The Mechanism of Radiation-Induced Interphase Death of Lymphoid Cells: A New Hypothesis

Eidus, L.Kh.; YuN, Korystov; Dobrovinskaja, O R; Shaposhnikova, V. V.

doi:10.2307/3577652

Cited by 15 publications

(3 citation statements)

References 21 publications

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“…Lymphocytes are one of the most radiosensitive cells (Anderson and Warner 1976;Hendry 1988;Eidus et al 1990), while their radiosensitivity varies between different subsets (Kwan and Norman 1977). This study showed that absolute concentrations of T-lymphocytes in a cohort of previously occupationally exposed Mayak workers decreased with increasing total absorbed dose from external gamma-rays, even years after the initial exposures.…”

Section: Discussionmentioning

confidence: 58%

Expression of blood serum proteins and lymphocyte differentiation clusters after chronic occupational exposure to ionizing radiation

Рыбкина

Azizova

Scherthan

et al. 2014

Radiat Environ Biophys

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This study aimed to assess effects of chronic occupational exposure on immune status in Mayak workers chronically exposed to ionizing radiation (IR). The study cohort consists of 77 workers occupationally exposed to external gamma-rays at total dose from 0.5 to 3.0 Gy (14 individuals) and workers with combined exposure (external gamma-rays at total dose range 0.7-5.1 Gy and internal alpha-radiation from incorporated plutonium with a body burden of 0.3-16.4 kBq). The control group consists of 43 age- and sex-matched individuals who never were exposed to IR, never involved in any cleanup operations following radiation accidents and never resided at contaminated areas. Enzyme-linked immunoassay and flow cytometry were used to determine the relative concentration of lymphocytes and proteins. The concentrations of T-lymphocytes, interleukin-8 and immunoglobulins G were decreased in external gamma-exposed workers relative to control. Relative concentrations of NKT-lymphocytes, concentrations of transforming growth factor-β, interferon gamma, immunoglobulins A, immunoglobulins M and matrix proteinase-9 were higher in this group as compared with control. Relative concentrations of T-lymphocytes and concentration of interleukin-8 were decreased, while both the relative and absolute concentration of natural killers, concentration of immunoglobulins A and M and matrix proteinase-9 were increased in workers with combined exposure as compared to control. An inverse linear relation was revealed between absolute concentration of T-lymphocytes, relative and absolute concentration of T-helpers cells, concentration of interferon gamma and total absorbed dose from external gamma-rays in exposed workers. For workers with incorporated plutonium, there was an inverse linear relation of absolute concentration of T-helpers as well as direct linear relation of relative concentration of NKT-lymphocytes to total absorbed red bone marrow dose from internal alpha-radiation. In all, chronic occupational IR exposure of workers induced a depletion of immune cells in peripheral blood of the individuals involved.

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Section: Discussionmentioning

confidence: 58%

Expression of blood serum proteins and lymphocyte differentiation clusters after chronic occupational exposure to ionizing radiation

Рыбкина

Azizova

Scherthan

et al. 2014

Radiat Environ Biophys

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“…In particular, the radiation-but not dexamethasone-induced apoptosis depends on p53 gene (Williams and Smith 1993). Furthermore, the membrane damage, chromatin condensation and DNA fragmentation are known to follow the different metabolic pathways (Eidus et al 1994;Schulze-Osthoff et al 1994;Sun et al 1994). Aversectin C suppresses apoptosis induced by both agents according to all criteria used.…”

Section: Discussionmentioning

confidence: 99%

“…Nuclear damage was evaluated by the chromatin condensation (nuclear pycnosis) and fragmentation of condensed chromatin (nuclear fragmentation) as described in detail elsewhere (Eidus et al 1994;SchulzeOsthoff et al 1994). Briefly the damaged nuclei were identified with acetic acid-ethanol (1:3) fixation and Giemsa staining after 6 h incubation.…”

Section: Determination Of Nuclear Damagementioning

confidence: 99%

A Comparative Study of the Effects of Aversectin C, Abamectin and Ivermectin on Apoptosis of Rat Thymocytes Induced by Radiation and Dexamethasone

Korystov¹,

Mosin²,

Shaposhnikova³

et al. 1999

Acta Vet. Brno

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The effects of avermectins on radiation-and dexamethasone-induced apoptosis of rat thymocytes have been studied. In particular, the properties of abamectin (avermectin B 1 ), ivermectin (22,23-dihydroavermectin B 1 , H 2 B 1 ) and aversectin C were examined. Aversectin C is a mixture of eight naturally occurring avermectins of the following composition: B 1 (42%), B 2 (22%), A 1 (13%) and A 2 (23%). This mixture is currently widely used as active ingredient in veterinary antiparasitic formulations produced by Pharmbiomed Co. Apoptosis was estimated by membrane damage, nuclear pycnosis (or chromatin condensation), DNA fragmentation and percentage of cells with subdiploid DNA. It was shown that aversectin C inhibits thymocyte apoptosis induced by both agents. The IC 50 dose was in the range of 0.1-0.3 µg/ml for aversectin C, whereas abamectin and ivermectin produced no effect up to 1 µg/ml. It is suggested that avermectins of the A series are responsible for the high anti-apoptotic activity exhibited by aversectin C. Thus, aversectin C is not only an effective antiparasitic drug but is also capable of exhibiting some other useful activities, e.g. a cytoprotective effect exerted on the mammalian immune cells. Avermectins, antiparasitic drugs, apoptosis, thymocytes, dexamethasone, gamma raysThe avermectins are a family of closely related 16-membered macrocyclic lactones produced by the fungus Streptomyces avermitilis. Nearly all the avermectins exhibit a broad spectrum of activity against nematode and arthropode parasites, with the B 1a compound being the most potent (Campbell 1989). Accordingly, it was used as active ingredient in commercial antiparasitic formulations. Until recently, there were basically two types of such avermectin-based active ingredients, i.e. ivermectin (consisting mainly of avermectin H 2 B 1a ) and abamectin (predominantly containing avermectin B 1a ). However, several years ago the Pharmbiomed Co. started manufacturing the veterinary and plant protection formulations based on a new active ingredient -aversectin C which is an intrinsic mixture of eight naturally occurring avermectins. Preliminary experiments demonstrated that aversectin C (presumably due to the presence of avermectins of both the A-and B-series) possesses some new useful features compared to ivermectin and abamectin, e.g. with the same high efficacy, the spectrum of activity is broader, level of residues is lower and, hence, the shorter withdrawal periods could be recommended (Simetsky et al. 1994;Berezkina et al. 1996). Furthermore, degradation of aversectin C in soil and water was found to be more effective compared to abamectin and ivermectin ( M o sin et al. 1998) and, hence, ecologically aversectin C is safer.

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Cell Loss in Irradiated Tumours

Brammer

Jung

1993

Medical Radiology

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The Mechanism of Radiation-Induced Interphase Death of Lymphoid Cells: A New Hypothesis

Cited by 15 publications

References 21 publications

Expression of blood serum proteins and lymphocyte differentiation clusters after chronic occupational exposure to ionizing radiation

Expression of blood serum proteins and lymphocyte differentiation clusters after chronic occupational exposure to ionizing radiation

A Comparative Study of the Effects of Aversectin C, Abamectin and Ivermectin on Apoptosis of Rat Thymocytes Induced by Radiation and Dexamethasone

Cell Loss in Irradiated Tumours

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