The Mechanism of Antifungal Action of a New Polyene Macrolide Antibiotic Antifungalmycin 702 from Streptomyces padanus JAU4234 on the Rice Sheath Blight Pathogen Rhizoctonia solani

Xiong, Zhiqiang; Tu, Xiao-Rong; Wei, Sai-Jin; Huang, Lin; Li, Xunhang; Lü, Hui; Guo-quan, TU

doi:10.1371/journal.pone.0073884

Cited by 22 publications

(15 citation statements)

References 14 publications

(27 reference statements)

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“…Biocontrols of rice sheath blight by antagonistic microorganisms including Bacillus species (Kakar et al, 2018;Peng et al, 2014;Shrestha et al, 2016), Streptomyces species inclusive of Streptomyces aurantiogriseus VSMGT1014, Streptomyces philanthi RM-1-138, and Streptomyces padanus JAU4234 (Boukaew and Prasertsan, 2014;Harikrishnan et al, 2014;Xiong et al, 2013) and Trichoderma species (Chen et al, 2015) have been reported. Recent study indicated that antifungalmycin N2 produced by Streptomyces sp.…”

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confidence: 99%

Field Sanitation and Foliar Application of Streptomyces padanus PMS-702 for the Control of Rice Sheath Blight

Yang

Huang

2021

Plant Pathol J

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Rice sheath blight (ShB), caused by Rhizoctonia solani Kühn AG1-IA, is one of the destructive rice diseases worldwide. The aims of this study were to develop biocontrol strategies focusing on field sanitation and foliar application with a biocontrol agent for ShB management. Streptomyces padanus PMS-702 showed a great antagonistic activity against R. solani. Fungichromin produced by S. padanus PMS-702, at 3.07 mg/l inhibited 50% mycelial growth, caused leakage of cytoplasm, and inhibited the formation of infection structures of R. solani. Fungichromin could reach to 802 mg/l when S. padanus PMS-702 was cultured in MACC broth for 6 days. Addition of 0.5% S. padanus PMS-702 broth into soil decreased the survival rate of the pathogen compared to the control. Soil amended with 0.5% S. padanus broth and 0.5% tea seed pomace resulted in the death of R. solani mycelia in the infested rice straws, and the germination of sclerotia was inhibited 21 days after treatment. Greenhouse trials revealed that S. padanus cultured in soybean meal-glucose (SMGC-2) medium after mixing with different surfactants could enhance its efficacy for inhibiting the pathogen. Of six surfactants tested, the addition of 2% tea saponin was the most effective in suppressing the pathogen. S. padanus broth after being fermented in SMGC-2, mixed with 2% tea saponin, diluted 100 fold, and sprayed onto rice plants significantly reduced ShB disease severity. Thus, S. padanus PMS-702 is an effective biocontrol agent. The efficacy of S. padanus PMS-702 for disease control could be improved through formulation.

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confidence: 99%

Field Sanitation and Foliar Application of Streptomyces padanus PMS-702 for the Control of Rice Sheath Blight

Yang

Huang

2021

Plant Pathol J

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“…From ( Table 1, Molecular factors responsible for the resistance phenotype in the cell identified has been reported to include over expression of two types of efflux pumps, mutation of the target enzyme, and alteration of other enzymes in the same biosynthetic pathway as the target enzyme (53,11). Generally, azoles and polyenes actions are mainly directed against ergosterol, the major sterol of the fungal plasma membrane, which is analogous to cholesterol in mammalian cells, (13)(14). The dosing schedule may have an effect on the development of resistance (4), since patients treated with intermittent therapy were more likely to develop resistance than those treated continuously.…”

Section: Resultsmentioning

confidence: 99%

“…The depicted different levels of exposure to various antifungal agents tested may be a pointer to emergence of resistant clones of oropharyngeal yeast strains among the HIV infected patients studied. The frequency of diagnosed fungal infections is known to have risen due to increase in the number of immunosuppressed patients resulting from the AIDS epidemic and increase in the frequency of recalcitrant infections to standard antifungal therapy (13)(14), Susceptibility of isolates to antifungals varied and MIC values were significantly (p<0.05) dependent on district of sample collection. Thus, despite being treated with same guidelines by clinicians in the same TASO centres, patient's uptake and compliance to treatment guidelines were different, thus depicting different levels of exposure to antifungals agents and probable prescription pattern.…”

Section: Resultsmentioning

confidence: 99%

“…Polyenes and Azoles remain the antifungal agents most commonly used to treat Candida infections in the immunocompromised patients living in Uganda. The polyenes like amphotericin B and nystatin are fungicidal agents that bind preferentially to the ergosterol found in fungal membranes (12)(13). This disrupts the integrity of the cellular membrane, causing cell death.…”

