The mechanism of polysaccharide synthesis of Sanghuangporus sanghuang based on multi-omic analyses and feedback inhibition

Li, Tingting; Wang, Qin; Yang, Yan; Song, Dingka

doi:10.1016/j.carbpol.2023.121288

Cited by 6 publications

(2 citation statements)

References 37 publications

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“…The Sanghuangporus exopolysaccharide is an extensively studied active product obtained from the Sanghuangporus spp. extract [6][7][8]. It is one of the important active ingredients of Sanghuangporus alpinus [9].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Fermentation Culture Medium for Sanghuangporus alpinus Using Response-Surface Methodology

Gao,

Li,

et al. 2024

Horticulturae

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The newly identified Sanghuangporus alpinus species of the Sanghuang mushroom genus has been found to possess significant medical benefits. However, the current artificial cultivation technology has not reached the requisite maturity. The response-surface methodology (RSM) was used to optimize the Sanghuangporus alpinus culture medium formulation and evaluate the functional activity of S. alpinus exopolysaccharides. First, a single-factor experiment was conducted to screen for optimal carbon and nitrogen sources for S. alpinus. Then, using Box–Behnken’s central composite design, a response-surface experiment was conducted to determine optimal culture parameters. Finally, the rationality of those parameters was assessed in a shaking flask experiment. The optimal culture parameters, determined through regression analysis, were 20.20 ± 0.17 g/L fructose (carbon source), 7.29 ± 0.10 g/L yeast extract (nitrogen source), and 0.99 ± 0.01 g/L dandelion. With optimization, the S. alpinus yield increased to 12.79 ± 1.41 g/L, twice that obtained from the initial culture medium. The S. alpinus exopolysaccharide exhibited an excellent antioxidant capacity, with the strongest scavenging effect noted on ABTS free radicals (lowest half-inhibitory concentration: 0.039 mg/mL). Additionally, this exopolysaccharide effectively inhibited various cancer cells, exhibiting the strongest activity against human glioma cells U251 (half-inhibitory concentration: 0.91 mg/mL). The RSM used to optimize the fermentation culture parameters of S. alpinus significantly increased the mycelial biomass. The improvement of Sanghuangporus alpinus yield through liquid fermentation and optimizing the fermentation medium could fill the existing gap in the cultivation of Sanghuangporus alpinus, as well as provide valuable data for the large-scale production of S. alpinus.

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Section: Introductionmentioning

confidence: 99%

Optimization of Fermentation Culture Medium for Sanghuangporus alpinus Using Response-Surface Methodology

Gao,

Li,

et al. 2024

Horticulturae

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“…polysaccharide is an important active substance of Sanghuangporus spp. Intracellular polysaccharides are mainly derived from fermentation broth [6][7][8][9]. The extracellular polysaccharides of S. sanghuang are widely-studied as bioactive compounds and show a broad range of pharmacological activities, including anticancer [10], hypoglycemic [11], antioxidant [12], and immunity-enhancing [13] properties.…”

Section: Introductionmentioning

confidence: 99%

The Liquid-Fermentation Formulation of Sanghuangporus sanghuang Optimized by Response Surface Methodology and Evaluation of Biological Activity of Extracellular Polysaccharides

Gao,

Li,

et al. 2024

Foods

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Sanghuangporus sanghuang is a rare fungus growing on mulberry trees that has immense medicinal value. This study aimed to optimize the liquid-fermentation-media formulation and culture conditions for large-scale culturing of S. sanghuang by performing one-way testing and response surface methodology. The antioxidant and anticancer activities of the extracellular polysaccharides from S. sanghuang were also analyzed. The optimal formulation and growth conditions for S. sanghuang were as follows: glucose, 30.2 ± 0.37 g/L; yeast extract, 14.60 ± 0.05 g/L; dandelion powder, 1.24 ± 0.01 g/L; shaker speed, 150 r/min; and temperature, 25 °C. We obtained 13.99 ± 0.42 g/L of mycelium biomass by culturing S. sanghuang for 15 days with the optimized formulation. This was 2-fold higher than the mycelial mass obtained with the sub-optimal formulation. The extracellular fungal polysaccharides showed significant antioxidant activity against ABTS and DPPH free radicals, and significantly reduced the in vitro growth and survival of several cancer cell lines. The anticancer activity of the extracellular fungal polysaccharides was significantly higher in the human glioma cells than in other cancer cell lines. In summary, this study optimized the liquid media formulation and conditions for the large-scale culturing of S. sanghuang. Furthermore, the extracellular polysaccharides from S. sanghuang showed significant antioxidant and anticancer activities.

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Polypore funga and species diversity in tropical forest ecosystems of Africa, America and Asia, and a comparison with temperate and boreal regions of the Northern Hemisphere

Zhao,

Wu,

Yang

et al. 2024

Forest Ecosystems

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The mechanism of polysaccharide synthesis of Sanghuangporus sanghuang based on multi-omic analyses and feedback inhibition

Cited by 6 publications

References 37 publications

Optimization of Fermentation Culture Medium for Sanghuangporus alpinus Using Response-Surface Methodology

Optimization of Fermentation Culture Medium for Sanghuangporus alpinus Using Response-Surface Methodology

The Liquid-Fermentation Formulation of Sanghuangporus sanghuang Optimized by Response Surface Methodology and Evaluation of Biological Activity of Extracellular Polysaccharides

Polypore funga and species diversity in tropical forest ecosystems of Africa, America and Asia, and a comparison with temperate and boreal regions of the Northern Hemisphere

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