The metabolism of soluble carbohydrates related to chilling injury in peach fruit exposed to cold stress

Wang, Ke; Shao, Xingfeng; Gong, Yifu; Zhu, Yiwei; Wang, Hongfei; Zhang, Xinglong; Yu, Dandan; Yu, Fang; Qiu, Ziyun; Lu, Hao

doi:10.1016/j.postharvbio.2013.06.020

Cited by 128 publications

(96 citation statements)

References 48 publications

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“…During cold storage, the chilling-sensitive cultivar has lower levels of H 2 O 2 and higher membrane lipid unsaturation than does the chilling-resistant cultivar ). In our tests, loquat fruit stored at 5°C exhibited more firmness and higher H 2 O 2 and MDA levels, and was therefore more sensitive to CI than fruit stored at 0°C.This result is similar to that reported for peach fruits stored at 0°C and 5°C (Zhang et al 2011;Wang et al 2013). In contrast, Cai et al (2006) reported that a storage temperature of 5°C maintains higher fruit quality than lower (0°C) temperatures in ''Luoyangqing'' loquats.…”

Section: Determination Of Asa-gsh Cycle Content Levels and Enzyme Actsupporting

confidence: 87%

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Changes in soluble sugar metabolism in loquat fruit during different cold storage

Wei

Shao

2017

J Food Sci Technol

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To investigate soluble sugar metabolism and its relationship to chilling injury (CI), loquat fruits were stored at 0 and 5°C for 5 weeks. CI symptoms were monitored during storage, and soluble sugar content and related enzyme activities were measured. Enzymes activities and substrate levels in the ascorbate-glutathione (AsA-GSH) cycle were also determined. Fruit at 0°C had lower sucrose levels and higher levels of glucose and fructose than fruit maintained at 5°C, and also manifested less severe CI symptoms. Fruit at 0°C also exhibited increased activities of acid invertase, neutral invertase, sucrose phosphatesynthase and sucrose synthase. AsA-GSH cycle activity at 0°C was much higher than that at 5°C. Glucose levels correlated positively with levels of AsA and GSH and ascorbate peroxidase (APX) activity. The increased glucose levels are sufficient to account for the chilling tolerance of loquat fruit.

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Section: Determination Of Asa-gsh Cycle Content Levels and Enzyme Actsupporting

confidence: 87%

“…The supernatant was used for the AsA assay according to the method of Wang et al (2013). The results were expressed as mg/100 g FW.…”

Section: Determination Of Asa-gsh Cycle Content Levels and Enzyme Actmentioning

confidence: 99%

Changes in soluble sugar metabolism in loquat fruit during different cold storage

Wei

Shao

2017

J Food Sci Technol

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“…SE late varieties displayed increasing ascorbate peroxidase expression levels under cold storage and the ROSMETER ‘ascorbate peroxidase mutant signature’ genes (Rosenwasser et al ) were kept under regular levels (Figs D and S4). Results from Wang et al () support that the ascorbate‐glutathione cycle is more active upon chilling injury‐inducing conditions.…”

Section: Discussionmentioning

confidence: 94%

“…Exposure to chilling has a dramatic effect on the plants gene expression (Tattersall et al ), sucrose metabolism (Wang et al ), architecture and growth (Patel and Franklin , Xia et al ) and membrane fluidity (Zhang and Tian ). As in other species, P. persica fruit cold exposure seems to trigger an acclimation program (Pons et al ), with the induction of C‐repeat‐binding factors (CBFs) genes being beneficial for the fruit postharvest quality upon cold storage (Liang et al ).…”

Section: Introductionmentioning

confidence: 99%

A Prunus persica genome‐wide RNA‐seq approach uncovers major differences in the transcriptome among chilling injury sensitive and non‐sensitive varieties

Nilo-Poyanco

Vizoso

Sanhueza

et al. 2018

Physiologia Plantarum

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Chilling injury represents a major constrain for crops productivity. Prunus persica, one of the most relevant rosacea crops, have early season varieties that are resistant to chilling injury, in contrast to late season varieties, which display chilling symptoms such as mealiness (dry, sandy fruit mesocarp) after prolonged storage at chilling temperatures. To uncover the molecular processes related to the ability of early varieties to withstand mealiness, postharvest and genome-wide RNA-seq assessments were performed in two early and two late varieties. Differences in juice content and ethylene biosynthesis were detected among early and late season fruits that became mealy after exposed to prolonged chilling. Principal component and data distribution analysis revealed that cold-stored late variety fruit displayed an exacerbated and unique transcriptome profile when compared to any other postharvest condition. A differential expression analysis performed using an empirical Bayes mixture modeling approach followed by co-expression and functional enrichment analysis uncover processes related to ethylene, lipids, cell wall, carotenoids and DNA metabolism, light response, and plastid homeostasis associated to the susceptibility or resistance of P. persica varieties to chilling stress. Several of the genes related to these processes are in quantitative trait loci (QTL) associated to mealiness in P. persica. Together, these analyses exemplify how P. persica can be used as a model for studying chilling stress in plants.

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“…The rate of penetration was 1 mm s −1 with a final penetration depth of 10 mm. An IB index was used to evaluate the degree of flesh browning based on a previous study (Wang et al ) and was calculated on the basis of IB rating using the following formula: IB index =100% × Σ[(internal browning scale) × (number of fruit with that internal browning scale)] / [4 × total number of fruit evaluated]. The rating scale was 0 = 0% flesh surface area affected, 1 = 1–25% area affected, 2 = 26–50% area affected, 3 = 51–70% area affected and 4 = 76–100% area affected.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic and metabolic analyses provide new insights into chilling injury in peach fruit

Wang

Zhang

et al. 2017

Plant Cell & Environment

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Low temperature conditioning (LTC) alleviates peach fruit chilling injury but the underlying molecular basis is poorly understood. Here, changes in transcriptome, ethylene production, flesh softening, internal browning and membrane lipids were compared in fruit maintained in constant 0 °C and LTC (pre-storage at 8 °C for 5 d before storage at 0 °C). Low temperature conditioning resulted in a higher rate of ethylene production and a more rapid flesh softening as a result of higher expression of ethylene biosynthetic genes and a series of cell wall hydrolases. Reduced internal browning of fruit was observed in LTC, with lower transcript levels of polyphenol oxidase and peroxidase, but higher lipoxygenase. Low temperature conditioning fruit also showed enhanced fatty acid content, increased desaturation, higher levels of phospholipids and a preferential biosynthesis of glucosylceramide. Genes encoding cell wall hydrolases and lipid metabolism enzymes were coexpressed with differentially expressed ethylene response factors (ERFs) and contained ERF binding elements in their promoters. In conclusion, LTC is a special case of cold acclimation which increases ethylene production and, operating through ERFs, promotes both softening and changes in lipid composition and desaturation, which may modulate membrane stability, reducing browning and contributing to alleviation of peach fruit chilling injury.

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The metabolism of soluble carbohydrates related to chilling injury in peach fruit exposed to cold stress

Cited by 128 publications

References 48 publications

Changes in soluble sugar metabolism in loquat fruit during different cold storage

Changes in soluble sugar metabolism in loquat fruit during different cold storage

A Prunus persica genome‐wide RNA‐seq approach uncovers major differences in the transcriptome among chilling injury sensitive and non‐sensitive varieties

Transcriptomic and metabolic analyses provide new insights into chilling injury in peach fruit

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