The Metabolism of Iron by Nitrogen-Fixing Rhizospheric Bacteria

Barton, Larry L.; Johnson, G. V.; Bishop, Yvonne M. M.

doi:10.1007/1-4020-4743-6_9

Cited by 10 publications

(14 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Iron transport is especially important in nitrogen fixing bacteria [40] because it increases fitness in the competitive rhizosphere [41,42]. Rhizobium leguminosarum (a symbiotic nitrogen fixing species) contains a fhuDCB operon for siderophore uptake [43].…”

Section: Resultsmentioning

confidence: 99%

“…Iron acquisition is important in bacteria, both as a requirement for growth and as an environmental signal that regulates gene expression [ 39 ]. Iron transport is especially important in nitrogen fixing bacteria [ 40 ] because it increases fitness in the competitive rhizosphere [ 41 , 42 ]. Rhizobium leguminosarum (a symbiotic nitrogen fixing species) contains a fhuDCB operon for siderophore uptake [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…The presence of Fe siderophores and transporter genes in non pathogenic, non-nitrogen fixing O. carboxidovorans reflects its similarity to nitrogen fixing symbiotic bacteria. The presence of these genes in O. carboxidovorans also indicates that its natural environment, like most natural environments, is restricted in iron [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Complete genome and comparative analysis of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5

et al. 2010

View full text Add to dashboard Cite

BackgroundOligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium capable of utilizing CO (carbon monoxide) and fixing CO2 (carbon dioxide). We previously published the draft genome of this organism and recently submitted the complete genome sequence to GenBank.ResultsThe genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5 consists of a 3.74-Mb chromosome and a 133-kb megaplasmid that contains the genes responsible for utilization of carbon monoxide, carbon dioxide, and hydrogen. To our knowledge, this strain is the first one to be sequenced in the genus Oligotropha, the closest fully sequenced relatives being Bradyrhizobium sp. BTAi and USDA110 and Nitrobacter hamburgiensis X14. Analysis of the O. carboxidovorans genome reveals potential links between plasmid-encoded chemolithoautotrophy and chromosomally-encoded lipid metabolism. Comparative analysis of O. carboxidovorans with closely related species revealed differences in metabolic pathways, particularly in carbohydrate and lipid metabolism, as well as transport pathways.ConclusionOligotropha, Bradyrhizobium sp and Nitrobacter hamburgiensis X14 are phylogenetically proximal. Although there is significant conservation of genome organization between the species, there are major differences in many metabolic pathways that reflect the adaptive strategies unique to each species.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Complete genome and comparative analysis of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In the context of global strategies for siderophore biosynthesis, we have been interested in the timing of biological N -oxygenation verses N -acylation and the fate of the resulting hydroxamate, including cyclotrimerization to trilactone macrocyclic scaffolds. A close relative to the A. fumigatus siderophore fusarinine is the trihydroxamate vicibactin, , produced by the N 2 -fixing, pea symbiotic bacterium Rhizobium leguminosarum . Although both siderophores are derived from similarly elaborated monomers, vicibactin harbors an additional degree of complexity in the d -isomer of Orn.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Tailoring of Ornithine in the Biosynthesis of the Rhizobium Cyclic Trihydroxamate Siderophore Vicibactin

2009

View full text Add to dashboard Cite

To acquire iron, the N 2 -fixing, symbiotic bacterium Rhizobium sp. produce the cyclic trihydroxamate siderophore vicibactin, containing a 30-membered tri-lactone scaffold. Herein we report the overproduction and purification of the six proteins VbsACGOLS in the bacterial host Escherichia coli and the reconstitution of the biosynthesis of vicibactin from primary metabolites. The flavoprotein VbsO acts as a pathway-initiating L-ornithine N 5 -hydroxylase, followed by VbsA which transfers (R)-3-hydroxybutyryl-from the CoA thioester to N 5 -hydroxyornithine to yield N 5 -((R)-3-hydroxybutyryl)-N 5 -hydroxy-L-ornithine. VbsL is a PLP-dependent epimerase acting at C 2 of the 10 atom monomer unit. VbsS, a nonribosomal peptide synthetase free standing module, then activates N 5 -((R)-3-hydroxybutyryl)-N 5 -hydroxy-D-ornithine as the AMP anhydride on the way to cyclotrimerization to the vicibactin scaffold. The last step, tris-acetylation of the C 2 amino group of desacetyl-D-vicibactin to the mature siderophore vicibactin is catalyzed distributively by VbsC, using three molecules of acetyl-CoA.

show abstract

“…Kobayashi [ 23 ] found Mg deficiency caused Fe-responsive gene increase, and it seems that Fe has more opportunities to synthesize ferritin under the condition of Mg shortage. Moreover, ferritin is involved in thylakoid synthesis and photosynthesis [ 24 ]. Thus Fe-deficiency will cause thylakoid growth to stagnate, and decrease the densities of chlorophyll a and b, P700, and cytochrome f [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Iron Source and Medium pH on Growth and Development of Sorbus commixta In Vitro

Xiao

Park

Guo

et al. 2020

IJMS

View full text Add to dashboard Cite

Sorbus commixta is a valuable hardwood plant with a high economical value for its medicinal and ornamental qualities. The aim of this work was to investigate the effects of the iron (Fe) source and medium pH on the growth and development of S. commixta in vitro. The Fe sources used, including non-chelated iron sulfate (FeSO4), iron ethylenediaminetetraacetic acid (Fe-EDTA), and iron diethylenetriaminepentaacetic acid (Fe-DTPA), were supplemented to the Multipurpose medium with a final Fe concentration of 2.78 mg·L−1. The medium without any supplementary Fe was used as the control. The pH of the agar-solidified medium was adjusted to either 4.70, 5.70, or 6.70. The experiment was conducted in a culture room for six weeks with 25 °C day and night temperatures, and a 16-h photoperiod with a light intensity of 50 mmol·m−2·s−1 photosynthetic photon flux density (PPFD). Both the Fe source and pH affected the growth and development of the micropropagated plants in vitro. The leaves were greener in the pH 4.70 and 5.70 treatments. The tissue Fe content decreased with the increase of the medium pH. The leaf chlorophyll content was similar between plants treated with FeSO4 and those with Fe-EDTA. The numbers of the shoots and roots of plantlets treated with FeSO4 were 2.5 and 2 times greater than those of the control, respectively. The fresh and dry weights of the shoot and the root were the greatest for plants treated with Fe-EDTA combined with pH 5.70. The calcium, magnesium, and manganese contents in the plantlets increased in the pH 5.70 treatments regardless of the Fe source. Supplementary Fe decreased the activity of ferric chelate reductase. Overall, although the plantlets absorbed more Fe at pH 4.70, Fe-EDTA combined with pH 5.70 was found to be the best for the growth and development of S. commixta in vitro.

show abstract

The Metabolism of Iron by Nitrogen-Fixing Rhizospheric Bacteria

Cited by 10 publications

References 50 publications

Complete genome and comparative analysis of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5

Complete genome and comparative analysis of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5

Enzymatic Tailoring of Ornithine in the Biosynthesis of the Rhizobium Cyclic Trihydroxamate Siderophore Vicibactin

Effect of Iron Source and Medium pH on Growth and Development of Sorbus commixta In Vitro

Contact Info

Product

Resources

About