The Mechanism of Cleavage in Animal Cells

Swann, M. M.; Mitchison, J.M.

doi:10.1111/j.1469-185x.1958.tb01409.x

Cited by 104 publications

(43 citation statements)

References 60 publications

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“…A possible relationship between cell locomotion and cytokinesis has been suggested previously (for review, Swann and Mitchison, 1958;Taylor and Fechheimer, 1982;Bray and White, 1988;Janson and Taylor, 1993). Final cell separation at the conclusion of cytokinesis yields two daughter cells that exhibit patterns of rearward transport of transverse fibers, the contraction of the transverse fibers as they transport toward the nucleus, and the gradient of myosin II RLC phosphorylation, low at the leading edge and increasing posteriorly toward (Figures 6 and 9).…”

Section: Discussionmentioning

confidence: 99%

“…century (for review, Swann and Mitchison, 1958;Rap-Investigating the mechanism of cytokinesis has been a paport, 1986; Salmon, 1989;Mabuchi and Itoh, 1992; major target of cell biological research for more than a Satterwhite and Pollard, 1992;Fishkind and Wang, 1995 (Sanger, 1975;Maupin and Pollard, 1986;Fishkind and Wang, 1993;Mabuchi, 1994). A combination of biochemical, genetic, molecular, biophysical, and structural approaches, applied to a range of organisms, are beginning to reveal important details of the steps, components, and mechanics involved in cytokinesis (Sanders and Field, 1994;Fishkind and Wang, 1995;Miller and Kiehart, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.

DeBiasio

LaRocca

Post

et al. 1996

MBoC

136

104

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The mechanism of cytokinesis has been difficult to define because of the short duration and the temporal-spatial dynamics involved in the formation, activation, force production, and disappearance of the cleavage furrow. We have investigated the structural and chemical dynamics of myosin II in living Swiss 3T3 cells from prometaphase through the separation and migration of daughter cells. The structural and chemical dynamics of myosin II have been defined using the semiautomated, multimode light microscope, together with a fluorescent analogue of myosin II and a fluorescent biosensor of myosin II regulatory light chain (RLC) phosphorylation at serine 19. The correlation of image data from live cells using different modes of light microscopy allowed interpretations not possible from single-mode investigations. Myosin II transported toward the equatorial plane from adjacent regions, forming three-dimensional fibers that spanned the volume of the equator during anaphase and telophase. A global phosphorylation of myosin II at serine 19 of the RLC was initiated at anaphase when cortical myosin II transport started. The phosphorylation of myosin II remained high near the equatorial plane through telophase and into cytokinesis, whereas the phosphorylation of myosin II at serine 19 of the RLC decreased at the poles. The timing and pattern of phosphorylation was the same as the shortening of myosin II-based fibers in the cleavage furrow. Myosin II-based fibers shortened and transported out of the cleavage furrow into the tails of the two daughter cells late in cytokinesis. The patterns of myosin II transport, phosphorylation, and shortening of fibers in the migrating daughter cells were similar to that previously defined for cells migrating in a wound in vitro. The temporal-spatial patterns and dynamics of myosin II transport, phosphorylation at serine 19 of the RLC, and the shortening and disappearance of myosin II-based fibers support the proposal that a combination of the cortical flow hypothesis and the solation-contraction coupling hypothesis explain key aspects of cytokinesis and polarized cell locomotion.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.

DeBiasio

LaRocca

Post

et al. 1996

MBoC

136

104

View full text Add to dashboard Cite

show abstract

“…Since each aster has the same effect, it has been suggested that the necessity for two is based upon a requirement for additive astral activity [20,21], or, alternatively, upon a requirement that both polar surfaces simultaneously interact with the asters [11,15,[22][23][24].…”

Section: Discussionmentioning

confidence: 99%

“…Two hypothetical patterns are considered possible at present. According to the polar stimulation hypothesis [11,15], the polar surfaces are altered by the asters to a greater degree than the equatorial surface because the astral centers are closer to the polar surfaces and the intensity of interaction is inversely proportional to the distance between the interactants. According to the equatorial stimulation hypothesis, it is the equatorial surface that is altered by the asters, because they act upon it in some way that is additive.…”

Section: Hypothetical Stimulus Patternsmentioning

confidence: 99%

Mitotic apparatus-surface interaction and cell division

Rappaport

1986

International Journal of Invertebrate Reproduction and Developm

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“…According to his interpretation, the RNP, which the chromosome acquired at prophase, bubbles as it is shed from the chromosomes at anaphase. Swann and Mitchison (1958), observing sea urchin eggs during cleavage, suggested that bubbling may be caused by the expansion of the cell surface resulting from the release of certain substances from chromosomes during anaphase. Ethylenediaminetetraacetic acid (EDTA) and victoria blue elicit similar bubbling in fibroblasts (Dornfeld and Owczarzak, 1958;Lettr6 and Schleich, 1955).…”

Section: Discussionmentioning

confidence: 99%