

“…The metabolism of glufosinate in artificial systems like cell suspension cultures (soybean, wheat, maize) and sterile plants (tobacco, alfalfa, carrot) has been analyzed by Komossa and Sandermann (1992) and by Droege-Laser et al (1994). After treatment of non-transgenic plants with glufosinate, the unstable intermediate 4-methylphosphinico-2-oxo-butanoic acid (PPO) is formed via deamination.…”

Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

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Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Barnett

Culpepper²,

York

et al. 2015

Weed technol.

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Glyphosate-resistant (GR) weeds are most challenging for growers to control in cotton in the midsouth region of the United States. As a result, growers in these states are transitioning from a glyphosate-based system to a glufosinate-based system. A field study was conducted in 2010 and 2011 in Georgia, North Carolina, and Tennessee to determine the effect of repeated glufosinate applications to WideStrike cotton applied at various application timings. One to three glufosinate applications at 594 g ai ha−1were applied to cotton at the two-leaf, seven-leaf, bloom, or 2 wk after bloom stages. Visual estimates of crop injury were evaluated after each application, in addition to crop height, development, and yield. Crop injury after each glufosinate application typically ranged from 3 to 11%, with higher injury observed where multiple glufosinate applications were made. Crop height differences were noted after the bloom and 2 wk after bloom applications. Treatments that had one, two, or three glufosinate applications had reduced crop height when compared with the weed-free control. Node above cracked boll ratings also indicated that glufosinate treatments may have stressed the cotton. Ultimately, yield was reduced where three glufosinate treatments were applied to WideStrike cotton. In addition, one glufosinate application at the bloom stage or two glufosinate applications at the bloom and 2 wk after bloom stages reduced yield when compared with the weed-free control. Although it is a legal application, growers should use caution when applying glufosinate to WideStrike cotton because the manufacturer of glufosinate and the manufacturer of WideStrike cotton do not recommend this application. However, where GR Palmer amaranth is present, these applications may be necessary.

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Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

Section: Section I -Biochemistry and Physiology Of The Herbicide In Nmentioning

confidence: 99%

Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

Section: Section II -Metabolism Of Glufosinate-ammonium In Geneticallmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Barnett

Culpepper²,

York

et al. 2015

Weed technol.

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Metabolism of the herbicide glufosinate-ammonium in plant cell cultures of transgenic (rhizomania-resistant) and non-transgenic sugarbeet (Beta vulgaris), carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium)

Müller

Zumdick²,

Schuphan

et al. 2001

Pest. Manag. Sci.

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The metabolism of the herbicide glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) glufosinate, L-glufosinate, and D-glufosinate, as well as the metabolites N-acetyl L-glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from glufosinate or L-glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea.

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Soybean

Tallman

Zhang

et al. 2008

Compendium of Transgenic Crop Plants

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Soybean is the most valuable grain legume crop, representing the primary source of protein and vegetable oil throughout the world. In the past decades, soybean crops have been improved significantly in agronomic traits such as yield, disease and pest resistance, and seed composition by both conventional and transgenic breeding methods. In this chapter, we describe the history of soybean development and distribution, trait improvement by conventional breeding, the advancement of transformation and transgenic traits, current and future soybean products, and new technologies in modern agricultural practices. New transgenic soybean products with stacking traits can further benefit human life, environment preservation, and global economic advancement. Once biotechnology has gained widespread acceptance, the miracle soybean will fully express its beauty.

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The Metabolites of the Herbicide L-Phosphinothricin (Glufosinate) (Identification, Stability, and Mobility in Transgenic, Herbicide-Resistant, and Untransformed Plants)

Cited by 75 publications

References 17 publications

Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Metabolism of the herbicide glufosinate-ammonium in plant cell cultures of transgenic (rhizomania-resistant) and non-transgenic sugarbeet (Beta vulgaris), carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium)

Soybean

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