The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines

Bae, Chang Jun; Park, S E; Seong, Young-Joon; Kimm, Seung-Won; Park, J B

doi:10.3346/jkms.1993.8.2.153

Cited by 6 publications

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“…As shown in Figure 2, synthesis rate of erb B-2 protein in SNU-1 cells was increased compared to SNU-16 cells while the half-life of erb B-2 protein was not significantly different ( Figure 1). This enhanced protein synthesis rate was also observed in SNU-5 cells, showing erb B-2 protein overexpression without mRNA increase (Bae et al, 1993;1996).…”

Section: Discussionmentioning

confidence: 62%

“…In addition to overexpression of erb B-2 at mRNA level, several reports suggested that post-transcriptional upregulation of c-erb B-2 might exist (Burhing et al, 1995;Oshima et al, 1995;Child et al, 1999). We earlier reported post-transcriptional overexpression of erb B-2 (Bae et al, 1993). In that study, erb B-2 protein level was upregulated without mRNA increase in SNU-1 and SNU-5 stomach cancer cells compared to SNU-16 or KATO III cells.…”

Section: Introductionmentioning

confidence: 65%

“…Earlier report showed that expression level of erb B-2 protein in SNU-1 and SNU-5 cells 2 to 4 times higher than SNU-16 or KATO III cells without gene amplification or mRNA overexpression (Bae et al, 1993). Moreover, this overexpression of erb B-2 was gene-specific in that overall protein synthesis rate in SNU-1 or SNU-16 cells was similar.…”

Section: Half Life Of Erb B-2 Protein In Snu-1 Cellsmentioning

confidence: 78%

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Post-transcriptional control of c-erb B-2 overexpression in stomach cancer cells

Bae

Juhnn²,

Park³

2001

Exp Mol Med

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The growth factor receptor oncogene, c-erb B-2, is frequently overexpressed in the adenocarcinomas of breast, ovary, lung and stomach. Although the mechanism of erb B-2 overexpression is thought as the result of transcriptional upregulation in many primary human carcinomas, expression rate of c-erb B-2 at mRNA level is usually lower than the level of translated protein. We also found that the expression of erb B-2 in SNU-1 stomach cancer cells was greater at post-transcription level (Bae et al., 1993). To explore the underlying mechanism of erb B-2 protein overexpression, we have chosen two cells lines, SNU-1 and SNU-16 where transcription rate of erb B-2 was closely resemble to each other while expressed protein levels were quite different. The synthesis rate of erb B-2 protein in SNU-1 cells was faster than SNU-16 cells while levels of erb B-2 mRNA were found to be similar in both cell lines. The half-life of the expressed erb B-2 protein was not significantly different in both cell lines. Analysis of the 5' untranslated region (UTR) of erb B-2 mRNA (-1~-323) showed no sequence abnormality in both cell lines. However, ribonuclease protection assay using cloned 5 UTR sequence revealed that the size of 5' UTR of erb B-2 mRNA which associate with transcription initiation site(s) in SNU-1 cells was longer than that in SNU-16. These results suggest that the increased erb B-2 protein synthesis rate possibly due to the redundant selection of transcription initiation might be a mechanism of erb B-2 overexpression in SNU-1 cells.

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 65%

Section: Half Life Of Erb B-2 Protein In Snu-1 Cellsmentioning

confidence: 78%

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Post-transcriptional control of c-erb B-2 overexpression in stomach cancer cells

Bae

Juhnn²,

Park³

2001

Exp Mol Med

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“…4). The variant 4b was highly expressed in most of metastasis‐related gastric cancer cell lines, which include KATO III, MKN74, and Hs746T (derived from metastatic site), SNU1 and SNU16 (derived from poorly differentiated tumors 27 with overexpression of ERBB2/HER2/neu 28, 29), and the highly metastatic cell line MKN45P 45. Similar high expression of variant 4b was observed in stomach tumor tissue (derived from metastatic adenocarcinoma).…”

Section: Discussionmentioning

confidence: 66%

“…KATO III, MKN74, and Hs746T were derived from metastatic sites. SNU1 and SNU16 were established from poorly differentiated tumors 27 and shown overexpression of ERBB2 ( HER2/neu ) that have been related to metastatic ability 28, 29. Stomach tumor tissue was derived from adenocarcinoma isolated from 77‐year‐old female Caucasian, the metastases of which were seen in lymph nodes.…”

Section: Resultsmentioning

confidence: 99%

Detection of a Pathway From Linoleate to a Novel Cyclopentenone: cis‐12‐Oxo‐10‐Phytoenoic Acid in Sunflower Roots

et al. 2007

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The lipoxygenase pathway in sunflower roots was studied in vitro. A preliminary incubation of linoleic acid with 15 000 g supernatant of homogenate of sunflower roots (1.5-6 days after germination) revealed the predominant activity of 13-lipoxygenase. The exogenously added linoleic acid 13-hydroperoxide is further utilized through two competing pathways. One of them is directed towards formation of the ketodiene (9Z,11E)-13-oxooctadeca-9,11-dienoic acid. The second pathway, which is controlled by allene oxide synthase, leads to the formation of an alpha-ketol and a novel cyclopentenone, rac-cis-12-oxo-10-phytoenoic acid (12-oxo-PEA) via a short-lived allene oxide. Unexpectedly, the cyclopentenone 12-oxo-PEA is the predominant allene oxide synthase product. Identification of cis-12-oxo-PEA was confirmed by its UV, mass, (1)H NMR and 2D-COSY spectral data. The highest yield of 12-oxo-PEA is observed in very young roots (1.5-2 days after germination). The results of methanol-trapping experiments demonstrate that both 12-oxo-PEA and alpha-ketol are formed through the unstable allene oxide intermediate, (9Z)-12,13-epoxyoctadeca-9,11-dienoic acid, which is the primary product of allene oxide synthase. Since 12-oxo-PEA is a jasmonate congener, its biosynthesis in plants might be of physiological importance.

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C-erb-B2 (HER2/neu) expression in synovial sarcoma of the head and neck

et al. 2005

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These results demonstrate that C-erb-B2 (HER2/neu) may play a role in the tumorigenesis of synovial sarcoma; and, therefore, antigrowth factor therapies may provide a previously unrecognized pharmaceutical approach to soft tissue tumors. The data also suggest that although synovial sarcoma of the head and neck and synovial sarcoma of the extremities have similar morphologic features, they may be clinically and mechanistically distinct entities.

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The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines

Cited by 6 publications

References 0 publications

Post-transcriptional control of c-erb B-2 overexpression in stomach cancer cells

Post-transcriptional control of c-erb B-2 overexpression in stomach cancer cells

Detection of a Pathway From Linoleate to a Novel Cyclopentenone: cis‐12‐Oxo‐10‐Phytoenoic Acid in Sunflower Roots

C-erb-B2 (HER2/neu) expression in synovial sarcoma of the head and neck

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