The Membrane Filter Technique for Estimating Numbers of Viable Bacteria

Abstract

were notched to facilitate withdrawal of the MF and were used repeatedly. A close-fitting sliding top, of the same dimensions as the bottom, afforded adequate 4Westinghouse Electric Corporation, Bloomfield, New Jersey. 6Trade name for a mixture of 90 per cent carbon dioxide and 10 per cent ethylene oxide, marketed by the Union Carbide and Chemical Company, New York, New York.

Enzymatic digestion method development for long-term stored chitinaceous planktonic samples

“…Therefore, the simplification for particle visualisation, counting and retrieval is key in the accurate study of microplastics. From the three types of filters compared in this study, grided membrane filters, traditionally used for microbiology applications (Wolochow 1958), were deemed as more appropriate for microplastics recovery from zooplankton samples as these facilitated the visibility and recovery of both microplastics and undigested material while retaining their physical integrity after manipulation with needles (Figure 1). Furthermore, the grid lines on the membrane surface eliminated eye fatigue and facilitated methodical filter examination.…”

Comparative Study of Membrane Filtration and Spread Plate Technique for Dialysis Water Analysis

“…A perfect spread plate technique will contain results with visible and isolated colonies of bacteria that are evenly distributed in the plate and are countable. This technique is commonly applied for microbial testing of foods or any other samples or to isolate and identify variety of microbial flora [5]. This study is taken up to compare both of these techniques to find out which is the best control to be used in dialysis water analysis.…”

“…Membrane filteration technique is an effective, accepted technique for testing fluid samples for microbiological contamination. Spread plate technique is used for viable plate counts, in which the total number of colony forming units on a single plate is enumerated [5]. By using the criteria of the AAMI, the present study is taken up to analyse the sensitivity of two different cultures technique i.e., spread plate and membrane filteration technique.…”