The Membrane Skeleton Controls Diffusion Dynamics and Signaling through the B Cell Receptor

Treanor, Bebhinn; Depoil, David; Gonzalez-Granja, Aitor; Barral, Patricia; Weber, Michele; Dushek, Omer; Bruckbauer, Andreas; Batista, Facundo D.

doi:10.1016/j.immuni.2009.12.005

Cited by 304 publications

(441 citation statements)

References 67 publications

Supporting

Mentioning

415

Contrasting

Unclassified

Order By: Relevance

“…In B cells, the cytoskeleton is a major target of BCR signalling pathways. Even in resting B cells, the structure of the cortical cytoskeleton depends on the presence of BCR signalling components, suggesting that there is a constant influence of tonic BCR signalling on actin dynamics 32 . Antigen binding to the BCR triggers dramatic remodelling of the cortical cytoskeleton that induces cell spreading, formation of the immune synapse, and gathering of antigen for endocytosis.…”

Section: [H1] Structure Of the Cortical Cytoskeletonmentioning

confidence: 99%

“…Single molecule tracking shows that the IgM BCR in resting B cells diffuses approximately at 0.05 μm 2 /s, a modest speed for a membrane protein 32,90 . In addition, up to twenty percent of the IgM molecules are completely immobile 90 .…”

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

“…In addition, up to twenty percent of the IgM molecules are completely immobile 90 . The IgD BCR is even slower, with the majority of the molecules immobile 32 . The diffusion of the BCR is particularly slow in areas of the membrane that are rich in actin and conversely, actin depolymerization increases BCR diffusion 32,60 .…”

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

“…The IgD BCR is even slower, with the majority of the molecules immobile 32 . The diffusion of the BCR is particularly slow in areas of the membrane that are rich in actin and conversely, actin depolymerization increases BCR diffusion 32,60 . The exact molecular mechanisms by which IgM and IgD BCRs interact with actin filaments are not known, although it is likely that these mechanisms involve the cytoplasmic tails of the BCR signalling subunits CD79A and CD79B 32 .…”

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

“…Instead, CD19 diffusion is limited because it is included in large protein clusters organized by the tetraspanin CD81 49 . Acute depolymerization of actin triggers calcium flux and other downstream pathways that involve the BCR, CD19 and CD81, indicating that by hindering BCR diffusion, the cortical actin limits the access of BCR-associated kinases to the CD19-CD81 complex 32,49 . These findings suggest that the actin cytoskeleton controls the resting state of B cells and that actin reorganization is necessary to activate CD19-dependent signalling in response to antigens.…”

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

See 4 more Smart Citations

Cytoskeletal control of B cell responses to antigens

Tolar

2017

Nat Rev Immunol

123

View full text Add to dashboard Cite

The actin cytoskeleton is essential for cell mechanics and has increasingly been implicated in the regulation of cell signalling. In B cells, the actin cytoskeleton couples extensively to B cell receptor (BCR) signalling pathways and its defects can either enhance or suppress B cell activation. Recent insights from single-cell imaging and biophysical techniques suggest that actin orchestrates BCR signalling at the plasma membrane through effects on protein diffusion, and that it regulates antigen discrimination through the biomechanics of immune synapses. These mechanical functions also play a role in the adaptation of B cell subsets to specialized tasks during antibody responses.3

show abstract

Section: [H1] Structure Of the Cortical Cytoskeletonmentioning

confidence: 99%

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

Section: [H1] Cytoskeletal Regulation Of Bcr Signallingmentioning

confidence: 99%

See 3 more Smart Citations

Cytoskeletal control of B cell responses to antigens

Tolar

2017

Nat Rev Immunol

123

View full text Add to dashboard Cite

show abstract

Immunological Synapse

Agüera-González¹,

Bouchet²,

Alcover³

2015

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

International audienceImmunological synapses are dynamically organised cell–cell interfaces formed between cells of the immune system. Different types of immunological synapses lead to distinct functional outcomes. Thus, T and B lymphocytes form immunological synapses with each other and with dendritic cells or macrophages, conveying mutual activation cues. Moreover, natural killer cells or cytotoxic T lymphocytes form synapses with tumour or infected cells delivering cytotoxic granules that destroy those anomalous cells. Immunological synapses are the result of an orchestrated cell polarisation process that involves cytoskeleton rearrangements, intracellular vesicle traffic and the clustering of receptors, adhesion molecules and signalling effectors, which together ensure the immunological synapse stability, its structure and function. Immunological synapses play key roles in immune responses, like T- and B-cell activation, and polarised secretion of cytokines or cytotoxic granules

show abstract

Cytoskeleton‐Induced Mesoscale Domains

Kalay¹,

Fujiwara²,

Kusumi

2011

Cellular Domains

View full text Add to dashboard Cite

DEFINITIONThe cell is much more than just a bag of protein juice. Indeed, many mechanisms exist in a living cell to keep its contents well organized. One of the most important apparatuses that the cell utilizes to organize the cytoplasm is the cytoskeleton. Therefore, the infl uence of the cytoskeleton on the two -dimensional fl uid of the plasma membrane is an interesting subject. In this chapter, we will focus on this issue, and review the literature about the membrane domains delimited by the part of the actin -based cytoskeleton that is closely opposed to the cytoplasmic surface of the plasma membrane. This part of the cytoskeleton is referred to as the membrane skeleton . Due to its close association with the cytoplasmic surface of the plasma membrane, the membrane -skeleton meshwork directly infl uences the functions of the plasma membrane. As a consequence of the membrane -skeleton meshwork, the plasma membrane is effectively partitioned into mesoscale domains, or compartments, with sizes varying between 30 and 250 nm (with the exception of the larger 750 -nm domain in the doubly nested compartments in normal rat kidney [NRK] cells; Fujiwara et al. 2002 ).We emphasize the characteristic size of these compartments, one to several hundred nanometers, that falls in the mesoscale, where collective dynamics of molecules play critical roles. At this size scale, the number of molecules in the system is insuffi cient for thermodynamics to hold, but is still too big to be tractable for quantum mechanics. In the plasma membrane, there are three types of major mesoscale domains ( meso domains): (1) membrane compartments delineated by the actin -based membrane skeleton; (2) raft domains, where specifi c proteins, glycosphingolipids, and cholesterol are concentrated; and (3) the protein oligomer domains. In this chapter, we will concentrate on the membraneskeleton -induced membrane compartments.Membrane lipids and proteins are both temporarily trapped in these membrane compartments with residency times between 1 ms and 1 second (Kusumi et al. 2005 ). Namely, the two key functional elements of the membrane are both infl uenced by the membrane skeleton, and a growing number of fi ndings support the involvement of the membrane skeleton in many membrane processes (

show abstract

The Membrane Skeleton Controls Diffusion Dynamics and Signaling through the B Cell Receptor

Cited by 304 publications

References 67 publications

Cytoskeletal control of B cell responses to antigens

Cytoskeletal control of B cell responses to antigens

Immunological Synapse

Cytoskeleton‐Induced Mesoscale Domains

Contact Info

Product

Resources

About