The measurement of serum salivary isoamylase as a clinical parameter in Sjögren's syndrome

Kalk, Wwi; Vissink, Arjan; Swaanenburg, Jcjm; Spijkervet, Frederik; Roodenburg, Jan; Bootsma, Hendrika; Kallenberg, Cornelis

doi:10.1093/rheumatology/41.6.706

Cited by 5 publications

(7 citation statements)

References 7 publications

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“…In this study, assessment of salivary glands' function was done by measuring salivary IgA and serum amylase levels. Gland damage can be monitored by elevated gland-specialized enzymes that are released in serum indicating functional loss or altered architecture [34].…”

Section: Discussionmentioning

confidence: 99%

Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathway

AbuBakr¹,

Haggag²,

Sabry³

et al. 2020

Heliyon

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Section: Discussionmentioning

confidence: 99%

Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathway

AbuBakr¹,

Haggag²,

Sabry³

et al. 2020

Heliyon

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“…[45][46][47] The association between salivary proteins and diseases such as periodontal disease, HIVimmunodeficiency and autoimmune diseases has been demonstrated. [48][49][50][51][52] To date, exosome analysis of salivary gland cells and saliva has been restricted to non-neoplastic human salivary gland cell lines 18 and whole saliva. 53 Therefore, the aim of the present study was an initial step to identify proteins secreted as human parotid gland exosome components in vivo in order to gain insight into their biological functions related to health and disease processes.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Human Parotid Gland Exosomes by Multidimensional Protein Identification Technology (MudPIT)

et al. 2009

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Human ductal saliva contributes over a thousand unique proteins to whole saliva. The mechanism by which most of these proteins are secreted by salivary glands remains to be determined. The present study used a mass spectrometry-based, shotgun proteomics approach to explore the possibility that many of the proteins found in saliva are derived from exosomes, membrane-bound vesicles of endosomal origin within multivesicular endosomes. Using MudPIT (multidimensional protein identification technology) mass spectrometry, we catalogued 491 proteins in the exosome fraction of human parotid saliva. Many of these proteins were previously observed in ductal saliva from parotid glands (265 proteins). Furthermore, 72 of the proteins in parotid exosomes overlap with those previously identified as urinary exosome proteins, proteins which are also frequently associated with exosomes from other tissues and cell types. Gene Ontology (GO) and KEGG pathway analyses found that cytosolic proteins comprise the largest category of proteins in parotid exosomes (43%), involved in such processes as phosphatidylinositol signaling system, calcium signaling pathway, inositol metabolism, protein export, and signal transduction among others; whereas the integral plasma membrane proteins and associated/peripheral plasma membrane proteins (26%) were associated with extracellular matrix-receptor interaction, epithelial cell signaling, T-cell and B-cell receptor signaling, cytokine receptor interaction, and antigen processing and presentation among other biological functions. In addition, exosomal proteins were linked to specific diseases (e.g. neurodegenerative disorders, prion disease, cancers, type I and II diabetes). Consequently, parotid glands secrete exosomes that reflect the metabolic and functional status of the gland and may also carry informative protein markers useful in the diagnosis and treatment of systemic diseases. KeywordsExosomes; parotid saliva; acinar cells; MudPIT; protein markers

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“…Studies in SS patients have further demonstrated increased levels of serum salivary isoamylase [15] and prolactin [16,17], the latter of which upregulates cathepsin B and cathepsin D expression in the minor salivary glands [18]. The salivary glands of SS patients have been found to have increased expression of matrix metalloproteinases [19].…”

Section: Introductionmentioning

confidence: 99%

Lysosomal enzyme activities: New potential markers for Sjögren’s syndrome

Sohár

Sohár²,

Hammer

2005

Clinical Biochemistry

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The measurement of serum salivary isoamylase as a clinical parameter in Sjögren's syndrome

Cited by 5 publications

References 7 publications

Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathway

Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathway

Proteomic Analysis of Human Parotid Gland Exosomes by Multidimensional Protein Identification Technology (MudPIT)

Lysosomal enzyme activities: New potential markers for Sjögren’s syndrome

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