The mercury resistance (<i>mer</i>) operon in a marine gliding flavobacterium,<i>Tenacibaculum discolor</i>9A5

Allen, Rachel C.; Tu, Yen Kuei; Nevarez, Michael J.; Bobbs, Alexander S.; Friesen, Joseph W.; Lorsch, Jon R.; Mccauley, John A.; Voet, Judith G.; Hamlett, Nancy V.

doi:10.1111/j.1574-6941.2012.01460.x

Cited by 17 publications

(7 citation statements)

References 66 publications

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“…However, the results in this paper demonstrate for the first time the significant presence of this group in the prokaryotic community of metal contaminated seawater. Flavobacteriacea was also a major component of the microbial community in strongly disturbed and metal-contaminated environments (Allen et al 2013;Wang et al 2015;Zouch et al 2018;Coclet et al 2019). Finally, the previous observation that Tenacibaculum and Formosa genera are positively selected in Cu-amended 6ext water as abundant and representative taxa from the most contaminated site 6extduring a one-month-long survey, is in agreement with the idea of a similar selective pressure in situ as that observed in our previous field study experiment (Coclet et al 2019).…”

Section: Copper Exposure Induced Changes In Prokaryotic Communitysupporting

confidence: 89%

Impacts of copper and lead exposure on prokaryotic communities from contaminated contrasted coastal seawaters: the influence of previous metal exposure

Coclet

Garnier

Durrieu

et al. 2020

FEMS Microbiology Ecology

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Our understanding of environmental factors controlling prokaryotic community is largely hampered by the large environmental variability across spatial scales (e.g. trace metal contamination, nutrient enrichment and physicochemical variations) and the broad diversity of bacterial pre-exposure to environmental factors. In this article, we investigated the specific influence of copper (Cu) and lead (Pb) on prokaryotic communities from the uncontaminated site, using mesocosm experiments. In addition, we studied how pre-exposure (i.e. life history) affects communities, with reference to previous metal exposure on the response of three prokaryotic communities to similar Cu exposition. This study showed a stronger influence of Cu contamination than Pb contamination on prokaryotic diversity and structure. We identified 12 and 34 bacterial families and genera, respectively, contributing to the significant differences observed in community structure between control and spiked conditions. Taken altogether, our results point toward a combination of direct negative responses to Cu contamination and indirect responses mediated by interaction with phytoplankton. These identified responses were largely conditioned by the previous exposure of community to contaminants.

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Section: Copper Exposure Induced Changes In Prokaryotic Communitysupporting

confidence: 89%

Impacts of copper and lead exposure on prokaryotic communities from contaminated contrasted coastal seawaters: the influence of previous metal exposure

Coclet

Garnier

Durrieu

et al. 2020

FEMS Microbiology Ecology

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“…In conclusion, our study suggested that the pool of merA loci among the arctic bacterial assemblages was diverse, mobile and represents both evolutionary recent and ancient lineages in the MerA phylogeny. The SOK62 merA gene was found to be part of an early mer operon in an isolate of the Bacteroidetes , a phylum where mercury resistance has only recently been described (Allen et al ., ). A phylogenetic analysis of this sequence together with other putative merA loci from Bacteroidetes suggested these loci to represent an early lineage in the mer phylogeny.…”

Section: Discussionmentioning

confidence: 97%

Mercuric reductase genes (merA) and mercury resistance plasmids in High Arctic snow, freshwater and sea-ice brine

Møller

Barkay

Hansen

et al. 2013

FEMS Microbiol Ecol

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Bacterial reduction in Hg(2+) to Hg(0) , mediated by the mercuric reductase (MerA), is important in the biogeochemical cycling of Hg in temperate environments. Little is known about the occurrence and diversity of merA in the Arctic. Seven merA determinants were identified among bacterial isolates from High Arctic snow, freshwater and sea-ice brine. Three determinants in Bacteriodetes, Firmicutes and Actinobacteria showed < 92% (amino acid) sequence similarity to known merA, while one merA homologue in Alphaproteobacteria and 3 homologues from Betaproteobacteria and Gammaproteobacteria were > 99% similar to known merA's. Phylogenetic analysis showed the Bacteroidetes merA to be part of an early lineage in the mer phylogeny, whereas the Betaproteobacteria and Gammaproteobacteria merA appeared to have evolved recently. Several isolates, in which merA was not detected, were able to reduce Hg(2+) , suggesting presence of unidentified merA genes. About 25% of the isolates contained plasmids, two of which encoded mer operons. One plasmid was a broad host-range IncP-α plasmid. No known incompatibility group could be assigned to the others. The presence of conjugative plasmids, and an incongruent distribution of merA within the taxonomic groups, suggests horizontal transfer of merA as a likely mechanism for High Arctic microbial communities to adapt to changing mercury concentration.

