The mechanisms and effect of epigallocatechin gallate (EGCg) on the germination and proliferation of bacterial spores

Shigemune, Naofumi; Nakayama, Motokazu; Tsugukuni, Takashi; Hitomi, Jun; Yoshizawa, C.; Mekada, Yoko; Kurahachi, Masahiro; Miyamoto, Takahisa

doi:10.1016/j.foodcont.2012.04.003

Cited by 16 publications

(8 citation statements)

References 27 publications

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“…No changes in pH were observed when spores were incubated in defined minimal medium (buffered to pH 7.4 with 80 mM MOPS) and in the presence of the selected tea compounds (data not shown). The results presented here are in line with previous observations, which showed that polyphenols, and especially catechins, inhibit the growth of vegetative cells and not germination of spores of Bacillus species (Friedman et al, 2006;Hara-Kudo et al, 2005;Shigemune et al, 2012).…”

Section: Effect Of Different Tea Compounds On Germination Outgrowth A...supporting

confidence: 93%

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Quantitative analysis of the effect of specific tea compounds on germination and outgrowth of Bacillus subtilis spores at single cell resolution

et al. 2015

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Section: Effect Of Different Tea Compounds On Germination Outgrowth A...supporting

confidence: 93%

“…Usually, green tea contains about 30e50 mg/L of catechins, i.e. about 10% of the tea dry weight (Shigemune et al, 2012). Green tea extracts are also sold as nutraceuticals, i.e.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of the effect of specific tea compounds on germination and outgrowth of Bacillus subtilis spores at single cell resolution

et al. 2015

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“…The ability of EGCG to inhibit sporulation is well-documented across Bacillus spp. [16,57]. The findings in this study provide further evidence that tea polyphenols could serve as a potent antimicrobial agent.…”

Section: Discussionsupporting

confidence: 62%

Antibacterial and Sporicidal Activity Evaluation of Theaflavin-3,3′-digallate

Yussof

Cammalleri

Fayemiwo

et al. 2022

IJMS

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Theaflavin-3,3′-digallate (TFDG), a polyphenol derived from the leaves of Camellia sinensis, is known to have many health benefits. In this study, the antibacterial effect of TFDG against nine bacteria and the sporicidal activities on spore-forming Bacillus spp. have been investigated. Microplate assay, colony-forming unit, BacTiter-GloTM, and Live/Dead Assays showed that 250 µg/mL TFDG was able to inhibit bacterial growth up to 99.97%, while 625 µg/mL TFDG was able to inhibit up to 99.92% of the spores from germinating after a one-hour treatment. Binding analysis revealed the favorable binding affinity of two germination-associated proteins, GPR and Lgt (GerF), to TFDG, ranging from −7.6 to −10.3 kcal/mol. Semi-quantitative RT-PCR showed that TFDG treatment lowered the expression of gpr, ranging from 0.20 to 0.39 compared to the control in both Bacillus spp. The results suggest that TFDG not only inhibits the growth of vegetative cells but also prevents the germination of bacterial spores. This report indicates that TFDG is a promising broad-spectrum antibacterial and anti-spore agent against Gram-positive, Gram-negative, acid-fast bacteria, and endospores. The potential anti-germination mechanism has also been elucidated.

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“…EGCg also reduces secretion of the toxin due to EGCg-mediated enhancement of LtxA affinity for the bacterial cell surface [71]. EGCg supressed the proliferation of vegetative B. cereus and B. subtilis but not the germination of spores of these bacteria [72]. TEM indicated that EGCg was present on the surface of vegetative cells, but no deposition was detected on spore surfaces, suggesting that the compound was not adsorbed by spores.…”

Section: Non-lethal Effects On Bacterial Cellsmentioning

confidence: 99%

Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens

Taylor

2020

Molecules

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Green tea-derived galloylated catechins have weak direct antibacterial activity against both Gram-positive and Gram-negative bacterial pathogens and are able to phenotypically transform, at moderate concentrations, methicillin-resistant Staphylococcus aureus (MRSA) clonal pathogens from full β-lactam resistance (minimum inhibitory concentration 256–512 mg/L) to complete susceptibility (~1 mg/L). Reversible conversion to susceptibility follows intercalation of these compounds into the bacterial cytoplasmic membrane, eliciting dispersal of the proteins associated with continued cell wall peptidoglycan synthesis in the presence of β-lactam antibiotics. The molecules penetrate deep within the hydrophobic core of the lipid palisade to force a reconfiguration of cytoplasmic membrane architecture. The catechin gallate-induced staphylococcal phenotype is complex, reflecting perturbation of an essential bacterial organelle, and includes prevention and inhibition of biofilm formation, disruption of secretion of virulence-related proteins, dissipation of halotolerance, cell wall thickening and cell aggregation and poor separation of daughter cells during cell division. These features are associated with the reduction of capacity of potential pathogens to cause lethal, difficult-to-treat infections and could, in combination with β-lactam agents that have lost therapeutic efficacy due to the emergence of antibiotic resistance, form the basis of a new approach to the treatment of staphylococcal infections.

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The mechanisms and effect of epigallocatechin gallate (EGCg) on the germination and proliferation of bacterial spores

Cited by 16 publications

References 27 publications

Quantitative analysis of the effect of specific tea compounds on germination and outgrowth of Bacillus subtilis spores at single cell resolution

Quantitative analysis of the effect of specific tea compounds on germination and outgrowth of Bacillus subtilis spores at single cell resolution

Antibacterial and Sporicidal Activity Evaluation of Theaflavin-3,3′-digallate

Interactions of Tea-Derived Catechin Gallates with Bacterial Pathogens

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