The Mechanism of Allantoin Degradation by a Pseudomonas

Campbell, L. Leon

doi:10.1128/jb.68.5.598-603.1954

Cited by 40 publications

(8 citation statements)

References 20 publications

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“…This report was based on the formation of labelled formate and carbon dioxide when the extracts were shaken for 6hr. with 2mM-hypophosphite, which inhibits the oxidation of formate (Campbell, 1954). The mechanism of the reaction may be the same as that suggested by Wood (1964) for the metabolism of glyoxylate by extracts of a Pseudomona sp.…”

Section: Discussionsupporting

confidence: 63%

The metabolism of glyoxylate by cell-free extracts of Pseudomonas sp

Bailey

Hullin

1966

Biochemical Journal

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1. Extracts of Pseudomonas sp. grown on butane-2,3-diol oxidized glyoxylate to carbon dioxide, some of the glyoxylate being reduced to glycollate in the process. The oxidation of malate and isocitrate, but not the oxidation of pyruvate, can be coupled to the reduction of glyoxylate to glycollate by the extracts. 2. Extracts of cells grown on butane-2,3-diol decarboxylated oxaloacetate to pyruvate, which was then converted aerobically or anaerobically into lactate, acetyl-coenzyme A and carbon dioxide. The extracts could also convert pyruvate into alanine. However, pyruvate is not an intermediate in the metabolism of glyoxylate since no lactate or alanine could be detected in the reaction products and no labelled pyruvate could be obtained when extracts were incubated with [1-(14)C]glyoxylate. 3. The (14)C was incorporated from [1-(14)C]glyoxylate by cell-free extracts into carbon dioxide, glycollate, glycine, glutamate and, in trace amounts, into malate, isocitrate and alpha-oxoglutarate. The (14)C was initially incorporated into isocitrate at the same rate as into glycine. 4. The rate of glyoxylate utilization was increased by the addition of succinate, alpha-oxoglutarate or citrate, and in each case alpha-oxoglutarate became labelled. 5. The results are consistent with the suggestion that the carbon dioxide arises by the oxidation of glyoxylate via reactions catalysed respectively by isocitratase, isocitrate dehydrogenase and alpha-oxoglutarate dehydrogenase.

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Section: Discussionsupporting

confidence: 63%

The metabolism of glyoxylate by cell-free extracts of Pseudomonas sp

Bailey

Hullin

1966

Biochemical Journal

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“…TSA was reduced with NADH to glyceric acid. The pathway of glyoxylate degradation by this allantoingrown pseudomonad appeared to differ from the pathway described by Campbell (1954), which involved formate as product. The observed formation of formate in this instance may have been due to a nonenzymatic decomposition of glyoxylate by hydrogen peroxide produced in the oxidation of TSA, as discussed by Kornberg and Elsden (1961).…”

Section: Tsacontrasting

confidence: 58%

GLYOXYLATE FERMENTATION BYSTREPTOCOCCUS ALLANTOICUS

1964

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Extracts of Streptococcus allantoicus were found to degrade glyoxylate, yielding tartronic semialdehyde and CO2. Tartronic semialdehyde was prepared chemically, and its properties were compared with the enzymatic product: reduction by sodium borohydride yielded glycerate; heating at 100 C yielded glycolaldehyde and CO2; autoxidation yielded mesoxalic semialdehyde; periodate oxidation yielded glyoxylate and a compound presumed to be formate. Tartronic semialdehyde reductase was present in extracts of S. allantoicus and in a species of Pseudomonas grown on allantoin. A scheme for the synthesis of acetate from glyoxylate by S. allantoicus is discussed.

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“…1). We have not examined the metabolism of allantoin by our strain in detail; the exact pathway taken and final products formed by members of the fluorescent group have been shown to depend on the properties of the individual strain (12,19,42; Fig. 1).…”

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confidence: 99%

Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

Woolfolk¹

1975

J Bacteriol

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Pseudomonas putida, strain 40, originally isolated by enrichment on caffeine as the sole source of carbon and nitrogen, has been developed to grow on 0.5% caffeine. The organism will grow on any N-methyl derivative of xanthine containing one or more methyl groups at the 1, 3, or 7 positions. An investigation of the activities of resting cell suspensions and cell-free preparations together with the detection of metabolic intermediates suggest that caffeine is first metabolized by the action of an enzyme which is capable of hydrolytically removing all three methyl groups with the production of methanol and free xanthine. The methanol presumably is oxidized to the final product, CO2, through the sequential action of methanol, formaldehyde, and formate dehydrogenases, which are induced by growth on caffeine. Furthermore, the xanthine would seem to be channeled through conventional pathways of purine degradation through the action of xanthine dehydrogenase and uricase, both induced by growth on caffeine. However, a variety of data suggests that the metabolism of caffeine may be compartmentalized in the cell and metabolized separately from externally added xanthine. Additional studies indicated that the cell is permeable to the methylxanthines. The significance of these findings'is discussed.

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The Mechanism of Allantoin Degradation by a Pseudomonas

Cited by 40 publications

References 20 publications

The metabolism of glyoxylate by cell-free extracts of Pseudomonas sp

The metabolism of glyoxylate by cell-free extracts of Pseudomonas sp

GLYOXYLATE FERMENTATION BYSTREPTOCOCCUS ALLANTOICUS

Metabolism of N-methylpurines by a Pseudomonas putida strain isolated by enrichment on caffeine as the sole source of carbon and nitrogen

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