The measurement of the isoflavone daidzein by time resolved fluorescent immunoassay: a method for assessment of dietary soya exposure

Kohen, Förtüne; Lichter, S.; Gayer, Batya; Deboever, J.; Lu, Ligang

doi:10.1016/s0960-0760(97)00156-8

Cited by 29 publications

(8 citation statements)

References 11 publications

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“…Conjugates of daidzein with keyhole limpet hemocyanin (KLH) 7-(O)-carboxymethyl daidzein (cD-KLH); 6-carboxymethyl genistein-KLH (cG-KLH) and cBA-Ov were prepared as previously described [22,[34][35][36].…”

Section: Preparation Of Estradiol Macromolecular Conjugatesmentioning

confidence: 99%

Membranal effects of phytoestrogens and carboxy derivatives of phytoestrogens on human vascular and bone cells: New insights based on studies with carboxy-biochanin A

Sömjen

Kohen

Lieberherr

et al. 2005

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Preparation Of Estradiol Macromolecular Conjugatesmentioning

confidence: 99%

Membranal effects of phytoestrogens and carboxy derivatives of phytoestrogens on human vascular and bone cells: New insights based on studies with carboxy-biochanin A

Sömjen

Kohen

Lieberherr

et al. 2005

The Journal of Steroid Biochemistry and Molecular Biology

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“…Equol production may enhance the action of isoflavones because it has a lower affinity for serum proteins and a greater affinity for estrogen receptors and exhibits antioxidant activity greater than that of genistein and daidzein (17)(18)(19) As part of the European Union Isoheart project to investigate the potential cardioprotective effects of isolated isoflavones in healthy postmenopausal women, we developed and validated methods for the assessment of the isoflavone metabolites daidzein, genistein, and equol. When the Isoheart project commenced, commercial phytoestrogen assays were not available, but an appropriate monoclonal antibody against daidzein was available (20 ). We therefore prepared monoclonal antibodies to 2 other key isoflavone metabolites, genistein and equol.…”

mentioning

confidence: 99%

Monoclonal Antibody-Based Time-Resolved Fluorescence Immunoassays for Daidzein, Genistein, and Equol in Blood and Urine: Application to the Isoheart Intervention Study

et al. 2007

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“…Samples were analysed for daidzein using a previously published TR-FIA [13]. Based on this established format, similar TR-FIA assays for genistein and equol, using monoclonal antibodies prepared by the Unilever Colworth monoclonal group, were developed.…”

Section: Serum and Urinary Isoflavone Analysismentioning

confidence: 99%

Absorption of isoflavones in humans: effects of food matrix and processing

Pascual‐Teresa¹,

Hallund²,

Talbot

et al. 2006

The Journal of Nutritional Biochemistry

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If soy isoflavones are to be effective in preventing or treating a range of diseases, they must be bioavailable, and thus understanding factors which may alter their bioavailability needs to be elucidated. However, to date there is little information on whether the pharmacokinetic profile following ingestion of a defined dose is influenced by the food matrix in which the isoflavone is given or by the processing method used. Three different foods (cookies, chocolate bars and juice) were prepared, and their isoflavone contents were determined. We compared the urinary and serum concentrations of daidzein, genistein and equol following the consumption of three different foods, each of which contained 50 mg of isoflavones. After the technological processing of the different test foods, differences in aglycone levels were observed. The plasma levels of the isoflavone precursor daidzein were not altered by food matrix. Urinary daidzein recovery was similar for all three foods ingested with total urinary output of 33-34% of ingested dose. Peak genistein concentrations were attained in serum earlier following consumption of a liquid matrix rather than a solid matrix, although there was a lower total urinary recovery of genistein following ingestion of juice than that of the two other foods.

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The measurement of the isoflavone daidzein by time resolved fluorescent immunoassay: a method for assessment of dietary soya exposure

Cited by 29 publications

References 11 publications

Membranal effects of phytoestrogens and carboxy derivatives of phytoestrogens on human vascular and bone cells: New insights based on studies with carboxy-biochanin A

Membranal effects of phytoestrogens and carboxy derivatives of phytoestrogens on human vascular and bone cells: New insights based on studies with carboxy-biochanin A

Monoclonal Antibody-Based Time-Resolved Fluorescence Immunoassays for Daidzein, Genistein, and Equol in Blood and Urine: Application to the Isoheart Intervention Study

Absorption of isoflavones in humans: effects of food matrix and processing

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