The metabolic, circulatory, and bronchomotor responses of asthmatic children to epinephrine infusion

Lecks, Harold I.; Wood, David; Donsky, G. J.

doi:10.1016/0021-8707(69)90031-8

Cited by 9 publications

(1 citation statement)

References 22 publications

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“…Although these results are compat ible with increases in smooth contractile response, it is difficult to interpret these in vivo studies involving complex hormonal and reflex responses. There have been some studies in which there was no apparent change in beta-adrenergic response in asthmatic pa tients [7,18,33]. As an alternative method, many workers have studied the in vitro function of periph eral leukocytes from subjects with allergic asthma [19,22,24,27], Radioligand binding assays for studying the recep tors have been developed over the past several years, and are providing new insight about the status of the receptors [21,31].…”

Section: Introductionmentioning

confidence: 99%

Changes of Alpha<sub>1</sub>- and Beta-Adrenergic and Cholinergic Muscarinic Receptors in Guinea Pig Lung Sensitized with Ovalbumin

Mita¹,

Yui²,

Yasueda³

et al. 1983

Int Arch Allergy Immunol

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The effect of immunization of guinea pigs with ovalbumin on the number and affinity of alpha₁- and beta-adrenergic and cholinergic muscarinic receptors was studied in lung membranes by direct binding techniques using ³H-prazosin, l-³H-dihydroalprenolol and l-³H-quinuclidinyl benzilate. After immunization by intraperitoneal injection of ovalbumin to guinea pigs, the number and affinity of each receptor in sensitized animals were not significantly different from those of control animals. Sensitization of guinea pigs by an aerosol exposure with the antigen resulted in a decreased number of beta-adrenergic receptor (458 ± 29 vs. 687 ± 56 fmol/mg protein; p < 0.01), and an increased number of alpha₁-adrenergic receptor (36 ± 2 vs. 25 ± 2 fmol/mg protein; p < 0.01), but no change was observed in the number of muscarinic receptors, as compared with control animals. On the other hand, following a prolonged sensitization of guinea pigs with a low dose of the aerosolized antigen, the number of muscarinic receptors was significantly increased in the lung of sensitized animals (50 ± 2 vs. 42 ± 2 fmol/mg protein; p < 0.01); however, we found no significant differences between sensitized and normal animals in the number of alpha₁- and beta-adrenergic receptors. There were no different changes in the affinity of these receptors in all experiments.

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