The Metabolic Profile of Anchusa officinalis L. Differs According to Its Associated Arbuscular Mycorrhizal Fungi

Tsiokanos, E; Cartabia, Annalisa; Tsafantakis, Nikolaos; Lalaymia, Ismahen; Termentzi, Aikaterini; Miguel, Maria; Declerck, Stéphane; Fokialakis, Nikolas

doi:10.3390/metabo12070573

Cited by 10 publications

(7 citation statements)

References 60 publications

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“…In Experiment 2, the objective was to evaluate the production of A/Sd and the expression of genes involved in the biosynthetic pathways of A/Sd in A. tinctoria roots colonized (later abbreviated for fluency as M irr , M aggreg , M Rhiz , and M Sept ) or not (NM) with R. irregularis MUCL 41833, R. aggregatus MUCL 49408, the native R. irregularis and S. viscosum and grown for 9 days in the S-H cultivation system. This harvesting time was based on the study of Tsiokanos et al (2022) where different AMF strains were associated to A. officinalis , and plants were harvested after 9 days of growth in the S-H cultivation system. Interesting results were obtained following this harvesting time and therefore considered in the present study.…”

Section: Methodsmentioning

confidence: 99%

“…(i.e., shoot and root tissues as well as exudates in the nutrient solution), another important Boraginaceae plant, under a semi-hydroponic (S-H) cultivation system (Cartabia et al, 2021). A similar study, conducted with A. officinalis associated with different AMF species (R. irregularis MUCL 41833, Rhizophagus intraradices MUCL 49410, Rhizophagus clarus MUCL 46238, and R. aggregatus MUCL 49408), demonstrated that specific symbiotic associations can affect the production of 10.3389/fmicb.2023.1216029 bioactive compounds differently in the same host (Tsiokanos et al, 2022).…”

Section: Introductionmentioning

confidence: 97%

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Arbuscular mycorrhizal fungi impact the production of alkannin/shikonin and their derivatives in Alkanna tinctoria Tausch. grown in semi-hydroponic and pot cultivation systems

et al. 2023

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IntroductionAlkanna tinctoria Tausch. is a medicinal plant well-known to produce important therapeutic compounds, such as alkannin/shikonin and their derivatives (A/Sd). It associates with arbuscular mycorrhizal fungi (AMF), which are known, amongst others beneficial effects, to modulate the plant secondary metabolites (SMs) biosynthesis. However, to the best of our knowledge, no study on the effects of AMF strains on the growth and production of A/Sd in A. tinctoria has been reported in the literature.MethodsHere, three experiments were conducted. In Experiment 1, plants were associated with the GINCO strain Rhizophagus irregularis MUCL 41833 and, in Experiment 2, with two strains of GINCO (R. irregularis MUCL 41833 and Rhizophagus aggregatus MUCL 49408) and two native strains isolated from wild growing A. tinctoria (R. irregularis and Septoglomus viscosum) and were grown in a semi-hydroponic (S-H) cultivation system. Plants were harvested after 9 and 37 days in Experiment 1 and 9 days in Experiment 2. In Experiment 3, plants were associated with the two native AMF strains and with R. irregularis MUCL 41833 and were grown for 85 days in pots under greenhouse conditions. Quantification and identification of A/Sd were performed by HPLC-PDA and by HPLC-HRMS/MS, respectively. LePGT1, LePGT2, and GHQH genes involved in the A/Sd biosynthesis were analyzed through RT-qPCR.ResultsIn Experiment 1, no significant differences were noticed in the production of A/Sd. Conversely, in Experiments 2 and 3, plants associated with the native AMF R. irregularis had the highest content of total A/Sd expressed as shikonin equivalent. In Experiment 1, a significantly higher relative expression of both LePGT1 and LePGT2 was observed in plants inoculated with R. irregularis MUCL 41833 compared with control plants after 37 days in the S-H cultivation system. Similarly, a significantly higher relative expression of LePGT2 in plants inoculated with R. irregularis MUCL 41833 was noticed after 9 versus 37 days in the S-H cultivation system. In Experiment 2, a significant lower relative expression of LePGT2 was observed in native AMF R. irregularis inoculated plants compared to the control.DiscussionOverall, our study showed that the native R. irregularis strain increased A/Sd production in A. tinctoria regardless of the growing system used, further suggesting that the inoculation of native/best performing AMF is a promising method to improve the production of important SMs.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Arbuscular mycorrhizal fungi impact the production of alkannin/shikonin and their derivatives in Alkanna tinctoria Tausch. grown in semi-hydroponic and pot cultivation systems

et al. 2023

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“…According to the relative abundance of the fragment ions and their retention times, they were identified as 3-and 5-caffeoylquinic acids. In addition, one dicaffeoylquinic acid isomer was also detected with a deprotonated molecular [2] C, [6,7] EV, [24,25] EI, [7,26] O [27] A, [28] EV [29] PA3 Caffeic acid 12.5 226, 298sh, 328 179 135 C, EV A, [2] EV, [24] EI, [26] O [27 [2] C, [6] EI, [26] O [27 A [2] , C [6,7] , EV [24,25] , EI [7,26] , O [27] [2] C, [6] EI, [26] ion at m/z 515 and product ions at m/z 353 (loss of caffeoyl moiety) and at m/z 173 (quinic acid-H 2 O).…”

