The Mechanism of Hemagglutination by Influenza Virus

Buzzell, Anne; Hanig, Martin

doi:10.1016/s0065-3527(08)60676-4

Cited by 19 publications

(5 citation statements)

References 174 publications

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“…In contrast to the findings with enteroviruses, myxovirus hemagglutinating activity was not inactivated by sulfhydryl reagents. The myxoviruses attach to mucoprotein receptors on erythrocytes (2) from which N-acetylneuraminic acid is split by an enzyme, neuraminidase (29). The virus particle possesses this enzyme, and it is also produced by the bacterium Vibrio cholerae (30,31).…”

Section: Discussionmentioning

confidence: 99%

On the Role of Virus Sulfhydryl Groups in the Attachment of Enteroviruses to Erythrocytes

Philipson¹,

Choppin²

1960

The Journal of Experimental Medicine

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Many animal viruses possess the ability to agglutinate erythrocytes. In most but not all cases hemagglutination is due to the virus particle itself, and appears to result from the mechanical bridging of two or more erythrocytes by virus particles which attach to receptor sites on each erythrocyte (1, 2). Thus, attachment of virus particles to erythrocytes is a prerequisite for hemagglutination, and prevention of absorption of virus prevents hemagglutination. Among the enteroviruses, many ECHO viruses and some strains of Coxsackie B3 virus agglutinate human erythrocytes (3-7), and the evidence indicates that hemagglutination is due to the virus particle itself (3, 5, 6). The precise mechanism of attachment of enteroviruses to cells is unknown. Chymotrypsin treatment of erythrocytes prevents the absorption of some ECHO viruses (8). This suggests that the receptor sites on the erythrocyte may be at least in part protein in nature. The present communication is concerned with the mechanism of attachment of enteroviruses to cells. It is shown that sulfhydryl reagents block the hemagglutinating activity of enteroviruses. Treated virus fails to absorb to erythrocytes. Thiol compounds restore the hemagglutinating activity of enteroviruses treated with mercaptide-forming sulfhydryl reagents. The effect of sulfhydryl reagents on the infectivity of some enteroviruses is also described.

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Section: Discussionmentioning

confidence: 99%

On the Role of Virus Sulfhydryl Groups in the Attachment of Enteroviruses to Erythrocytes

Philipson¹,

Choppin²

1960

The Journal of Experimental Medicine

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“…Prior to testing the retrospective samples, the relative sensitivities of the MGB Alert, ProFlu-1 RT-PCR, and the TMA assays were tested using influenza RNA in spiked samples. Nucleic acid amplification is more difficult using a whole blood sample; however, there are theoretical reasons to suspect that the virus might preferentially compartmentalize in the red blood cell fraction of blood through interaction of hemagglutinin with the surface of red blood cells and platelets [30,31]. Therefore, in an attempt to maximize assay sensitivity, influenza-spiked blood aliquots were separated into the plasma fraction for testing or tested as a whole blood aliquot.…”

Section: Relative Sensitivity Of Influenza Rna Detection Assaysmentioning

confidence: 99%

Sensitive Detection Assays for Influenza RNA Do Not Reveal Viremia in US Blood Donors

Stramer

Collins²,

Nugent³

et al. 2012

The Journal of Infectious Diseases

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“…Thrombocytopenia in the setting of influenza infection has been noted in a number of case reports, often in the setting of concomitant antibiotic therapy, which can also affect the platelet (PLT) count 50,52,53 . Although influenza does not appear to cause major hematologic perturbations in patients, it has been shown to interact with blood elements 54 . Influenza has been shown to adsorb to PLTs and RBC with equal kinetics and to elute from PLTs more slowly and less completely than from RBCs 55 .…”

Section: Evidence For Influenza Viremia In Human Infectionmentioning

confidence: 99%