The Measurement of Proliferation in Tissue Cultures by Enumeration of Cell Nuclei

Sanford, Katherine K.; Earle, Wilton R.; Evans, Virginia J.; Waltz, H.; Shannon, John E.

doi:10.1093/jnci/11.4.773

Cited by 141 publications

(2 citation statements)

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“…Previous theoretical studies (Croughan et al, 1987) assert that cell death is the main driver of biomass reduction at higher rotor speeds but without providing experimental validation. Our understanding from experimentalists and the literature is that dead cells are difficult to count accurately because they may disintegrate into the surrounding media during the experimental period, evading measurement post-experiment (Sanford et al, 1951). Assumption I introduces the alternative mechanism that mechanical stresses suppress cells' proliferation rates while disregarding any changes in cell shape.…”

Section: Assumption the Only Impact Of Mechanical Stress On Cellmentioning

confidence: 99%

Modeling the biomechanics of cells on microcarriers in a stirred-tank bioreactor: an ABM-CFD coupling approach

Cantarero-Rivera,

Camphuijsen,

Potter

et al. 2024

Front. Food. Sci. Technol.

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Highly productive and efficient biomass growth in bioreactors is an essential bioprocess outcome in many industrial applications. Large-scale biomass creation in the cultivated meat industry will be critical given the demand size in the conventional meat and seafood sectors. However, many challenges must be overcome before cultivated meat and seafood become commercially viable, including cost reductions of cell culture media, bioprocess design innovation and optimization, and scaling up in the longer term. Computational modeling and simulation can help to address many of these challenges and can be a far cheaper and faster alternative to performing physical experiments. Computer modeling can also help researchers pinpoint system interactions that matter and guide researchers to identify those parameters that should be changed in later designs for eventual optimization. This work developed a computational model that combines agent-based modeling and computational fluid dynamics to study biomass growth as a function of the operative conditions of stirred-tank bioreactors. The focus was to analyze how the mechanical stress induced by rotor speed can influence the growth of cells attached to spherical microcarriers. The computer simulation results reproduced observations from physical experiments that high rotor speeds reduce cell growth rates and induce cell death under the high mechanical stresses induced at these stir speeds. Moreover, the results suggest that modeling cell death and cell quiescence is required to recapitulate these observations from physical experiments. These simulation outcomes are the first step towards more comprehensive models that, combined with experimental observations, will improve our knowledge of biomass production in bioreactors for cultivated meat and other industries.

show abstract

Section: Assumption the Only Impact Of Mechanical Stress On Cellmentioning

confidence: 99%

Modeling the biomechanics of cells on microcarriers in a stirred-tank bioreactor: an ABM-CFD coupling approach

Cantarero-Rivera,

Camphuijsen,

Potter

et al. 2024

Front. Food. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Our understanding from experimentalists and the literature is that dead cells are difficult to count accurately because they may disintegrate into the surrounding media during the experimental period evading measurement post-experiment [39]. Assumption I introduces the alternative mechanism that mechanical stresses suppresses cells' proliferation rates.…”

Section: IXmentioning

confidence: 99%

Modeling the biomechanics of cells on microcarriers in a stirred-tank bioreactor

Camphuijsen

Rivera

Potter³

et al. 2022

Preprint

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Highly productive and efficient growth of biomass in bioreactors is an essential bioprocess outcome in many industrial applications. In the nascent cultivated meat industry, large-scale biomass creation will be critical given the size of demand in the conventional meat and seafood sectors. However, there are many challenges that must be overcome before cultivated meat and seafood become commercially viable including cost reductions of cell culture media, bioprocess design innovation and optimization, and scaling up in the longer term. Computational modelling and simulation can help to address many of these challenges, and can be a far cheaper and faster alternative to performing physical experiments. Computer modelling can also help researchers pinpoint system interactions that matter, and guide researchers to identify those parameters that should be changed in later designs for eventual optimization. In this work, a computational model that combines agent-based modeling and computational fluid dynamics was developed to study biomass growth as a function of the operative conditions of stirred-tank bioreactors. The focus was to analyze how the mechanical stress induced by rotor speed can influence the growth of cells attached to spherical microcarriers. The computer simulation results reproduced observations from physical experiments that high rotor speeds reduce cell growth rates and induce cell death under the high mechanical stresses induced at these stir speeds. Moreover, the results suggest that modeling both cell death and cell quiescence are required to recapitulate these observations from physical experiments. These simulation outcomes are the first step towards more comprehensive models that, in combination with experimental observations, will improve our knowledge of biomass production in bioreactors for cultivated meat and other industries.

show abstract

Long‐Term, Large‐Scale, Tissue Culture

Earle¹

1954

Ciba Foundation Symposium ‐ Preservation and Transplantation of Normal Tissues

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The Measurement of Proliferation in Tissue Cultures by Enumeration of Cell Nuclei

Cited by 141 publications

References 0 publications

Modeling the biomechanics of cells on microcarriers in a stirred-tank bioreactor: an ABM-CFD coupling approach

Modeling the biomechanics of cells on microcarriers in a stirred-tank bioreactor: an ABM-CFD coupling approach

Modeling the biomechanics of cells on microcarriers in a stirred-tank bioreactor

Long‐Term, Large‐Scale, Tissue Culture

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