The metabolism of γ-hydroxyglutamate in rat liver I. Enzymic synthesis of γ-hydroxy-α-ketoglutarate from pyruvate and glyoxylate

Biochemistry of Selected Amino Acids

“…An enzyme has been reported that condenses pyruvate and glyoxalate to form y-hydroxY-IX-ketoglutarate. (177) This product can be transaminated to y-hydroxyglutamate,(178) which may be reduced to hydroxyproline by systems analogous to those described for reduction of glutamate to proline. (179) …”

Alternate pathways of metabolism of short-chain fatty acids.

“…In animal tissues, L-hydroxyproline is catabolized to -y-hydroxy-L-glutamic acid (1,2,13). The further metabolism of this compound has been studied in several laboratories (15,54,(110)(111)(112)122, 123) and has been shown to proceed by transamination to D.-y-hydroxy-a-ketoglutarate (HKG), followed by cleavage to glyoxylate plus pyruvate. Both the transaminase and aldolase reactions are reversible.…”

Beef liver 2-Keto-4-hydroxyglutarate aldolase: Substrate stereospecificity and schiff base formation with pyruvate and glyoxylate

“…In previous studies, this enzyme was partially purified from rat liver extracts by Kuratomi and Fukunaga (1963), by Maitra and Dekker (18641, and more extensivety by Rosso and Adams (1966). The latter investigators reported that in the presence of NaBH4 the rat liver enzyme forms 4 -1ysine derivatives with both substrates, glyoxylate and pyruvate,…”