The membraneous type of lomasome (membranosome) in the hyphae ofAspergillus nidulans

Weisberg, S. H.; Turian, G.

doi:10.1007/bf01276612

Cited by 4 publications

(1 citation statement)

References 36 publications

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“…Some cells contained autophagic vacuoles or empty cytoplasm indicating a process of autolysis (Figure 3a). In some cells, lipid deposits (Figure 3b) and intracytoplasmic membranous structures (Figure 3c) appeared similar to membranosomes or lomasomes described by Weisberg and Turian (1974) and Wilsenach and Kessel (1965). After 24 h of incubation on water agar plates, CAs germinated forming hyphae and mycelia on the surface (Figure 4a); germinating blastospores with different length germ tubes were observed over the entire surface (Figure 4b).…”

Section: Description Of Germination and Sporulation Of Agr-f704 Aggregatessupporting

confidence: 75%

Three-dimensional cellular aggregates formed by Beauveria pseudobassiana in liquid culture with potential for use as a biocontrol agent of the African black beetle (Heteronychus arator)

et al. 2020

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Beauveria pseudobassiana formed three-dimensional aggregates of cells (CAs) in liquid culture. CAs were formed mainly by blastospores and conidia, distinct from microsclerotia formed through adhesion of hyphae. The formation, germination and sporulation of CAs were studied, as well as the pathogenicity of conidia produced from them against adults of black beetle. After 4 days of culture, CAs were formed, becoming compact and melanised after 10 days of incubation. Electron microscopy showed three-dimensional CAs averaging 431.65 µm in length with irregular shapes and rough surfaces, where cells were trapped within an extracellular matrix. CAs germinated after 2 days of incubation on agarplates producing hyphae and forming phialides and conidia after 4 days. Produced conidia caused 45% mortality of black beetle adults. CAs germination and sporulation on soil were directly correlated with soil moisture, reaching 80% and 100% germination on the surface of soil with 17% and 30% moisture, respectively. CAs maintained 100% germination after 2 years of storage under refrigeration. These CAs could have a similar function as microsclerotia in nature, acting as resistant structures able to protect internal cells and their ability to sporulate producing infective conidia, suggesting their potential to be used as bioinsecticides to control soil-dwelling insects.

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Section: Description Of Germination and Sporulation Of Agr-f704 Aggregatessupporting

confidence: 75%

Three-dimensional cellular aggregates formed by Beauveria pseudobassiana in liquid culture with potential for use as a biocontrol agent of the African black beetle (Heteronychus arator)

et al. 2020

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Ultrastructure of Diplodia maydis grown on selected synthetic media

1980

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Ultrastructural Alterations in Fusarium sambucinum and Heterobasidion annosum Treated with Aluminum Chloride and Sodium Metabisulfite

Avis¹,

Rioux²,

Simard³

et al. 2009

Phytopathology®

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Aluminum chloride (AlCl(3)) and sodium metabisulfite (Na(2)S(2)O(5)) have received increasing attention as antifungal agents for the control of plant diseases. In an effort to understand their toxic action on fungi, ultrastructural changes and membrane damage in Fusarium sambucinum (Ascomycota) and Heterobasidion annosum (Basidiomycota) in response to salt exposure was investigated using transmission electron microscopy. Conidial membrane damage was quantified using SYTOX Green stain, which only enters altered membranes. The results showed that mortality of the conidia was generally closely associated with SYTOX stain absorption in F. sambucinum treated with Na(2)S(2)O(5) and in H. annosum treated with AlCl(3) or Na(2)S(2)O(5), suggesting that these salts cause membrane alterations. For both fungi, ultrastructural alterations in conidia treated with AlCl(3) and Na(2)S(2)O(5) included membrane retraction, undulation, and invagination. At higher concentrations or exposure periods to the salts, loss of membrane integrity, cytoplasmic leakage, and cell rupture were observed. Ultrastructural alterations and increased SYTOX stain absorption in salt-treated conidia appear consistent with a mode of action where AlCl(3) and Na(2)S(2)O(5) alter membrane integrity and permeability.

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The membraneous type of lomasome (membranosome) in the hyphae ofAspergillus nidulans

Cited by 4 publications

References 36 publications

Three-dimensional cellular aggregates formed by Beauveria pseudobassiana in liquid culture with potential for use as a biocontrol agent of the African black beetle (Heteronychus arator)

Three-dimensional cellular aggregates formed by Beauveria pseudobassiana in liquid culture with potential for use as a biocontrol agent of the African black beetle (Heteronychus arator)

Ultrastructure of Diplodia maydis grown on selected synthetic media

Ultrastructural Alterations in Fusarium sambucinum and Heterobasidion annosum Treated with Aluminum Chloride and Sodium Metabisulfite

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