The mesh skin graft—true expansion rate

Peeters, Rudi L.; Hubens, A

doi:10.1016/0305-4179(88)90047-2

Cited by 39 publications

(12 citation statements)

References 1 publication

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“…It has been reported that the meshed skin can be expended to cover wounds larger than the donor site. 35,36 Our results have demonstrated that 0.9 mm diameter MFUS is suitable size for large full-thickness skin wound healing without hurting the donor side tissues.…”

Section: Discussionmentioning

confidence: 68%

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Chang

Sun

et al. 2022

J Tissue Eng

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Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its application. This study shows a novel tissue engineering functional skin by carrying minimal functional unit of skin (MFUS) in 3D-printed polylactide-co-caprolactone (PLCL) scaffold and collagen gel (PLCL + Col + MFUS). MFUS is full-layer micro skin harvested from rat autologous tail skin. 3D-printed PLCL elastic scaffold has the similar mechanical properties with rat skin which provides a suitable environment for MFUS growing and enhances the skin wound healing. Four large full-thickness skin defects with 30 mm diameter of each wound are created in rat dorsal skin, and treated either with tissue engineering functional skin (PLCL + Col + MFUS), or with 3D-printed PLCL scaffold and collagen gel (PLCL + Col), or with micro skin islands only (Micro skin), or without treatment (Normal healing). The wound treated with PLCL + Col + MFUS heales much faster than the other three groups as evidenced by the fibroblasts migration from fascia to the gap between the MFUS dermis layer, and functional skin with hair follicles and sebaceous gland has been regenerated. The PLCL + Col treated wound heals faster than normal healing wound, but no skin appendages formed in PLCL + Col-treated wound. The wound treated with micro skin islands heals slower than the wounds treated either with tissue engineering skin (PLCL + Col + MFUS) or with PLCL + Col gel. Our results provide a new strategy to use autologous MFUS instead “seed cells” as the bio-resource of engineering skin for large full-thickness skin wound healing.

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Section: Discussionmentioning

confidence: 68%

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Chang

Sun

et al. 2022

J Tissue Eng

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“…The average expansion rates of skin grafts were not as high as the values claimed by the manufacturers of mesh devices. Previous studies reported that 1.5:1 meshed skin grafts expanded to 1.23 times or 1.27 ± 0.15 times, 1,2 and that 3:1 meshed skin grafts expanded to 1.59 ± 0.15 times 2,3 . The reason why the actual skin grafts do not achieve the claimed expansion rates may be due to the thickness of skin grafts and their elasticity.…”

Section: Discussionmentioning

confidence: 95%

“…Regarding expansion of meshed skin grafts, the measurement of skin grafts before and after the mesh processing revealed that mesh devices provided lower expansion rates compared with those claimed by manufactures. [1][2][3] In surgical procedures, the area of the donor sites to cover the target recipient sites depends not only on the expansion rate of mesh devices but also on shrinkage of harvested skin grafts and the shape of recipient sites. However, knowledge is lacking concerning the correlation, or coverage rate, between the area of donor sites and that of recipient sites covered by meshed grafts.…”

Section: Introductionmentioning

confidence: 99%

Calculation of practical skin donor area for meshed skin grafting in real‐world surgery

Kan

Takahagi

Kawai

et al. 2020

Dermatologic Therapy

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Meshed skin grafting is a common technique in operations to minimize surgery on the donor site area. However, the donor site area is empirically determined by surgeons due to the lack of a reliable formula to calculate the donor area required to cover a skin defect. To determine the minimal size for donor skin, the expansion rates of 1.5:1, 3:1, and 6:1 meshed skin graft and the area actually covered by them were investigated in real-world operations. About 51 patients who received 57 operations with meshed skin grafts were enrolled in this study. The average clinical coverage rates of area in cases in which 1.5:1, 3:1, or 6:1 meshed skin grafting was performed were 1.02, 1.29, or 2.18, respectively. Those rates were notably low when recipient sites were concave. The average expansion rates of 1.5:1, 3:1, and 6:1 meshed skin grafts were about 1.16, 1.61, and 2.32, respectively. These results indicate that the size of donor skin should be about 85%, 60%, and 45% size of the recipient site to achieve the target 1.5:1, 3:1, and 6:1 meshed skin graft, respectively. In addition, the donor area should be adjusted in consideration of the shape of the recipient sites.

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“…It has been reported that the meshed skin can be expended to cover wounds larger than the donor site. [28] However, it is difficult to keep all functional organs for full thickness skin wound regeneration if the size of the meshed skin is too small. Our results indicated that 1 mm diameter microskin particle is an MFUS for full thickness skin healing.…”

Section: Discussionmentioning

confidence: 99%

Fully Reduced HMGB1‐Containing Peptide‐Based Polyurethane Scaffold with Minimal Functional Unit of Skin (MFUS) Enhances Large and Deep Wounded Skin Healing

Chang

Sun

et al. 2021

Macromolecular Bioscience

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A novel peptide-based polymer is developed by lysine-diisocyanate (LDI), glycerol (Gly), and fully reduced HMGB1 (frHMGB1). This frHMGB1-LDI-Gly polymer either forms sponge-like foam (scaffold) or a hydrogel or a film under different reaction conditions. It degrades into nontoxic lysine, glycerol, and frHMGB1. The hydrogel glues tissues together and the glued tissues have strong mechanical properties. The film and scaffold provide the suitable environment for enhancing cell proliferation by releasing frHMGB1. The scaffold carries 1 mm diameter of full-thickness rat skin-island as a minimal functional unit of skin (MFUS) to treat large full thickness skin wounds, and the hydrogel glues the MFUS and scaffold with skin edges together (MFUS+Scaffold group). The scaffold treated wounds (Scaffold group) heal much faster than the wounds either treated with MFUS (MFUS group) or without treatment (Wound group). The MFUS+Scaffold treated wound regenerates more functional full-thickness skin with more hair follicles and sweat glands, higher CD146 and 𝜶-smooth muscle actin levels, more blood vessels and collagen productions, and less scar tissues when compared to the other three groups. The results demonstrate that the combination of frHMGB1-LDI-Gly polymer with MFUS provides a new tissue engineering approach for large full-thickness skin wound healing.

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The mesh skin graft—true expansion rate

Cited by 39 publications

References 1 publication

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Calculation of practical skin donor area for meshed skin grafting in real‐world surgery

Fully Reduced HMGB1‐Containing Peptide‐Based Polyurethane Scaffold with Minimal Functional Unit of Skin (MFUS) Enhances Large and Deep Wounded Skin Healing

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