The measurement of radioactive sulphur (35S) in biological material

The uptake of 35S into rat tissues after injection of [35S]methionine

“…obtained on the same sample of tissue by the Canius bomb procedure. Values obtained by this method for the sulphur content of Wistar albino rat (150-165 g.) tissues showed good agreement with values previously reported by Young et al (1949) ( Table 1).…”

“…This agrees with Folch & Lees (1951), who found that methionine resisted oxidation with nitric acid, but was completely oxidized by aqua regia. Young et al (1949) also found that the Pirie method gave low values for certain sulphur compounds.…”

“…Oxidations by the Carius method were carried out under the conditions described by Young, Edson & McCarter (1949).…”

Analytical Nuclear Chemistry

“…Sul fur.-Young et al (193) have measured S35 in biological materials by oxidizing with the Carius method followed by precipitation of benzidine sul fate which is then measured in an ionization chamber. Mean recovery of 535 was 99 per cent and the standard deviation of a single measurement was 2.8 per cent.…”

Biochemical studies of toxic agents. 5. Observations on the fate of 35S-labelled arylsulphuric acids following their administration to the rat

“…Radioactive sulphate was separated in the form of benzidine sulphate preparatory to the measurement of its 85S content. Although detailed studies of this method of measuring 35S have already been reported (Henriques, Kistiakowsky, Margnetti & Schneider, 1946;Young, Edson & McCarter, 1949), it was necessary to modify these procedures in order to meet the requirements of the present investigation.…”