Section: Characterization and Distribution Of Phenolic Compoundsmentioning

confidence: 99%

Extraction, Distribution and Diversity of Phenolic Compounds in Most Widespread Boraginaceae Species from Macedonia

Станоева

Стефова

Matevski

2023

Chemistry & Biodiversity

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A systematic study of extraction efficiency of polyphenolic compounds from the most widespread Boraginaceae species was carried out. The optimal extraction was achieved with 50 % (V/V) methanol for phenolic acids and flavonoids, 0.2 % (V/V) HCl in 50 % (V/V) methanol for anthocyanins and pure water for flavan-3-ols. The distribution and diversity of polyphenolic compounds in plant material obtained from wild-growing Anchusa officinalis, Cynoglossum creticum Mill., Echium vulgare, Echium italicum, and Onosma heterophylla Griseb. species from Macedonia was also assessed. These widespread Boraginaceae species contain phenolic acid derivatives, flavonoids, flavan-3-ols and anthocyanins and in total 31 of them were identified, from which 22 were first identified in the representative species, and 6,8-di-C-glucosides of apigenin and luteolin were identified for the first time in Boraginaceae. The profiles of polyphenolic compounds for each sample were obtained and their phytochemical profile established. The potential for further bioactivity studies of Anchusa officinalis and Cynoglossum creticum containing up to 24577.05 μg/g and 14304.15 μg/g of total polyphenols were assumed to be highest, followed by Echium vulgare (from 6382.61 to 14114.33 μg/g), Onosma heterophylla (9463.97 μg/g) and Echium (4108.14 μg/g).

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“…These AMF-induced changes reflect the influence of AMF on invasive plants, potentially offering a partial explanation for the successful invasion of exotic plants. However, the precise mechanisms through which AMF affects invasive plants remain largely unknown, and the specificity of plant-fungus symbiosis outcomes further complicates the explanation of this mechanism [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Impacts of Arbuscular Mycorrhizal Fungi on Metabolites of an Invasive Weed Wedelia trilobata

Jiang,

Chen,

Zhang

et al. 2024

Microorganisms

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The invasive plant Wedelia trilobata benefits in various aspects, such as nutrient absorption and environmental adaptability, by establishing a close symbiotic relationship with arbuscular mycorrhizal fungi (AMF). However, our understanding of whether AMF can benefit W. trilobata by influencing its metabolic profile remains limited. In this study, Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was conducted to analyze the metabolites of W. trilobata under AMF inoculation. Metabolomic analysis identified 119 differentially expressed metabolites (DEMs) between the groups inoculated with AMF and those not inoculated with AMF. Compared to plants with no AMF inoculation, plants inoculated with AMF showed upregulation in the relative expression of 69 metabolites and downregulation in the relative expression of 50 metabolites. AMF significantly increased levels of various primary and secondary metabolites in plants, including amino acids, organic acids, plant hormones, flavonoids, and others, with amino acids being the most abundant among the identified substances. The identified DEMs mapped 53 metabolic pathways, with 7 pathways strongly influenced by AMF, particularly the phenylalanine metabolism pathway. Moreover, we also observed a high colonization level of AMF in the roots of W. trilobata, significantly promoting the shoot growth of this plant. These changes in metabolites and metabolic pathways significantly affect multiple physiological and biochemical processes in plants, such as free radical scavenging, osmotic regulation, cell structure stability, and material synthesis. In summary, AMF reprogrammed the metabolic pathways of W. trilobata, leading to changes in both primary and secondary metabolomes, thereby benefiting the growth of W. trilobata and enhancing its ability to respond to various biotic and abiotic stressors. These findings elucidate the molecular regulatory role of AMF in the invasive plant W. trilobata and provide new insights into the study of its competitive and stress resistance mechanisms.

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The Metabolic Profile of Anchusa officinalis L. Differs According to Its Associated Arbuscular Mycorrhizal Fungi

Cited by 10 publications

References 60 publications

Arbuscular mycorrhizal fungi impact the production of alkannin/shikonin and their derivatives in Alkanna tinctoria Tausch. grown in semi-hydroponic and pot cultivation systems

Arbuscular mycorrhizal fungi impact the production of alkannin/shikonin and their derivatives in Alkanna tinctoria Tausch. grown in semi-hydroponic and pot cultivation systems

Extraction, Distribution and Diversity of Phenolic Compounds in Most Widespread Boraginaceae Species from Macedonia

Impacts of Arbuscular Mycorrhizal Fungi on Metabolites of an Invasive Weed Wedelia trilobata

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