The Mechanism of Cold-Induced Platelet Aggregation in the Presence of Heparin.

Akiyama, Masaki; Takami, Hideki; Yoshida, Yutaka

doi:10.1620/tjem.177.365

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…Although in the present study we did not investigate the mechanism of monocyte-platelet association, we consider that it may result from gradual platelet and monocyte activation. Blood cooling has been shown to activate platelets and promote platelet aggregation 28 and, indeed, we observed some platelet-bearing monocytes after blood storage on ice. Stimulation of monocytes by subsequent incubation of blood with LPS at 37°C leads to further monocyte-platelet association.…”

Section: Discussionsupporting

confidence: 66%

Monocyte Integrin Expression And Monocyte–Platelet Complex Formation In Humans With Coronary Restenosis

Arefieva

Проваторов

Самко

et al. 2001

Clin Exp Pharma Physio

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1. In the present study, we sought to determine whether patients with restenosis after coronary stenting possess increased monocyte reactivity, as manifested by a higher level of adhesion molecule expression and an enhanced propensity to form monocyte-platelet aggregates after activation in vitro. 2. Anti-coagulated peripheral venous blood from 24 patients, 10 with and 14 without angiographically verified restenosis, was obtained. Leucocyte antigen expression and the number of leucocyte-platelet complexes were measured by flow cytometry after activation in whole blood. 3. Surface integrin Mac-1 (CD11b/CD18) and VLA-4 (CD49d/ CD29) expression on monocytes and the relative number of monocyte-platelet complexes after in vitro activation were significantly elevated in patients with restenosis compared with patients without restenosis (fluorescence intensities of 1425 +/- 76 vs 1195 +/- 71, 87 +/- 7 vs 65 +/- 6 and 47 +/- 4 vs 29 +/- 3% for for Mac-1, VLA-4 and monocyte-platelet complexes, respectively; P < 0.05 for each parameter). 4. The results suggest that restenosis is associated with increased monocyte VLA-4 and Mac-1 integrin expression and monocyte-platelet complex formation, which can be revealed after activation in vitro.

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Section: Discussionsupporting

confidence: 66%

Monocyte Integrin Expression And Monocyte–Platelet Complex Formation In Humans With Coronary Restenosis

Arefieva

Проваторов

Самко

et al. 2001

Clin Exp Pharma Physio

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“…These results suggest that not only renal function improvement may decrease the uremia-induced cardiovascular injury, lowering inflammation and oxidative stress, but that, in turn, the recipient pre-transplant cardiovascular and/or metabolic status may profoundly impact graft vascularization and function at follow-up. The use of serum rather than plasma may have determined the artificial generation of platelet-derived EVs; however, low-speed centrifugation may determine in vitro cold-induced platelet activation also in plasma samples [ 27 , 28 ]. EV release by platelets in this circumstance is not fully standardizable, thus making EV quantitative data less reliable.…”

Section: Discussionmentioning

confidence: 99%

Identification of a serum and urine extracellular vesicle signature predicting renal outcome after kidney transplant

Burrello

Monticone

Burrello

et al. 2022

Nephrology Dialysis Transplantation

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Background A long-standing effort is dedicated toward identification of biomarkers allowing the prediction of graft outcome after kidney transplant. Extracellular vesicles (EVs) circulating in body fluids represent an attractive candidate, as their cargo mirrors the originating cell and its pathophysiological status. The aim of the study was to investigate EV surface antigens as potential predictors of renal outcome after kidney transplant. Methods We characterized 37 surface-antigens by flow-cytometry, in serum- and urine- EVs from 58 patients which were evaluated before, and at 10-14 days, 3 months, and 1 year after transplant, for a total of 426 analyzed samples. The outcome was defined according to estimated glomerular filtration rate (eGFR) at 1 year. Results Endothelial cells and platelets markers (CD31, CD41b, CD42a and CD62P) in serum-EVs were higher at baseline in patients with persistent kidney dysfunction at 1 year, and progressively decreased after kidney transplant. Conversely, mesenchymal progenitor cell marker (CD1c, CD105, CD133, SSEEA-4) in urine-EVs progressively increased after transplant in patients displaying renal recovery at follow-up. These markers correlated with eGFR, creatinine and proteinuria, associated to patient outcome at univariate analysis and were able to predict patient outcome at ROC curves analysis. A specific EV molecular signature obtained by supervised learning correctly classified patients according to 1-year renal outcome. Conclusions An EV-based signature, reflecting the cardiovascular profile of the recipient, and the repairing/regenerative features of the graft, could be introduced as a non-invasive tool for a tailored management of follow-up of patients undergoing kidney transplant.

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“…[17,35] Similarly, platelets undergo activation-like effects when exposed to cold, which can result in platelet adhesion to the endothelium of the capillaries where tissue factor is exposed during endothelial trauma. [10,[36][37][38] Finally, leukocytes can be activated by both cold and blood stasis, which increases cell-cell interactions, especially with the sinusoidal endothelium, leading to in ammation and cellular adhesion. [39] This work aimed to see if these known effects of cold on blood translated to the clinical context of retained peripheral blood after ushing a liver allograft during procurement.…”

Section: Discussionmentioning

confidence: 99%

The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

Haque

Pendexter

Wilks

et al. 2021

Preprint

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In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 hours of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush.

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The Mechanism of Cold-Induced Platelet Aggregation in the Presence of Heparin.

Cited by 8 publications

References 17 publications

Monocyte Integrin Expression And Monocyte–Platelet Complex Formation In Humans With Coronary Restenosis

Monocyte Integrin Expression And Monocyte–Platelet Complex Formation In Humans With Coronary Restenosis

Identification of a serum and urine extracellular vesicle signature predicting renal outcome after kidney transplant

The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

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