The metabolism of benzyladenine in Spathiphyllum floribundum ‘Schott Petite’ in relation to acclimatisation problems

Werbrouck, Stefaan; Jeugt, Bruno van der; Dewitte, Walter; Prinsen, Els; Onckelen, Henri A. Van; Debergh, Pierre

doi:10.1007/bf00232734

Cited by 99 publications

(63 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For the ecological purpose of maintaining genetic diversity and achieving cost-effective commercial micropropagation, the efficient in vitro production and ex vitro acclimatization of a large number of sea oats genotypes is essential. Benzyladenine (BA), the most widely used cytokinin incorporated into culture media to promote axillary shoot production, often has deleterious effects on subsequent rooting and ex vitro acclimatization (Pospíšilová et al 1992;Werbrouck et al 1995). Currently, BA is the only effective cytokinin that promotes in vitro shoot production of sea oats (Philman and Kane 1994).…”

Section: Introductionmentioning

confidence: 98%

“…Currently, BA is the only effective cytokinin that promotes in vitro shoot production of sea oats (Philman and Kane 1994). Werbrouck et al (1995) correlated reductions in rooting and acclimatization of Spathiphyllum floribundum (peace lily) shoots produced on BA-supplemented medium with the accumulation of the BA metabolite 9-B-glucopyransosyl-benzyladenine (9G-BA) at the base of plantlets in vitro. Hydroxylated analogs of BA have been proposed as alternative cytokinin substitutes to BA in plant tissue systems (Werbrouck et al 1996;Strnad 1997).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes

et al. 2009

View full text Add to dashboard Cite

Benzyladenine (BA) is the only cytokinin to effectively induce shoot multiplication in vitro between genotypes of the important dune grass species Uniola paniculata (sea oats). However, a significant genotypespecific negative carryover effect of BA on ex vitro acclimatization has been observed. In the present study, the effects of multiplication media supplemented with metatopolin (mT), a BA-analog, BA or no plant growth regulator, were compared on in vitro multiplication, rooting and ex vitro acclimatization using easy-and difficult-to-acclimatize sea oats genotypes. Both genotypes exhibited similar in vitro shoot dry weight, number of harvestable shoots and percent rooting when cultured under standard conditions (with 2.2 lM BA) or with an equimolar concentration of mT. In addition, both genotypes exhibited similar ex vitro leaf length and shoot production under these two culture conditions. However, ex vitro acclimatization of rooted microcuttings of the difficult-to-acclimatize genotype significantly increased when produced on shoot multiplication medium containing mT rather than BA. Metatopolin concentrations 10 lM or greater were inhibitory to in vitro rooting and acclimatization ex vitro of both genotypes. Nevertheless, survival of the difficult-to-acclimatize genotype was significantly greater when cultured in the presence of 2.2 lM-30 lM mT, compared to 2.2 lM BA. Therefore, a potential solution to overcome the detrimental BA carryover effect on ex vitro survival in sea oats is the substitution of BA with 2.2 lM mT for Stage II shoot multiplication. Use of mT may provide an efficient method to ensure in vitro propagation of a large number of diverse sea oats genotypes for dune restoration.

show abstract

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes

et al. 2009

View full text Add to dashboard Cite

show abstract

“…It was also shown that another naturally occurring aromatic cytokinin, mT, due to its faster degradation in plant tissue, did not have negative effect on rhizogenic capacity [Werbrouck et al 1995, Wojtania et al 2011. In our study, however, neither lowering the cytokinin concentration nor replacement of BA to mT, which is BA derivative, positively influenced rooting.…”

Section: Discussionmentioning

confidence: 99%

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Podwyszyńska

Cieślińska

2018

Acta Sci.Pol.Hortorum Cultus

View full text Add to dashboard Cite

For breeding purposes, the number of neo-tetraploids of apple cultivars have been derived by in vitro technique. The first attempts at rooting and acclimatization of tetraploid shoots failed. The aim of the study was to develop an effective method for rooting microcuttings of apple neo-tetraploids. In the first stage of the study, in vitro rooting method was optimized for the shoots of diploid donor cultivars. Shoots were rooted on Murashige and Skoog [1962] (MS) medium with a reduced content of nitrogen, in the presence of auxins alone or in combination (indole-3-butyric acid -IBA, 1-naphthaleneacetic acid -NAA and indole--3-acetic acid -IAA) with addition of putrescine, arginine or ornithine. The compounds were applied continuously for 25 days (one-step rooting system) or for seven days with subsequent transplanting shoots onto a medium without these compounds (two-step rooting method). Tetraploid microcuttings of the cultivars 'Free Redstar', 'Gala Must', 'Pinova' and 'Redchief' were evaluated for rooting on the selected medium considered optimal for their diploid counterparts. The shoots of all diploid apple scion cultivars had low rooting capacity. IBA alone poorly stimulated root formation. Significant improvement of rooting to 60-80% was achieved through the application of auxins, 2.5 µM IBA or 1.3 µM NAA combined with 5 µM IAA and 50 µM putrescine in the two-step rooting system with darkness and increased temperature of 26°C during seven-day induction phase. The replacement of benzyladenine (BA) by meta-Topolin (m-T) in the last multiplication subculture influenced positively shoot acclimatization. Tetraploids had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts.

show abstract

“…The side effects of benzyladenine may be due to N 7 -and N 9 -glycosilation or alanin conjugation resulting in biologically inactive but chemically very stable derivatives. The slow release of benzyladenine from its derivatives might cause the inhibition of rooting as proved in other plant species (Werbrouck et al, 1995).…”

Section: Introductionmentioning

confidence: 92%

High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues

Magyar-Tábori

Dobránszki

Jámbor-Benczúr

2002

Acta Agronomica Hungarica

View full text Add to dashboard Cite

The in vitro shoot multiplication of apple cv. Jonagold was tested on media containing benzyladenine, benzyladenine riboside or meta-topolin in different concentrations (from 0.0 to 5.0 mg l -1 ). The optimal concentration for the best multiplication varied according to the type of cytokinin. The highest multiplication rate (on average 6.9 and 5.9 new shoots per explant) was achieved using 5.0 mg l -1 metatopolin or 2.0 mg l -1 benzyladenine riboside. The longest shoots were formed on media containing benzyladenine riboside at a concentration of 0.5 mg l -1 . The length of newly developed shoots was strongly suppressed by high concentrations of different cytokinins, but the suppression effect of a high concentration of meta-topolin on shoot length was less than that of benzyladenine or benzyladenine riboside. In this study meta-topolin and benzyladenine riboside proved to be effective cytokinins to induce adequate shoot proliferation, while benzyladenine was the least active cytokinin.

show abstract

The metabolism of benzyladenine in Spathiphyllum floribundum ‘Schott Petite’ in relation to acclimatisation problems

Cited by 99 publications

References 10 publications

Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes

Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues

Contact Info

Product

Resources

About