The Mechanochemistry of Endocytosis

Liu, Jian; Sun, Yidi; Drubin, David G.; Oster, George

doi:10.1371/journal.pbio.1000204

Cited by 221 publications

(262 citation statements)

References 56 publications

(138 reference statements)

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“…We also discovered that coronin1B and cofilin, proteins involved in the down-regulation of actin polymerization and F-actin severing, respectively, were recruited at later time points, again similar to yeast endocytosis [24,72,73]. Third, it was proposed that scission of endocytic invaginations in yeast is triggered by a PI-phosphatase that dephosphorylates PiP2 and thus induces a line tension in the membrane neck [74]. In mammalian cells the large GTPase dynamin is thought to execute scission [9,30], but, intriguingly, the recruitment of the PI-phosphatase synaptojaninb1 showed a recruitment trajectory similar to that of dynamin (and proteins of the NBAR module) and also peaked at scission (Figure 4).…”

Section: Similar To Yeast Mammalian Cme Has a Modular Designmentioning

confidence: 64%

“…In mammalian cells the large GTPase dynamin is thought to execute scission [9,30], but, intriguingly, the recruitment of the PI-phosphatase synaptojaninb1 showed a recruitment trajectory similar to that of dynamin (and proteins of the NBAR module) and also peaked at scission (Figure 4). Therefore, it is plausible that induction of a line tension also contributes to the mechanochemistry of scission in mammalian cells [74]. Finally, it was predicted that recruitment of F-BAR and BAR domain proteins should follow an ordered sequence dictated by their preference for different-curvature membrane tubules in vitro [75] and that recruitment should occur over a trajectory similar to that of actin polymerization [6,9,76].…”

Section: Similar To Yeast Mammalian Cme Has a Modular Designmentioning

confidence: 99%

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A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis

Perrais²,

2011

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Dual colour total internal reflection fluorescence microscopy is a powerful tool for decoding the molecular dynamics of clathrin-mediated endocytosis (CME). Typically, the recruitment of a fluorescent protein-tagged endocytic protein was referenced to the disappearance of spot-like clathrin-coated structure (CCS), but the precision of spot-like CCS disappearance as a marker for canonical CME remained unknown. Here we have used an imaging assay based on total internal reflection fluorescence microscopy to detect scission events with a resolution of ,2 s. We found that scission events engulfed comparable amounts of transferrin receptor cargo at CCSs of different sizes and CCS did not always disappear following scission. We measured the recruitment dynamics of 34 types of endocytic protein to scission events: Abp1, ACK1, amphiphysin1, APPL1, Arp3, BIN1, CALM, CIP4, clathrin light chain (Clc), cofilin, coronin1B, cortactin, dynamin1/ 2, endophilin2, Eps15, Eps8, epsin2, FBP17, FCHo1/2, GAK, Hip1R, lifeAct, mu2 subunit of the AP2 complex, myosin1E, myosin6, NECAP, N-WASP, OCRL1, Rab5, SNX9, synaptojanin2b1, and syndapin2. For each protein we aligned ,1,000 recruitment profiles to their respective scission events and constructed characteristic ''recruitment signatures'' that were grouped, as for yeast, to reveal the modular organization of mammalian CME. A detailed analysis revealed the unanticipated recruitment dynamics of SNX9, FBP17, and CIP4 and showed that the same set of proteins was recruited, in the same order, to scission events at CCSs of different sizes and lifetimes. Collectively these data reveal the fine-grained temporal structure of CME and suggest a simplified canonical model of mammalian CME in which the same core mechanism of CME, involving actin, operates at CCSs of diverse sizes and lifetimes.

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Section: Similar To Yeast Mammalian Cme Has a Modular Designmentioning

confidence: 64%

Section: Similar To Yeast Mammalian Cme Has a Modular Designmentioning

confidence: 99%

A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis

Perrais²,

2011

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“…Dynamin assembles around the neck of the flasks and squeezes it mechanically. This appears to induce a lipid-phase segregation in the membrane, which is necessary for the pinching off to take place (Liu et al, 2009;Ferguson and De Camilli, 2012). A large fraction of the clathrinindependent vesicles, however, pinch off independently of dynamin (Sandvig et al, 2011).…”

Section: Fission Of the Neck And Pinching Off Of Endocytic Vesiclesmentioning

confidence: 99%

“…A large fraction of the clathrinindependent vesicles, however, pinch off independently of dynamin (Sandvig et al, 2011). In this case, the energy could be provided by the mechanochemical feedback predicted by a model initially developed for yeast (Liu et al, 2009). Such a feedback, triggered by the curvature of the membranes, would produce an interfacial force sufficient to pinch off vesicles.…”

Section: Fission Of the Neck And Pinching Off Of Endocytic Vesiclesmentioning

confidence: 99%

Cell surface dynamics – how Rho GTPases orchestrate the interplay between the plasma membrane and the cortical cytoskeleton

Curtis

Meldolesi

2012

Journal of Cell Science

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SummarySmall GTPases are known to regulate hundreds of cell functions. In particular, Rho family GTPases are master regulators of the cytoskeleton. By regulating actin nucleation complexes, Rho GTPases control changes in cell shape, including the extension and/or retraction of surface protrusions and invaginations. Protrusion and invagination of the plasma membrane also involves the interaction between the plasma membrane and the cortical cytoskeleton. This interplay between membranes and the cytoskeleton can lead to an increase or decrease in the plasma membrane surface area and its tension as a result of the fusion (exocytosis) or internalization (endocytosis) of membranous compartments, respectively. For a long time, the cytoskeleton and plasma membrane dynamics were investigated separately. However, studies from many laboratories have now revealed that Rho GTPases, their modulation of the cytoskeleton, and membrane traffic are closely connected during the dynamic remodeling of the cell surface. Arf-and Rab-dependent exocytosis of specific vesicles contributes to the targeting of Rho GTPases and their regulatory factors to discrete sites of the plasma membrane. Rho GTPases regulate the tethering of exocytic vesicles and modulate their subsequent fusion. They also have crucial roles in the different forms of endocytosis, where they participate in the sorting of membrane domains as well as the sculpting and sealing of membrane flasks and cups. Here, we discuss how cell surface dynamics depend on the orchestration of the cytoskeleton and the plasma membrane by Rho GTPases.

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“…Initiés chez la levure, qui ne possède pas de dynamine, de nombreux travaux ont cherché à comprendre comment des modifications de la membrane plasmique pouvaient aboutir à la formation de vésicules. Il apparaît que la tension de ligne est, là encore, un élément fondamental pour expliquer la scission des vésicules [51]. Cela a été démontré d'abord in vitro grâce à un système de membranes modèles, puis in vivo dans le contexte de l'endocytose de la STx [52,53].…”

Section: Synthèse Revuesunclassified

Endocytose sans clathrine

Blouin

2013

Med Sci (Paris)

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The Mechanochemistry of Endocytosis

Abstract: An integrated theoretical model reveals how the chemical and the mechanical aspects of endocytosis are coordinated coherently in yeast cells, driving progression through the endocytic pathway and ensuring efficient vesicle scission in vivo.

Cited by 221 publications

References 56 publications

A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis

A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis

Cell surface dynamics – how Rho GTPases orchestrate the interplay between the plasma membrane and the cortical cytoskeleton

Endocytose sans clathrine

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