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confidence: 99%

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Idiosyncratic response of yeasts, recovered from oropharyngeal lesions of HIV patients, to prophylactic antifungal agents, in South Western Uganda

Agwu¹

2019

Preprint

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Background: Although cutting edge technologies have advanced overall knowledge of microbial infections, what microorganisms will do when challenged with surface active agents is incompletely understood and understanding the characteristic uniqueness of an organism when challenged with antimicrobial agents derails antibiotic stewardship programs in resource limited settings. T his study was therefore designed to determine the idiosyncratic in-vitro response of yeast strains, challenged with selected routine antifungal agents used for routine prophylactic management of HIV infected Methods: A total of 304 (235 females and 69 males) patients with different conditions were recruited. The analysis performed included: cultivation on Sabouraud Dextrose Agar, Corn Meal Agar, Potato Dextrose Agar, germ-tube test, chlamydospore production, test for ability to grow at 45 o C. For further morphologic identification, yeasts were grown on CHROMagar Candida medium at 37 + 2 0 C for 24-48 hours and sub-cultured on Pal’s agar. The minimum inhibitory concentration (MIC) of the isolates were determined by the E-test stable agar gradient method (AB BIODISK, Solna, Sweden) Results: The 304 (235 females and 69 males) recruited patients with different oral lesions consented to this study and 304 sample surveyed was guided by the upper limit required to give 95% level of confidence at an expected prevalence of about 74% (Agwu et al, 2008) using : Sample size (N) = Z 2 P (100-P)/D2 giving 296 samples, (Epi-info version 3.2 data-base; 1995), where Z is a constant given as (1.96), P is expected prevalence (74%), and D is acceptable error (5%). Informed consent was sought and obtained from the following: Uganda National Council of Science and Technology, Kampala International University Research and Ethics Committee, HIV patients of The AIDS Support Organizations,. Conclusion : Candida albicans remain significantly susceptible to tested antifungal agents. Emerging dose dependent susceptibility in non albicans oropharyngeal yeast strains is interesting in this era of increasing treatment failures due to drug resistance. Pattern of resistance recorded may suggest significant contribution of yeast in the recurrent or delayed oropharyngeal lesions of HIV population studied. Sustained surveillance with MIC which impacts treatment outcome is recommended

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“…Using chemical fungicide can effectively control the severity of R. solani, however, chemical fungicide is generally considered to be among the potential health and environmental risks . Hence, to reduce the harmful effects to the environmental search for novel natural product-based fungicide, has gained attention in the past decades (Xiong et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The Inhibitory Effect of the Plant Alkaloid Camptothecin on the Rice Sheath Blight Pathogen Rhizoctonia solani

Dai¹,

Xu²,

Zhou³

et al. 2017

IJAB

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The rice sheath blight caused by Rhizoctonia solani is one of the most destructive diseases of rice worldwide. Present study presented the inhibitory effect and mechanism of the plant alkaloid camptothecin on R. solani. Results indicated 14.32, 34.88, 59.94, 68.54, 76.38 and 77.72% inhibition of mycelial growth after 48 h of incubation at 6.25, 12.50, 25.00, 50.00, 100 and 200 μg/mL camptothecin. The soluble protein content in mycelia was significantly increased, the N-acetyl glucosamine content and chitinase activity was significantly reduced, but the sugar content was unaffected by camptothecin at 100 μg/mL after 12 h and 24 h treatment. Moreover, the optical microscopic and transmission electron microscopic observation revealed that the fungal mycelia are sparse with a loose structure, mitochondrial nule3mber, and morphological characteristics of camptothecin-treated R. solani. Overall, the antifungal activity of camptothecin against R. solani, suggested that camptothecin has a great potential in the control of rice sheath blight.

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The Mechanism of Antifungal Action of a New Polyene Macrolide Antibiotic Antifungalmycin 702 from Streptomyces padanus JAU4234 on the Rice Sheath Blight Pathogen Rhizoctonia solani

Cited by 22 publications

References 14 publications

Field Sanitation and Foliar Application of Streptomyces padanus PMS-702 for the Control of Rice Sheath Blight

Field Sanitation and Foliar Application of Streptomyces padanus PMS-702 for the Control of Rice Sheath Blight

Idiosyncratic response of yeasts, recovered from oropharyngeal lesions of HIV patients, to prophylactic antifungal agents, in South Western Uganda

The Inhibitory Effect of the Plant Alkaloid Camptothecin on the Rice Sheath Blight Pathogen Rhizoctonia solani

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