The Metabolism of Indole-3-acetic Acid by Geranium Stem Callus Cultures

Troxler, Robert F.; Hamilton, Robert H.

doi:10.1104/pp.40.2.400

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…A variant carrot strain GD‐31 derived from GD‐3 has a weak activity of IAA degradation and was not synchronized in IAA medium. Troxler and Hamilton (1964) have reported that in geranium callus culture the IAA supplied in the medium was gradually metabolized and became undetectable after about 90 days at which time growth stopped. Unlike geranium callus, the culture solution of the carrot cells did not display IAA degrading activity.…”

Section: Discussionmentioning

confidence: 99%

Partial Synchronization of Carrot Cell Culture by Auxin Deprivation

Nishi

Kato

Takahashi

et al. 1977

Physiologia Plantarum

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A strain of carrot cells (Daucus carota cv. Kintoki) grew exponentially in the presence of 2,4‐dichlorophenoxyacetic acid (2,4‐D, 1 mg/1) with a doubling time of about 2 days. When those cells were transferred to a medium lacking 2,4‐D, they continued to grow at almost the same rate for about a week. When the cells were again transferred to the auxin‐free medium, the rate of cell division gradually decreased. After the cell division had ceased, cells were returned to the ordinary 2,4‐D medium. A burst of cell divisions occurred after about 2 days. Timing of DNA synthesis and of mitosis suggested that the cells had been arrested at G1 phase. In a medium containing indoleacetic acid instead of 2,4‐D, the auxin was rapidly degraded and the culture was similarly synchronized as in the auxin‐omitted medium.

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Section: Discussionmentioning

confidence: 99%

Partial Synchronization of Carrot Cell Culture by Auxin Deprivation

Nishi

Kato

Takahashi

et al. 1977

Physiologia Plantarum

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“…Why is tryptophol active over the same range of concentrations as IAA as far as the initial concentrations in the media are concerned, and why should more genotypes respond to IAA formed from tryptophol than to IAA applied de novo? IAA included in the nutrient medium is almost certainly metabolized by pumpkin hypocotyl sections, as it is by other plant tissues (Reinert et al 1957, Lipetz and Gaiston 1959, Straus and Campbell 1963, Straus and Gerding 1963, Troxler and Hamilton 1965, Epstein aod Lavee 1975. Is it that the explant can respond only when a sufficient amount of this atixin is left during the phases of cailus or root induction?…”

Section: Response Of Primary Explantsmentioning

confidence: 99%

Induction of embryogenic callus in Cucurbita pepo hypocotyl explants by indole‐3‐ethanol and its sugar conjugates

Jelaska¹,

Magnus²,

Seretin³

et al. 1985

Physiologia Plantarum

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Hypocotyl explants of pumpkin (Cucurbita pepo L.) were inoculated aseptically on solid media containing Murashige and Skoog (MS) macro‐ and micronutrients, organic supplements, glucose as a carbon source, and indoleacetic acid (IAA) or one of its possible metabolic precursors, i.e. indole‐3‐ethanol [3‐(2‐hydroxyethyl)‐indole, tryptophol], 2‐(indol‐3‐yl)‐ethyl α‐L‐arabinopyranoside (tryptophol arabinoside), and 2‐(indol‐3‐yl)‐ethyl β‐D‐glucopyranoside (tryptophol glucoside). Embryogenic callus and adventitious roots developed on the explants, the response depending on the source of auxin, its concentration, and endogenous, possibly genetic, factors. Hypocotyl sections did not form embryogenic tissues and did not survive on media which did not contain an auxin or auxin precursors. Fewer explants responded to IAA than to indole‐3‐ethanol. Its arabinopyranoside was even more effective, while only a few hypocotyl sections proliferated in the presence of the corresponding glucopyranoside. Embryogenic callus induced by any of the four above compounds could be subcultured on media containing the same source of auxin. Selected callus lines have been maintained for more than three years and have continued to form embryoids. Indole‐3‐ethanol and IAA were suitable for clonal propagation of regenerated plantlets by axillary and apical bud culture.

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