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“…5). A recent study reported the mercury resistance of a member of Tenacibaculum from marine Flavobacteriaceae (67). Moreover, flavobacteria made up 70% of a Cdresistant bacterial community in soil amended with sewage sludge (68) and have not been extensively studied but could be major components of the bacterial community in metal-polluted marine environments (67).…”

Section: Discussionmentioning

confidence: 99%

Response of Bacterioplankton Communities to Cadmium Exposure in Coastal Water Microcosms with High Temporal Variability

Wang

Zhang

Xiong

et al. 2015

Appl Environ Microbiol

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Multiple anthropogenic disturbances to bacterial diversity have been investigated in coastal ecosystems, in which temporal variability in the bacterioplankton community has been considered a ubiquitous process. However, far less is known about the temporal dynamics of a bacterioplankton community responding to pollution disturbances such as toxic metals. We used coastal water microcosms perturbed with 0, 10, 100, and 1,000 g liter ؊1 of cadmium (Cd) for 2 weeks to investigate temporal variability, Cd-induced patterns, and their interaction in the coastal bacterioplankton community and to reveal whether the bacterial community structure would reflect the Cd gradient in a temporally varying system. Our results showed that the bacterioplankton community structure shifted along the Cd gradient consistently after a 4-day incubation, although it exhibited some resistance to Cd at low concentration (10 g liter ؊1 ). A process akin to an arms race between temporal variability and Cd exposure was observed, and the temporal variability overwhelmed Cd-induced patterns in the bacterial community. The temporal succession of the bacterial community was correlated with pH, dissolved oxygen, NO 3 ؊ -N, NO 2 ؊ -N, PO 4 3؊ -P, dissolved organic carbon, and chlorophyll a, and each of these parameters contributed more to community variance than Cd did. However, elevated Cd levels did decrease the temporal turnover rate of community. Furthermore, key taxa, affiliated to the families Flavobacteriaceae, Rhodobacteraceae, Erythrobacteraceae, Piscirickettsiaceae, and Alteromonadaceae, showed a high frequency of being associated with Cd levels during 2 weeks. This study provides direct evidence that specific Cd-induced patterns in bacterioplankton communities exist in highly varying manipulated coastal systems. Future investigations on an ecosystem scale across longer temporal scales are needed to validate the observed pattern. In the last decades, heavy metal concentrations have increased significantly, reaching up to several mg liter Ϫ1 , in seriously polluted estuaries or harbors (1, 2) as a consequence of industrial discharge and river outflows. Cadmium (Cd) is one of the most toxic metals frequently detected in the coastal areas of China, and its presence exceeds the permissible environmental limit in several bays, including Bohai Bay and Jiaozhou Bay (3, 4), which can harm human health via seafood consumption because of its accumulative and carcinogenic properties (3,5,6). One of the major scientific challenges is to evaluate threats of pollution disturbances to the biodiversity. Bacterioplankton plays crucial roles in the mineralization of organic matter and nutrient cycling in marine ecosystems (7,8), and bacterioplankton community composition (BCC) quickly responds to anthropogenic disturbances such as chemical pollution, nutrient enrichment, and the introduction of antibiotics and pathogens (9). Therefore, bacterioplankton communities might be used to evaluate the ecological effect of pollution in a coastal environment.The resp...

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The mercury resistance (mer) operon in a marine gliding flavobacterium,Tenacibaculum discolor9A5

Cited by 17 publications

References 66 publications

Impacts of copper and lead exposure on prokaryotic communities from contaminated contrasted coastal seawaters: the influence of previous metal exposure

Impacts of copper and lead exposure on prokaryotic communities from contaminated contrasted coastal seawaters: the influence of previous metal exposure

Mercuric reductase genes (merA) and mercury resistance plasmids in High Arctic snow, freshwater and sea-ice brine

Response of Bacterioplankton Communities to Cadmium Exposure in Coastal Water Microcosms with High Temporal Variability

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