The Medium for Acceleration of <i>Mycobacterium tuberculosis</i> Growth

Abstract

This article has described the results of studies conducted on pre-culture samples in a modified environment Shkolnikova Dorozhkova at 37˚C during 24-48 hours. It was stated that this method helps grow Mycobacterium tuberculosis, which, when sown on the medium give rise visible colonies on the 5th-7th day. These data confirm the possibility to accelerate the growth of mycobacteria and shorten the terms of diagnosing tuberculosis by 3 or 4 times thanks to pre-culturing of the diagnostic material in the experimental medium. The original conception of the Mycobacterium tuberculosis development step-by-step was elaborated which was based on changeability of mycobacteria and prominent character of their development from division of the bacterial cells to the development of the maturated cells and further biological transformation under conditions of the surrounding factors. This conception gave the explanation of the integrated picture of the biological changeability of Mycobacterium tuberculosis. It was stated that the cocci-like, flask-like, grain-like, virus-like, filtrating forms, L-forms and other agent forms are the stages of the biological development of tuberculosis mycobateria which are not always identified by the routine methods of microbiological diagnostics implemented in the veterinary practice. Thus, the use of the developed method of diagnostics of tuberculosis in animals together with agglutination test, ELISA and PCR allow shortening the terms of primary diagnosis of tuberculosis in animals by bacteriological method by 5-7 days.